##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3306008
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13112732939545	31.0	31.0	33.0	30.0	34.0
2	31.285127864179398	31.0	31.0	34.0	28.0	34.0
3	31.34457115651263	31.0	31.0	34.0	28.0	34.0
4	35.288326888501174	37.0	35.0	37.0	33.0	37.0
5	35.15114815209159	37.0	35.0	37.0	32.0	37.0
6	35.19559027080394	37.0	35.0	37.0	32.0	37.0
7	35.039577944155006	37.0	35.0	37.0	32.0	37.0
8	35.092271706541545	37.0	35.0	37.0	32.0	37.0
9	36.611500335147404	39.0	35.0	39.0	32.0	39.0
10	36.453710638328765	38.0	35.0	39.0	32.0	39.0
11	36.481937732758055	38.0	35.0	39.0	32.0	39.0
12	36.313504383534465	38.0	35.0	39.0	32.0	39.0
13	36.36997188149575	38.0	35.0	39.0	32.0	39.0
14	37.3214260219576	39.0	36.0	41.0	32.0	41.0
15	37.384437968692154	39.0	36.0	41.0	32.0	41.0
16	37.36314794156578	39.0	36.0	41.0	32.0	41.0
17	37.43474637689927	39.0	36.0	41.0	32.0	41.0
18	37.44033831739064	39.0	36.0	41.0	32.0	41.0
19	37.52915207706696	39.0	36.0	41.0	32.0	41.0
20	37.492958274753114	39.0	36.0	41.0	32.0	41.0
21	37.36685815642309	39.0	36.0	41.0	31.0	41.0
22	37.31944538549211	39.0	36.0	41.0	31.0	41.0
23	37.209416916111515	39.0	36.0	41.0	31.0	41.0
24	37.1270166315387	39.0	36.0	41.0	31.0	41.0
25	37.03867322765099	39.0	36.0	40.0	31.0	41.0
26	36.79532656908271	39.0	35.0	40.0	30.0	41.0
27	36.64302113001541	38.0	35.0	40.0	30.0	41.0
28	36.509643352345186	38.0	35.0	40.0	30.0	41.0
29	36.3792679872523	38.0	35.0	40.0	30.0	41.0
30	36.2143134559868	38.0	35.0	40.0	30.0	41.0
31	35.985464947453245	38.0	35.0	40.0	29.0	41.0
32	35.69410055874033	38.0	34.0	40.0	28.0	41.0
33	35.87253479120438	38.0	34.0	40.0	29.0	41.0
34	35.872214162821145	38.0	35.0	40.0	29.0	41.0
35	35.79877907131501	38.0	35.0	40.0	29.0	41.0
36	35.730781655700774	38.0	35.0	40.0	28.0	41.0
37	35.6089474072658	38.0	34.0	40.0	27.0	41.0
38	35.499072597525476	38.0	34.0	40.0	27.0	41.0
39	35.46514860218124	38.0	34.0	40.0	26.0	41.0
40	35.33533252188138	38.0	34.0	40.0	26.0	41.0
41	35.21846861834575	38.0	34.0	40.0	25.0	41.0
42	35.02909914313577	38.0	34.0	40.0	24.0	41.0
43	34.72818426331697	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	6.0
14	5.0
15	6.0
16	28.0
17	81.0
18	188.0
19	496.0
20	1057.0
21	2102.0
22	3912.0
23	6837.0
24	11359.0
25	18333.0
26	27697.0
27	40761.0
28	56885.0
29	77898.0
30	100410.0
31	124463.0
32	152455.0
33	187071.0
34	228115.0
35	277492.0
36	347779.0
37	454463.0
38	585856.0
39	600252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80216986770752	22.48993347868487	13.731212991620104	22.97668366198751
2	17.516715023073147	22.913223440475644	36.65544668978418	22.91461484666704
3	19.695233647347496	26.200208831920552	31.8414232512444	22.26313426948755
4	13.1139731059332	15.932568826209737	35.97925352872709	34.97420453912997
5	11.61264582541845	40.01626735325504	34.97698735151276	13.394099469813744
6	31.656880443120528	36.57066165599115	15.742369649438235	16.030088251450085
7	24.925227041192883	34.59704271737999	22.00164064938742	18.476089592039706
8	27.116661544678657	34.14967537888596	20.446078775368964	18.287584301066424
9	25.374318513445825	13.825556380988793	23.30578147421301	37.49434363135237
10	16.088497063527978	27.577156498108895	34.241508187518	22.09283825084513
11	31.95179805977481	22.05654069802614	24.90284355028784	21.08881769191121
12	20.057483224481004	27.073255721099283	32.0520095535159	20.817251500903808
13	30.65437228222073	21.05639187805958	28.359217521554697	19.93001831816499
14	20.880106763202026	22.415614239287986	28.38117754101018	28.323101456499806
15	22.999218392695965	28.988919567042792	26.619203583294414	21.39265845696683
16	21.883915586411167	28.519955184621455	26.97011017517199	22.626019053795392
17	19.672517428874944	27.282783344746896	28.177911245223846	24.86678798115431
18	20.625812157744324	26.69551918809634	31.800104536952116	20.87856411720722
19	20.176508949766607	25.332697319546714	32.94042240672134	21.55037132396534
20	23.855054192246357	24.45834976805864	31.754248628557463	19.932347411137542
21	23.378376579850986	25.335722115614967	30.55791153560427	20.727989768929778
22	22.015947934790237	24.911161739475524	31.46468490094398	21.60820542479026
23	21.093748109502457	26.51061340444427	32.26186990473102	20.133768581322247
24	20.08891085563011	25.870082589031846	31.01005805188614	23.0309485034519
25	21.04365748661225	26.619506062901237	31.894508422242176	20.442328028244336
26	22.172027411911888	25.782242511209898	30.763597668245207	21.282132408633007
27	21.03899930066715	26.903352925945732	30.489369656697747	21.568278116689374
28	20.825932665619685	26.419234315222468	30.67058518914655	22.0842478300113
29	20.02938286900697	26.462125923470243	31.449893648170242	22.05859755935255
30	19.59583884854483	26.682724300727646	32.88016846904181	20.84126838168571
31	20.82396654817532	26.124104962843404	32.0083012503297	21.04362723865157
32	21.67075820748165	26.24757713834933	30.419920338970748	21.66174431519827
33	22.346830376696005	26.120233223876042	30.068106308272696	21.464830091155253
34	20.923936058230954	28.222436243348476	30.136285211651032	20.717342486769542
35	19.90343036072508	28.982174271810596	30.346327050630244	20.76806831683408
36	20.660143593118953	26.010433126598603	32.004066535834156	21.32535674444829
37	20.78304105737191	26.14412911281521	31.32636097674295	21.746468853069924
38	20.920185311106327	25.780306641726213	31.92133836336754	21.378169683799918
39	21.623117669406728	25.34685336514612	31.96298980522733	21.06703916021982
40	20.172364979153105	25.995127658493267	31.271581919946957	22.560925442406674
41	20.906301497153063	25.56996232314017	31.15310065795364	22.370635521753123
42	18.867135227742946	27.068234559625992	31.522549249729582	22.54208096290148
43	19.460751456136826	26.822106903552562	31.08020307270884	22.63693856760177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	396.0
2	514.0
3	1430.5
4	2347.0
5	2347.0
6	4000.0
7	5653.0
8	6039.0
9	6425.0
10	9676.5
11	12928.0
12	12928.0
13	23265.5
14	33603.0
15	53256.0
16	72909.0
17	72628.5
18	72348.0
19	72348.0
20	77939.0
21	83530.0
22	70359.0
23	57188.0
24	63480.0
25	69772.0
26	69772.0
27	77341.0
28	84910.0
29	90549.0
30	96188.0
31	103911.0
32	111634.0
33	111634.0
34	122067.0
35	132500.0
36	142814.5
37	153129.0
38	159633.5
39	166138.0
40	166138.0
41	173716.5
42	181295.0
43	200025.0
44	218755.0
45	265014.5
46	311274.0
47	311274.0
48	312911.5
49	314549.0
50	288901.0
51	263253.0
52	245677.0
53	228101.0
54	228101.0
55	201900.0
56	175699.0
57	149408.0
58	123117.0
59	108840.0
60	94563.0
61	94563.0
62	83818.0
63	73073.0
64	63389.5
65	53706.0
66	45771.5
67	37837.0
68	37837.0
69	32229.5
70	26622.0
71	22323.0
72	18024.0
73	14352.0
74	10680.0
75	10680.0
76	8360.0
77	6040.0
78	4721.0
79	3402.0
80	2659.0
81	1916.0
82	1916.0
83	1424.5
84	933.0
85	745.0
86	557.0
87	461.0
88	365.0
89	365.0
90	280.5
91	196.0
92	117.5
93	39.0
94	27.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3306008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.23782488596435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60460200366452	31.392650673495837
2	14.061601684838152	12.159861425304266
3	5.390029164709883	6.991594114618999
4	2.6842088954858285	4.6423741672145615
5	1.4347327433282002	3.101736155709198
6	0.8874602010715681	2.3023109260317103
7	0.5578578430454282	1.6884391810202004
8	0.39359971885410533	1.3614716575182884
9	0.28500383171174	1.1090651190642957
>10	1.4699717616191499	11.86027418221399
>50	0.13547995774669946	4.10768298133456
>100	0.08322351058116324	6.74795846681881
>500	0.0059035023040190865	1.7124848710911518
>1k	0.005270984199704876	4.530647579245103
>5k	7.027978932939835E-4	2.337129339266782
>10k+	3.5139894664699175E-4	3.9543191600523504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35176	1.0640022649672958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34026	1.0292171101824317	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30606	0.9257690846483131	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17893	0.5412267604918076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12429	0.375951903322678	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9573	0.28956372761348426	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9405	0.2844820702188259	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9290	0.2810035547403394	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9052	0.2738045400979066	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7373	0.22301821411200456	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7217	0.21829953224553603	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6796	0.20556514079820737	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6778	0.20502067750592254	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5762	0.1742887494525119	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5665	0.1713546972663103	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4965	0.15018112478856677	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4962	0.1500903809065193	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4166	0.12601300420325662	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3838	0.11609167309939963	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3760	0.11373233216616536	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3556	0.10756174818693724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.0247960682490786E-5	0.0
6	0.0	0.0	0.0	3.0247960682490786E-5	0.0
7	0.0	0.0	0.0	3.0247960682490786E-5	0.0
8	0.0	0.0	0.0	3.0247960682490786E-5	0.0
9	0.0	0.0	0.0	1.2099184272996315E-4	0.0
10	0.0	0.0	0.0	1.5123980341245392E-4	0.0
11	0.0	0.0	0.0	4.23471449554871E-4	0.0
12	0.0	3.0247960682490786E-5	0.0	8.46942899109742E-4	0.0
13	0.0	3.0247960682490786E-5	0.0	9.98182702522196E-4	0.0
14	0.0	6.049592136498157E-5	0.0	0.001119174545252159	0.0
15	0.0	6.049592136498157E-5	0.0	0.0017241337589019748	0.0
16	0.0	6.049592136498157E-5	0.0	0.0027525644221066616	0.0
17	0.0	6.049592136498157E-5	0.0	0.004567442063056109	0.0
18	0.0	6.049592136498157E-5	0.0	0.005293393119435888	0.0
19	0.0	6.049592136498157E-5	0.0	0.006019344175815666	0.0
20	0.0	6.049592136498157E-5	0.0	0.007561990170622697	0.0
21	0.0	6.049592136498157E-5	0.0	0.010556538278189285	0.0
22	0.0	6.049592136498157E-5	0.0	0.017483321274479675	0.0
23	3.0247960682490786E-5	6.049592136498157E-5	0.0	0.030278208643173276	0.0
24	3.0247960682490786E-5	6.049592136498157E-5	0.0	0.045916404316021016	0.0
25	3.0247960682490786E-5	6.049592136498157E-5	0.0	0.05260120362685148	0.0
26	3.0247960682490786E-5	6.049592136498157E-5	0.0	0.06125212038204384	0.0
27	3.0247960682490786E-5	6.049592136498157E-5	0.0	0.1339682178627517	0.0
28	6.049592136498157E-5	6.049592136498157E-5	0.0	0.2127641554406402	0.0
29	6.049592136498157E-5	6.049592136498157E-5	0.0	0.30453646815131724	0.0
30	9.074388204747236E-5	6.049592136498157E-5	0.0	0.48073083912682607	0.0
31	9.074388204747236E-5	6.049592136498157E-5	0.0	0.7489697544591544	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7950	0.0	29.08805	1
GTATTGG	1060	0.0	22.863209	1
ATTGGAC	1140	0.0	20.122808	3
TTGGACC	1720	0.0	18.5	4
ACGTTTA	180	0.0	18.5	26
GGACCCT	1775	0.0	18.030985	6
TGGACCC	1865	0.0	16.664879	5
GTATCAA	13920	0.0	16.652657	2
TATACCG	290	0.0	16.586206	5
GACCCTC	1890	0.0	15.759259	7
CGCCGTT	95	7.066084E-5	15.578948	25
ATCACGT	215	0.0	15.488372	23
CGTCTGT	835	0.0	15.287425	34
TATTGGA	1490	0.0	15.2718115	2
TCACGTT	245	0.0	14.346938	24
CGTCGAG	130	4.44975E-6	14.230769	10
TGACGTA	105	1.6571152E-4	14.095238	20
ACCGTTA	105	1.6571152E-4	14.095238	8
CGCCGGA	250	0.0	14.059999	14
GCGTTAT	185	4.9076334E-9	14.0	1
>>END_MODULE