FastQCFastQC Report
Wed 25 May 2016
SRR2088199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984567
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171911.7460467393280499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160791.6331036892359787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137161.3930997077903282No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77480.7869449209652568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.5262211713372478No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48010.4876255247230508No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT46340.4706637537110222No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA45000.45705370990496325No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT44890.4559364675029734No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37050.3763075544884198No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT33020.33537585557915306No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG32620.3313131559355534No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32330.3283676986939436No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27640.28073254537273745No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA23090.23451933692679117No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22860.23218328463172136No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22510.22862842244357165No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20490.20811178924339327No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.20628357440377346No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17920.18200894403326537No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16880.17144592495990624No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16110.16362522814597685No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15760.16007036595782714No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15570.1581405836271173No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15380.15621080129640744No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.1408741101418187No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12690.12888914619319963No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12580.12777190379120973No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA12520.1271624988446698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.12238882676344016No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA11790.11974807199510039No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11040.11213051016335099No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC11020.11192737518117102No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC10870.11040386281482113No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC10810.10979445786828118No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC10760.10928662041283121No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC10490.10654429815340144No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC10380.10542705575141154No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG10360.10522392076923155No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA9990.10146592359890184No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC9900.10055181617909191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA355.630209E-737.026
GGTATCA38250.030.5673221
GCCCCGA1050.026.4285711
CCGAATT951.0913936E-1125.3157914
ATTGGAC4300.024.0930233
CGAATTA1002.0008883E-1124.0515
GTATACG701.9223444E-723.7857151
GTATTGG4150.023.6265071
TGCGTCG400.001930991323.12500210
CATTGCG502.701721E-422.19999929
AGCCGTC603.7255886E-521.58333212
TATTGGA4850.020.9793822
CTAGATA450.003825424220.5555554
TTGGACC7150.019.9230774
TATACGG656.900845E-519.9230772
TATACCG759.26356E-619.7333345
TCACGTT759.26356E-619.73333424
CCCGAAT1355.638867E-1119.18518413
TACAGAG2050.018.9512215
TGGACCC7900.018.55
Produced by FastQC (version 0.11.5)