FastQCFastQC Report
Wed 25 May 2016
SRR2088197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634901
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235931.4430843213136453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224411.3726213391514226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT185661.1356039295345712No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG84120.5145265676637302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81680.4996021165807593No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT61630.37696472141126586No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA51840.317083419730002No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT50100.3064405734659163No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45210.27653050551684777No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA40580.2482107479290795No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT39460.2413601802188634No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37830.23139015756917392No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35800.21897350359440723No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34600.2116336096191757No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32920.20135775805385156No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT29580.18092838648945717No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29100.17799242889936454No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29000.17738077106809524No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.17193701636979852No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26860.16429129347893237No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22850.13976381444503366No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC20840.12746949203652086No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG20270.12398304239828589No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA19700.12049659276005091No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11780529830246601No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18410.11260620673667703No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC18340.11217804625478851No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG17410.10648962842398407No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA16720.10226918938822595No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16440.10055654746067193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.031.1884821
CCGTTAT250.005497014629.59999823
CGTGTCT1900.020.44736935
GTATTGG3450.019.3043481
TTGGACC7850.019.0891724
ATTGGAC3850.018.740263
ACGCATA500.007036123418.4999987
GTATCAA81300.018.408982
GGACCCT7750.018.1419376
ACCGTGA1054.800331E-717.6190478
ATCTCGT1301.3942554E-817.07692337
TGGACCC8500.016.7588235
TCGTGTC2350.016.53191634
GACCCTC8600.016.3488397
CGATAAG700.002593122215.85714223
TTTTTAC13000.015.6538461
GTCCTAA1455.349466E-815.3103451
CGAACCT3650.015.2054832
CGTAGAG1601.0972144E-815.0312528
GCTCGAC750.004105392414.829
Produced by FastQC (version 0.11.5)