##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	984567
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.819879195626097	31.0	31.0	33.0	28.0	34.0
2	30.952052018806235	31.0	31.0	34.0	28.0	34.0
3	31.00179672891738	31.0	31.0	34.0	27.0	34.0
4	34.98031723590167	35.0	35.0	37.0	32.0	37.0
5	34.85692187530153	35.0	35.0	37.0	32.0	37.0
6	34.93113114699152	35.0	35.0	37.0	32.0	37.0
7	34.74893024040009	35.0	35.0	37.0	31.0	37.0
8	34.83691409523171	35.0	35.0	37.0	32.0	37.0
9	36.24820250932643	38.0	35.0	39.0	32.0	39.0
10	36.102855366877016	38.0	35.0	39.0	31.0	39.0
11	36.14491344926247	38.0	35.0	39.0	31.0	39.0
12	35.96456716505835	38.0	35.0	39.0	30.0	39.0
13	36.01676168305458	38.0	35.0	39.0	30.0	39.0
14	36.82948544893339	38.0	36.0	40.0	31.0	41.0
15	36.89156349948759	38.0	36.0	40.0	31.0	41.0
16	36.83644993179743	38.0	36.0	40.0	31.0	41.0
17	36.99076040533554	38.0	36.0	40.0	31.0	41.0
18	37.016946535888366	38.0	36.0	40.0	31.0	41.0
19	37.134994368082616	39.0	36.0	40.0	31.0	41.0
20	37.0997138843776	39.0	36.0	40.0	31.0	41.0
21	36.97756475689313	39.0	36.0	40.0	31.0	41.0
22	36.9339344097456	39.0	36.0	40.0	31.0	41.0
23	36.79586864073242	39.0	35.0	40.0	31.0	41.0
24	36.69869089660734	38.0	35.0	40.0	30.0	41.0
25	36.61207820290544	38.0	35.0	40.0	30.0	41.0
26	36.315390420357375	38.0	35.0	40.0	30.0	41.0
27	36.12583602741103	38.0	35.0	40.0	30.0	41.0
28	35.95209366147758	38.0	34.0	40.0	29.0	41.0
29	35.78919565656781	38.0	34.0	40.0	29.0	41.0
30	35.57940698804652	38.0	34.0	40.0	28.0	41.0
31	35.26388452995073	37.0	34.0	40.0	27.0	41.0
32	34.98167417758263	37.0	33.0	40.0	26.0	41.0
33	35.09735142453485	37.0	33.0	40.0	27.0	41.0
34	35.03734027242432	37.0	33.0	40.0	26.0	41.0
35	34.90968720259769	37.0	33.0	40.0	25.0	41.0
36	34.801567592657484	37.0	33.0	40.0	25.0	41.0
37	34.6433934917583	37.0	33.0	40.0	24.0	41.0
38	34.485202124385644	37.0	33.0	40.0	24.0	41.0
39	34.36879054447285	37.0	33.0	40.0	23.0	41.0
40	34.161740135511344	37.0	33.0	40.0	22.0	41.0
41	33.978833334856844	37.0	33.0	40.0	21.0	41.0
42	33.703555979430554	37.0	33.0	40.0	19.0	41.0
43	33.36629909391641	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	13.0
17	47.0
18	101.0
19	250.0
20	507.0
21	1045.0
22	1887.0
23	3091.0
24	4998.0
25	7719.0
26	11409.0
27	16195.0
28	22062.0
29	29392.0
30	36432.0
31	43777.0
32	52379.0
33	63064.0
34	74254.0
35	87791.0
36	109177.0
37	138771.0
38	161322.0
39	118875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.292026850381944	24.000601279547254	14.810977820706972	20.89639404936383
2	18.074036606955136	24.062862151585417	36.1224782061556	21.740623035303845
3	20.822859185814675	26.459448671344866	32.820417503328876	19.897274639511583
4	13.278832217614445	16.242774742602588	34.641827321045696	35.836565718737276
5	10.434129927165953	41.74840310512134	34.359368128324434	13.45809883938828
6	31.38486258426293	35.987393443005914	14.924428708254492	17.703315264476668
7	23.28069090270139	36.8065352586467	21.0245722231194	18.888201615532513
8	27.924661297809088	33.93207369330884	20.207969594755866	17.93529541412621
9	24.756263413256793	13.572463834355611	24.826852819564333	36.844419932823264
10	16.628426506271285	28.23393430817811	33.23755518923547	21.900083996315132
11	30.566025471095415	21.67856529824786	25.70856020971656	22.046849020940172
12	19.93292482888417	28.13622638174954	32.1046713936177	19.826177395748587
13	31.656961892893015	20.936005370888928	29.99775535844691	17.409277377771144
14	20.501601211497032	22.877975800529573	29.40378867055264	27.216634317420755
15	22.754165028890874	28.975580128117233	28.35124476038705	19.919010082604842
16	20.733073523691125	28.89656062004922	28.23616879298209	22.134197063277565
17	18.300430544594732	27.24791710467647	28.92652302992077	25.52512932080803
18	19.52299843484496	26.13209664756182	34.324632046371654	20.02027287122156
19	18.491986832790456	24.357712578219665	36.20393533400977	20.94636525498011
20	24.080027057579624	23.026670607485322	34.75995031318336	18.133352021751694
21	23.456097959813807	24.012586243495875	33.00557503958593	19.525740757104394
22	21.274834521165143	23.593214072785294	34.10788702038561	21.02406438566395
23	19.988177544037125	26.367530091908424	35.43730391126251	18.20698845279194
24	18.483759866012168	25.03496460880773	33.51341249503589	22.967863030144215
25	19.67819356123047	26.35808431523705	34.82363313009678	19.140088993435693
26	21.717871917299686	24.88373061457473	33.15041028188026	20.247987186245325
27	19.982896034500445	26.77999567322488	32.68594214512572	20.55116614714895
28	19.653411093404515	26.171606401595827	32.83707457186763	21.337907933132026
29	18.582077197387278	25.8887409389102	33.99575651022226	21.53342535348026
30	17.69833845741326	26.142354964161914	36.429516731720646	19.729789846704186
31	19.505325691395303	25.428233934308174	34.94277179714535	20.123668577151175
32	20.870697474118064	25.9039760625737	32.39271679834892	20.832609664959318
33	22.061677874639308	25.816424885254126	31.66041518759008	20.461482052516487
34	19.81165324452272	29.415773634501257	31.84059591678372	18.9319772041923
35	18.08957643309191	30.327951271980474	31.963086311038253	19.619385983889366
36	19.236374974989005	25.772243026629983	34.805147846718405	20.18623415166261
37	19.343731813071127	26.38225737811647	33.48101246537818	20.79299834343422
38	19.991529271243095	25.56433437236877	34.18558615106945	20.258550205318684
39	21.114053182769684	25.15847067797316	33.940503795069304	19.786972344187852
40	19.017801734163342	26.242703645358823	32.65425308790565	22.085241532572187
41	20.140427213181024	25.757617307913023	32.461376422325756	21.640579056580204
42	17.254386953858905	27.723456097959815	32.95072859439733	22.071428353783947
43	18.131828509385343	27.670641002593015	32.228075895292044	21.969454592729594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	102.0
2	123.0
3	378.0
4	633.0
5	633.0
6	1238.0
7	1843.0
8	2083.0
9	2323.0
10	3650.0
11	4977.0
12	4977.0
13	9345.5
14	13714.0
15	23418.5
16	33123.0
17	32253.5
18	31384.0
19	31384.0
20	33507.5
21	35631.0
22	27880.0
23	20129.0
24	21334.0
25	22539.0
26	22539.0
27	23980.0
28	25421.0
29	26041.5
30	26662.0
31	27891.0
32	29120.0
33	29120.0
34	31144.5
35	33169.0
36	35955.0
37	38741.0
38	40669.0
39	42597.0
40	42597.0
41	44698.5
42	46800.0
43	54851.0
44	62902.0
45	83983.0
46	105064.0
47	105064.0
48	107171.0
49	109278.0
50	97188.5
51	85099.0
52	78199.5
53	71300.0
54	71300.0
55	59734.5
56	48169.0
57	38170.5
58	28172.0
59	24023.0
60	19874.0
61	19874.0
62	17164.5
63	14455.0
64	12558.5
65	10662.0
66	9020.0
67	7378.0
68	7378.0
69	6179.5
70	4981.0
71	4232.0
72	3483.0
73	2830.5
74	2178.0
75	2178.0
76	1661.0
77	1144.0
78	914.5
79	685.0
80	523.5
81	362.0
82	362.0
83	273.0
84	184.0
85	136.0
86	88.0
87	69.5
88	51.0
89	51.0
90	45.5
91	40.0
92	22.0
93	4.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	984567.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.91991481728986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00245423606391	38.81630706983603
2	10.99090612722162	10.533665707625786
3	3.269684544274122	4.700490145230754
4	1.370727333372723	2.627405482118071
5	0.7701046971129325	1.8451675743023266
6	0.4736835088429872	1.361932403646655
7	0.30640044709924497	1.0277878327482743
8	0.24200894752711868	0.9277638520417198
9	0.1796472780978316	0.7747814037245465
>10	1.209849722410583	11.082975876788348
>50	0.1004810010665633	3.2667503366355968
>100	0.06613240773238326	6.173020919076527
>500	0.009599865631028937	3.4275932484406275
>1k	0.0072532318101107525	7.309384260095836
>5k	4.2666069471239713E-4	1.3217022013507373
>10k+	6.399910420685958E-4	4.803271686338135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17191	1.7460467393280499	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16079	1.6331036892359787	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13716	1.3930997077903282	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7748	0.7869449209652568	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5181	0.5262211713372478	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4801	0.4876255247230508	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4634	0.4706637537110222	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4500	0.45705370990496325	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4489	0.4559364675029734	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3705	0.3763075544884198	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3302	0.33537585557915306	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3262	0.3313131559355534	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3233	0.3283676986939436	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2764	0.28073254537273745	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2309	0.23451933692679117	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2286	0.23218328463172136	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2251	0.22862842244357165	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2049	0.20811178924339327	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2031	0.20628357440377346	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1792	0.18200894403326537	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1688	0.17144592495990624	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1611	0.16362522814597685	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1576	0.16007036595782714	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1557	0.1581405836271173	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1538	0.15621080129640744	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.1408741101418187	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1269	0.12888914619319963	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1258	0.12777190379120973	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1252	0.1271624988446698	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1205	0.12238882676344016	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1179	0.11974807199510039	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1104	0.11213051016335099	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1102	0.11192737518117102	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1087	0.11040386281482113	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1081	0.10979445786828118	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1076	0.10928662041283121	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1049	0.10654429815340144	No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC	1038	0.10542705575141154	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1036	0.10522392076923155	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	999	0.10146592359890184	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	990	0.10055181617909191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.0156749108999184E-4	0.0
11	0.0	0.0	0.0	3.047024732699755E-4	0.0
12	0.0	0.0	0.0	0.0010156749108999185	0.0
13	0.0	0.0	0.0	0.0017266473485298614	0.0
14	0.0	0.0	0.0	0.002031349821799837	0.0
15	0.0	0.0	0.0	0.002539187277249796	0.0
16	0.0	0.0	0.0	0.004875239572319608	0.0
17	0.0	0.0	0.0	0.007820696813929372	0.0
18	0.0	0.0	0.0	0.009344209180279249	0.0
19	0.0	0.0	0.0	0.010156749108999184	0.0
20	0.0	0.0	0.0	0.013305341332788932	0.0
21	0.0	0.0	0.0	0.018485283378378514	0.0
22	0.0	0.0	0.0	0.03443137947950724	0.0
23	0.0	0.0	0.0	0.0560652550816755	0.0
24	0.0	0.0	0.0	0.08836371724829291	0.0
25	0.0	0.0	0.0	0.09852046635729209	0.0
26	0.0	0.0	0.0	0.11050543030591113	0.0
27	0.0	0.0	0.0	0.19115001823136465	0.0
28	0.0	0.0	0.0	0.26864601393302845	0.0
29	0.0	0.0	0.0	0.36523669795961067	0.0
30	0.0	0.0	0.0	0.5662387628267045	0.0
31	0.0	0.0	0.0	0.8385412064389727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	35	5.630209E-7	37.0	26
GGTATCA	3825	0.0	30.567322	1
GCCCCGA	105	0.0	26.42857	11
CCGAATT	95	1.0913936E-11	25.31579	14
ATTGGAC	430	0.0	24.093023	3
CGAATTA	100	2.0008883E-11	24.05	15
GTATACG	70	1.9223444E-7	23.785715	1
GTATTGG	415	0.0	23.626507	1
TGCGTCG	40	0.0019309913	23.125002	10
CATTGCG	50	2.701721E-4	22.199999	29
AGCCGTC	60	3.7255886E-5	21.583332	12
TATTGGA	485	0.0	20.979382	2
CTAGATA	45	0.0038254242	20.555555	4
TTGGACC	715	0.0	19.923077	4
TATACGG	65	6.900845E-5	19.923077	2
TATACCG	75	9.26356E-6	19.733334	5
TCACGTT	75	9.26356E-6	19.733334	24
CCCGAAT	135	5.638867E-11	19.185184	13
TACAGAG	205	0.0	18.951221	5
TGGACCC	790	0.0	18.5	5
>>END_MODULE