##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2866447
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09834160547884	31.0	31.0	33.0	30.0	34.0
2	31.2477118886203	31.0	31.0	34.0	28.0	34.0
3	31.30878087053415	31.0	31.0	34.0	28.0	34.0
4	35.26541533822185	37.0	35.0	37.0	33.0	37.0
5	35.121205450510686	37.0	35.0	37.0	32.0	37.0
6	35.16817788711949	37.0	35.0	37.0	32.0	37.0
7	35.01400828272771	37.0	35.0	37.0	32.0	37.0
8	35.071343373870164	37.0	35.0	37.0	32.0	37.0
9	36.578551775072064	39.0	35.0	39.0	32.0	39.0
10	36.42963257300763	38.0	35.0	39.0	32.0	39.0
11	36.45391943405896	38.0	35.0	39.0	32.0	39.0
12	36.296427947211306	38.0	35.0	39.0	32.0	39.0
13	36.36429419417139	38.0	35.0	39.0	32.0	39.0
14	37.30825548143747	39.0	36.0	41.0	32.0	41.0
15	37.363819041482365	39.0	36.0	41.0	32.0	41.0
16	37.33483472745179	39.0	36.0	41.0	32.0	41.0
17	37.407078868020236	39.0	36.0	41.0	32.0	41.0
18	37.411688756150035	39.0	36.0	41.0	32.0	41.0
19	37.499552581994365	39.0	36.0	41.0	32.0	41.0
20	37.45445773112149	39.0	36.0	41.0	32.0	41.0
21	37.33640949928605	39.0	36.0	41.0	31.0	41.0
22	37.2915822270567	39.0	36.0	41.0	31.0	41.0
23	37.198868494690466	39.0	36.0	40.0	31.0	41.0
24	37.11018658290211	39.0	36.0	40.0	31.0	41.0
25	37.02391497208914	39.0	36.0	40.0	31.0	41.0
26	36.77526359287299	39.0	35.0	40.0	30.0	41.0
27	36.62239036688974	38.0	35.0	40.0	30.0	41.0
28	36.481679584516996	38.0	35.0	40.0	30.0	41.0
29	36.35194894585527	38.0	35.0	40.0	30.0	41.0
30	36.17747755322181	38.0	35.0	40.0	30.0	41.0
31	35.954038571095154	38.0	35.0	40.0	29.0	41.0
32	35.68441872464413	38.0	34.0	40.0	28.0	41.0
33	35.84678628280935	38.0	34.0	40.0	29.0	41.0
34	35.84143819857824	38.0	35.0	40.0	29.0	41.0
35	35.7441826065509	38.0	34.0	40.0	28.0	41.0
36	35.67159483499957	38.0	34.0	40.0	28.0	41.0
37	35.53804588049247	38.0	34.0	40.0	27.0	41.0
38	35.42554423647115	38.0	34.0	40.0	27.0	41.0
39	35.37950221999569	38.0	34.0	40.0	26.0	41.0
40	35.23936078357632	38.0	34.0	40.0	25.0	41.0
41	35.12671680306666	38.0	34.0	40.0	25.0	41.0
42	34.9464532224039	38.0	34.0	40.0	24.0	41.0
43	34.63546508970862	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	18.0
16	30.0
17	87.0
18	192.0
19	426.0
20	884.0
21	1828.0
22	3537.0
23	6033.0
24	10083.0
25	15967.0
26	24713.0
27	36033.0
28	50745.0
29	68827.0
30	89041.0
31	109546.0
32	133866.0
33	163232.0
34	199341.0
35	241645.0
36	301597.0
37	391173.0
38	501724.0
39	515875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79876585891872	22.604987986870157	13.632032966247065	22.964213187964056
2	17.425125948604666	22.93431554813328	36.69420714912921	22.946351354132837
3	19.576988515747892	26.348472516673084	32.0733995779444	22.001139389634623
4	12.862125132611906	15.773743592677626	36.191110458347914	35.17302081636255
5	11.119479969453474	40.608983874462005	35.21258198738717	13.058954168697346
6	31.390672843419047	36.67735702072984	15.701458983891905	16.230511151959202
7	24.735709399127213	35.00015873309362	21.87956030584204	18.38457156193713
8	27.34768861939537	33.90524925107633	20.419285617351377	18.32777651217692
9	25.327557076757394	13.691549154754998	23.556130638382637	37.42476313010497
10	16.047741332737008	27.650467634671077	34.19396904948879	22.107821983103122
11	31.613143379242665	21.98125414493971	25.238317680389695	21.16728479542793
12	19.99259012987158	27.392971159069052	32.01936055332612	20.595078157733248
13	30.511675255115478	21.127514306038105	28.61511132073958	19.74569911810684
14	20.72112269998364	22.38084290412486	28.64581134763699	28.25222304825451
15	22.804677707280128	29.092008329475476	26.94506474391468	21.158249219329715
16	21.76133031589281	28.392954762463773	27.197572465145875	22.64814245649754
17	19.422616221405804	27.332722356282886	28.313518442866727	24.931142979444587
18	20.464568157025056	26.826171912475623	32.100122555902836	20.609137374596497
19	19.99238081150637	25.175138420490594	33.28946950702385	21.543011260979185
20	23.84596680140955	24.305176408285238	32.18894331554011	19.6599134747651
21	23.282586421447878	25.178661946304953	30.890087973020258	20.64866365922691
22	21.75260871734241	24.82107640573853	31.861953142688492	21.564361734230562
23	20.97397928515685	26.506612541588943	32.75347494651044	19.76593322674377
24	19.84090408788301	25.913474067373304	31.232567704897384	23.013054139846297
25	20.709959053839125	26.705290556567068	32.35653755328461	20.2282128363092
26	22.05786466660643	25.812931479284284	31.030331277710697	21.098872576398588
27	20.786813780265256	27.21728327786978	30.605205677969977	21.39069726389499
28	20.68260812078507	26.395987785575663	30.9737804327099	21.947623660929366
29	19.974239886521534	26.47716842488279	31.71099971497816	21.837591973617513
30	19.3071073702043	26.601259329057893	33.53304631133944	20.55858698939837
31	20.56716904237197	26.103186279041612	32.50319995450815	20.826444724078275
32	21.340077105908463	26.261535622322686	30.743913981315547	21.654473290453303
33	22.1834556857322	26.178924640853296	30.386154008778117	21.251465664636395
34	20.792290944154907	28.529534995763044	30.26590060796519	20.412273452116857
35	19.554765882641473	29.428766692703544	30.41500505678284	20.60146236787214
36	20.726739409450097	26.02594780227927	32.282369079211996	20.964943709058637
37	20.554644826853593	26.226021273025456	31.631249417833295	21.588084482287652
38	20.75796273226053	25.824269557399802	32.28359010300906	21.13417760733061
39	21.413338533731828	25.463265150201625	32.16225522397588	20.961141092090664
40	19.811704175936274	26.24088287695534	31.577035961244004	22.370376985864382
41	20.702109615143765	25.764613823315063	31.3612636131071	22.172012948434073
42	18.660557826466004	27.383098309509997	31.50370476063224	22.45263910339176
43	19.183330443577013	27.015709692172923	31.3329707474096	22.467989116840464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	276.0
2	369.0
3	1178.5
4	1988.0
5	1988.0
6	3508.0
7	5028.0
8	5317.0
9	5606.0
10	8684.0
11	11762.0
12	11762.0
13	20900.5
14	30039.0
15	48172.5
16	66306.0
17	65499.0
18	64692.0
19	64692.0
20	69051.5
21	73411.0
22	61089.0
23	48767.0
24	53891.5
25	59016.0
26	59016.0
27	64508.5
28	70001.0
29	75219.5
30	80438.0
31	87591.0
32	94744.0
33	94744.0
34	104225.5
35	113707.0
36	126102.5
37	138498.0
38	143657.0
39	148816.0
40	148816.0
41	155084.5
42	161353.0
43	180432.0
44	199511.0
45	239924.0
46	280337.0
47	280337.0
48	283264.0
49	286191.0
50	259233.5
51	232276.0
52	214916.0
53	197556.0
54	197556.0
55	172557.0
56	147558.0
57	122136.5
58	96715.0
59	85118.5
60	73522.0
61	73522.0
62	64864.5
63	56207.0
64	48614.5
65	41022.0
66	35096.5
67	29171.0
68	29171.0
69	24550.5
70	19930.0
71	16576.0
72	13222.0
73	10722.5
74	8223.0
75	8223.0
76	6359.5
77	4496.0
78	3561.5
79	2627.0
80	2053.0
81	1479.0
82	1479.0
83	1124.0
84	769.0
85	613.5
86	458.0
87	362.5
88	267.0
89	267.0
90	205.5
91	144.0
92	85.0
93	26.0
94	19.0
95	12.0
96	12.0
97	8.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2866447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.07409385200614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9532143322833	31.11514480474013
2	13.919109772603989	11.712678618178323
3	5.008741833690327	6.322148219734684
4	2.3244580509884196	3.911978647693522
5	1.2395830739313813	2.60771672949736
6	0.7605467997779883	1.9199590459621205
7	0.4806837605500468	1.4157033558162542
8	0.3430634874442975	1.1547268294342323
9	0.24426409294294807	0.9249471334041073
>10	1.432455056118882	11.756728993216758
>50	0.16942342342438174	4.998364183540046
>100	0.11082640474209594	8.76711104325107
>500	0.006438669850633639	1.8323827503378267
>1k	0.005853336227846913	4.6508810491110495
>5k	9.198099786616577E-4	2.7316340050389893
>10k+	4.1809544484620805E-4	4.17789459104343
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31520	1.099619145234501	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31005	1.081652652220676	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27822	0.9706092594769763	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17074	0.5956502945981559	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11330	0.39526284630415287	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9264	0.32318755588364273	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9126	0.3183732334838216	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8701	0.30354651594814064	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8618	0.30065094522940766	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6993	0.24396055465180413	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6580	0.22955247384654243	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6476	0.22592428884957583	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6378	0.2225054222178188	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5406	0.18859584705386145	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5088	0.17750197369775195	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5013	0.1748854941326318	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4496	0.15684922833040346	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3843	0.13406841291675722	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3817	0.13316136666751557	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3555	0.1240211313866958	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3461	0.12074181033174519	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3197	0.11153180226252221	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3152	0.10996191452345011	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3014	0.10514759212362901	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2999	0.10462429621060497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.488639420160219E-5	0.0	0.0	0.0	0.0
3	3.488639420160219E-5	0.0	0.0	0.0	0.0
4	3.488639420160219E-5	0.0	0.0	0.0	0.0
5	3.488639420160219E-5	3.488639420160219E-5	0.0	1.0465918260480658E-4	0.0
6	3.488639420160219E-5	3.488639420160219E-5	0.0	1.0465918260480658E-4	0.0
7	3.488639420160219E-5	3.488639420160219E-5	0.0	1.0465918260480658E-4	0.0
8	3.488639420160219E-5	3.488639420160219E-5	0.0	1.0465918260480658E-4	0.0
9	3.488639420160219E-5	3.488639420160219E-5	0.0	1.3954557680640877E-4	0.0
10	3.488639420160219E-5	3.488639420160219E-5	0.0	2.4420475941121537E-4	0.0
11	3.488639420160219E-5	3.488639420160219E-5	0.0	4.884095188224307E-4	0.0
12	3.488639420160219E-5	3.488639420160219E-5	0.0	6.977278840320438E-4	0.0
13	3.488639420160219E-5	3.488639420160219E-5	0.0	9.07046249241657E-4	0.0
14	3.488639420160219E-5	3.488639420160219E-5	0.0	0.0012907965854592812	0.0
15	3.488639420160219E-5	3.488639420160219E-5	0.0	0.0017792061042817117	0.0
16	3.488639420160219E-5	6.977278840320438E-5	0.0	0.0030002299013377884	0.0
17	3.488639420160219E-5	6.977278840320438E-5	0.0	0.0041165945157890585	0.0
18	3.488639420160219E-5	6.977278840320438E-5	0.0	0.004570117640409887	0.0
19	3.488639420160219E-5	6.977278840320438E-5	0.0	0.005302731918643533	0.0
20	3.488639420160219E-5	6.977278840320438E-5	0.0	0.006628414898304417	0.0
21	3.488639420160219E-5	1.0465918260480658E-4	0.0	0.009907735953255023	0.0
22	3.488639420160219E-5	1.0465918260480658E-4	0.0	0.017547856283405903	0.0
23	3.488639420160219E-5	1.7443197100801096E-4	0.0	0.028048660938088162	0.0
24	3.488639420160219E-5	1.7443197100801096E-4	0.0	0.04343356078099473	0.0
25	3.488639420160219E-5	1.7443197100801096E-4	0.0	0.05058527159232318	0.0
26	3.488639420160219E-5	1.7443197100801096E-4	0.0	0.05948130211373174	0.0
27	3.488639420160219E-5	2.0931836520961315E-4	0.0	0.13012625037197617	0.0
28	3.488639420160219E-5	2.0931836520961315E-4	0.0	0.20610881694306576	0.0
29	3.488639420160219E-5	2.0931836520961315E-4	0.0	0.28871979841245976	0.0
30	3.488639420160219E-5	2.0931836520961315E-4	0.0	0.4488483477978138	0.0
31	3.488639420160219E-5	2.0931836520961315E-4	0.0	0.6898784453366834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7120	0.0	29.72472	1
GTATTGG	1155	0.0	20.021645	1
ACGTTTA	150	1.2732926E-11	18.5	26
GGACCCT	1735	0.0	17.59366	6
CGCACTA	75	2.0686245E-4	17.266666	19
CGAACTA	215	0.0	17.209303	24
ATTGGAC	1305	0.0	17.153257	3
CGTCTGT	790	0.0	17.094936	34
TGGACCC	1835	0.0	16.93733	5
GTATCAA	12650	0.0	16.788935	2
TTGGACC	1930	0.0	16.199482	4
GACCCTC	1875	0.0	15.589333	7
GTACCGT	110	1.453295E-5	15.136364	6
CACGGTA	75	0.0041063954	14.8	32
ACGGACC	155	1.2120472E-7	14.32258	8
TATTGGA	1565	0.0	14.303513	2
TATATCG	80	0.0063016503	13.875	5
TGCGTCT	865	0.0	13.6878605	32
TCACGTT	190	7.1395334E-9	13.631579	24
AAGACCG	140	9.569923E-6	13.214286	5
>>END_MODULE