FastQCFastQC Report
Wed 25 May 2016
SRR2088198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306008
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT351761.0640022649672958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340261.0292171101824317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306060.9257690846483131No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG178930.5412267604918076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124290.375951903322678No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA95730.28956372761348426No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94050.2844820702188259No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT92900.2810035547403394No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT90520.2738045400979066No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA73730.22301821411200456No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT72170.21829953224553603No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67960.20556514079820737No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT67780.20502067750592254No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57620.1742887494525119No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56650.1713546972663103No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT49650.15018112478856677No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA49620.1500903809065193No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.12601300420325662No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38380.11609167309939963No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC37600.11373233216616536No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35560.10756174818693724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79500.029.088051
GTATTGG10600.022.8632091
ATTGGAC11400.020.1228083
TTGGACC17200.018.54
ACGTTTA1800.018.526
GGACCCT17750.018.0309856
TGGACCC18650.016.6648795
GTATCAA139200.016.6526572
TATACCG2900.016.5862065
GACCCTC18900.015.7592597
CGCCGTT957.066084E-515.57894825
ATCACGT2150.015.48837223
CGTCTGT8350.015.28742534
TATTGGA14900.015.27181152
TCACGTT2450.014.34693824
CGTCGAG1304.44975E-614.23076910
TGACGTA1051.6571152E-414.09523820
ACCGTTA1051.6571152E-414.0952388
CGCCGGA2500.014.05999914
GCGTTAT1854.9076334E-914.01
Produced by FastQC (version 0.11.5)