##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1634901
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.108180862327444	31.0	31.0	33.0	30.0	34.0
2	31.27118339275589	31.0	31.0	34.0	28.0	34.0
3	31.308007029171797	31.0	31.0	34.0	28.0	34.0
4	35.260961367079716	37.0	35.0	37.0	33.0	37.0
5	35.121294806229855	37.0	35.0	37.0	32.0	37.0
6	35.177422975458455	37.0	35.0	37.0	32.0	37.0
7	35.01551164260099	37.0	35.0	37.0	32.0	37.0
8	35.07514583451842	37.0	35.0	37.0	32.0	37.0
9	36.55182974381935	39.0	35.0	39.0	32.0	39.0
10	36.4195691359905	38.0	35.0	39.0	32.0	39.0
11	36.45884429699413	38.0	35.0	39.0	32.0	39.0
12	36.299172855114776	38.0	35.0	39.0	32.0	39.0
13	36.361230435359694	38.0	35.0	39.0	32.0	39.0
14	37.27916675076962	39.0	36.0	41.0	32.0	41.0
15	37.34934959364512	39.0	36.0	41.0	32.0	41.0
16	37.31184334708952	39.0	36.0	41.0	32.0	41.0
17	37.414727864256	39.0	36.0	41.0	32.0	41.0
18	37.440429114668106	39.0	36.0	41.0	32.0	41.0
19	37.538795927092835	39.0	36.0	41.0	32.0	41.0
20	37.50396813018036	39.0	36.0	41.0	32.0	41.0
21	37.38822044882228	39.0	36.0	41.0	32.0	41.0
22	37.3475396981224	39.0	36.0	41.0	32.0	41.0
23	37.23805845124567	39.0	36.0	41.0	31.0	41.0
24	37.146558109634775	39.0	36.0	41.0	31.0	41.0
25	37.05547981192745	39.0	36.0	40.0	31.0	41.0
26	36.78826730181216	39.0	35.0	40.0	31.0	41.0
27	36.61955800381797	38.0	35.0	40.0	30.0	41.0
28	36.461481153904735	38.0	35.0	40.0	30.0	41.0
29	36.31176688986061	38.0	35.0	40.0	30.0	41.0
30	36.12052228238896	38.0	35.0	40.0	30.0	41.0
31	35.843307943416754	38.0	34.0	40.0	29.0	41.0
32	35.537239257912255	38.0	34.0	40.0	28.0	41.0
33	35.65306217318358	38.0	34.0	40.0	29.0	41.0
34	35.608050885038296	38.0	34.0	40.0	28.0	41.0
35	35.5072747524162	38.0	34.0	40.0	27.0	41.0
36	35.412981581147726	38.0	34.0	40.0	27.0	41.0
37	35.251004800902315	38.0	34.0	40.0	26.0	41.0
38	35.111119878206694	38.0	34.0	40.0	25.0	41.0
39	35.00902990456303	38.0	34.0	40.0	25.0	41.0
40	34.81720116386252	38.0	33.0	40.0	24.0	41.0
41	34.648316320070755	38.0	33.0	40.0	23.0	41.0
42	34.405020854473754	38.0	33.0	40.0	22.0	41.0
43	34.06839557869253	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	3.0
16	19.0
17	28.0
18	123.0
19	287.0
20	595.0
21	1143.0
22	2204.0
23	3788.0
24	6100.0
25	9760.0
26	15002.0
27	21895.0
28	30252.0
29	41022.0
30	52356.0
31	63511.0
32	78193.0
33	95151.0
34	116409.0
35	138703.0
36	173613.0
37	226530.0
38	275437.0
39	282774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.921010507669884	23.386492515448946	14.728353582265838	21.96414339461533
2	17.315849705884332	23.54362741230203	36.1876346029515	22.952888278862147
3	19.97240199865313	25.790246626554147	32.843273078920376	21.394078295872347
4	13.366497420944754	16.290772346460123	35.09460205847327	35.24812817412186
5	11.124037479945269	40.86204608107769	34.513098958285546	13.50081748069149
6	31.35039981014141	35.872508488281554	15.562287869418393	17.214803832158644
7	23.852942777574913	36.131423248257846	21.588585486215987	18.427048487951257
8	27.381474474601212	33.864802822923224	20.493167476195808	18.260555226279756
9	25.011973202047095	14.092902261360168	24.841259501339835	36.0538650352529
10	16.47524834837094	28.665160765086085	34.01484248893358	20.844748397609397
11	30.701308519598435	22.41279441385136	25.211312489257757	21.674584577292446
12	19.77398019818937	28.51885221184647	31.70136907372373	20.005798516240432
13	30.372114274809302	21.781991692463336	29.387467498031988	18.458426534695374
14	20.561856650647346	23.586810455189642	29.366426468636327	26.484906425526685
15	22.813430293332747	29.551881123077177	27.386673566167	20.248015017423075
16	21.287955662147127	28.625280674487325	28.332847065357473	21.753916598008075
17	18.709573240214546	28.045857210925924	28.919059930845965	24.32550961801357
18	19.49029329604667	27.121458730528637	33.463983446092456	19.924264527332237
19	18.978152193924892	25.948176678587874	35.0229157606485	20.050755366838725
20	22.94499789283877	24.8718423929033	33.74406156703066	18.439098147227263
21	22.415302210959563	25.62442618849704	32.34850305920665	19.611768541336755
22	20.63990418991731	25.643876907531403	33.1127083535945	20.603510548956788
23	19.922490719621557	27.545215276032003	33.84963370870774	18.682660295638694
24	18.65226090142461	26.759479625983467	32.52612849340725	22.062130979184673
25	19.60877141796353	27.682838288067597	33.64870411113578	19.059686182833087
26	20.933010622661556	26.662715357076667	32.36379450498838	20.0404795152734
27	19.667490569765388	27.890373790217264	31.875691555635477	20.566444084381867
28	19.450719034363548	27.691095668789735	32.0563141132093	20.80187118363742
29	18.698808062384206	27.399457214840535	32.892695031686934	21.00903969108833
30	18.029715560758724	27.525336396515755	35.08603884883549	19.358909193890028
31	19.57243894278614	26.81581331224337	33.76932303546209	19.842424709508403
32	20.27278715958948	27.430345935319632	31.766999959018925	20.529866946071966
33	21.177612589386147	27.368935489060192	31.247029636656897	20.20642228489676
34	19.714710554339376	29.606318670060144	31.299509878579805	19.379460897020675
35	18.1961476566471	30.37015696974924	31.59402312433597	19.839672249267693
36	19.29450162425737	27.039129586439792	33.494811000788424	20.17155778851441
37	19.15094553125847	27.129899608600155	32.92205460758786	20.79710025255352
38	19.502954613153946	26.815690980677115	33.38251062296738	20.29884378320155
39	20.53983696872165	26.217611953262004	33.247211910690616	19.995339167325728
40	18.884140385258803	26.772079777307617	32.65690093773262	21.68687889970096
41	19.583265286399605	26.287402111809826	32.453280045703075	21.676052556087495
42	17.182324801318245	27.881565917446988	32.802720164707225	22.133389116527542
43	17.723336153075937	27.565277652897635	32.58704961340167	22.12433658062476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	301.0
2	380.0
3	1031.0
4	1682.0
5	1682.0
6	2677.5
7	3673.0
8	4086.5
9	4500.0
10	6743.5
11	8987.0
12	8987.0
13	16140.5
14	23294.0
15	36434.0
16	49574.0
17	48078.5
18	46583.0
19	46583.0
20	51007.0
21	55431.0
22	45083.5
23	34736.0
24	37895.5
25	41055.0
26	41055.0
27	44354.5
28	47654.0
29	49398.5
30	51143.0
31	53662.0
32	56181.0
33	56181.0
34	60296.5
35	64412.0
36	69314.5
37	74217.0
38	75854.0
39	77491.0
40	77491.0
41	81194.5
42	84898.0
43	95139.0
44	105380.0
45	131791.0
46	158202.0
47	158202.0
48	160000.0
49	161798.0
50	146444.0
51	131090.0
52	119157.5
53	107225.0
54	107225.0
55	93213.5
56	79202.0
57	64112.5
58	49023.0
59	42728.5
60	36434.0
61	36434.0
62	31511.5
63	26589.0
64	22835.0
65	19081.0
66	15834.0
67	12587.0
68	12587.0
69	10701.0
70	8815.0
71	7173.0
72	5531.0
73	4475.0
74	3419.0
75	3419.0
76	2745.0
77	2071.0
78	1594.0
79	1117.0
80	916.5
81	716.0
82	716.0
83	517.0
84	318.0
85	213.5
86	109.0
87	84.0
88	59.0
89	59.0
90	38.5
91	18.0
92	9.5
93	1.0
94	2.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1634901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.92642109154002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.24370104256494	26.261657636770263
2	14.260599775915153	10.817070242385588
3	6.079177934811484	6.916843867381769
4	3.1520558931946505	4.781847964374824
5	1.9193342671978526	3.6396739816584076
6	1.2498465326054862	2.8441323537238126
7	0.8393083174698656	2.2282372471795853
8	0.5808007104636437	1.7622153852247815
9	0.40330294987464393	1.3766253753965405
>10	2.014948798293258	13.537348863764567
>50	0.14547580854371858	3.84414120945374
>100	0.09065307769708114	6.8947044733903216
>500	0.010397445684171756	2.7221022233811674
>1k	0.009097764973650287	6.363627914411983
>5k	8.123004440759184E-4	2.02942305012575
>10k+	4.87380266445551E-4	3.9803482113769757
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23593	1.4430843213136453	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22441	1.3726213391514226	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18566	1.1356039295345712	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8412	0.5145265676637302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8168	0.4996021165807593	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6163	0.37696472141126586	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5184	0.317083419730002	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5010	0.3064405734659163	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4521	0.27653050551684777	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4058	0.2482107479290795	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3946	0.2413601802188634	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3783	0.23139015756917392	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3580	0.21897350359440723	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3460	0.2116336096191757	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3292	0.20135775805385156	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2958	0.18092838648945717	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2910	0.17799242889936454	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2900	0.17738077106809524	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.17193701636979852	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2686	0.16429129347893237	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2285	0.13976381444503366	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2084	0.12746949203652086	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2027	0.12398304239828589	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1970	0.12049659276005091	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1926	0.11780529830246601	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1841	0.11260620673667703	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1834	0.11217804625478851	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1741	0.10648962842398407	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1672	0.10226918938822595	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1644	0.10055654746067193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0	0.0
3	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0	0.0
4	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0	0.0
5	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0	0.0
6	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0
7	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0
8	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0
9	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	0.0	0.0
10	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	6.11657831269294E-5	0.0
11	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	1.223315662538588E-4	0.0
12	1.223315662538588E-4	6.11657831269294E-5	6.11657831269294E-5	1.8349734938078818E-4	0.0
13	1.223315662538588E-4	6.11657831269294E-5	1.8349734938078818E-4	1.8349734938078818E-4	0.0
14	1.8349734938078818E-4	6.11657831269294E-5	1.8349734938078818E-4	2.446631325077176E-4	0.0
15	1.8349734938078818E-4	6.11657831269294E-5	1.8349734938078818E-4	4.281604818885058E-4	0.0
16	1.8349734938078818E-4	6.11657831269294E-5	1.8349734938078818E-4	8.563209637770116E-4	0.0
17	1.8349734938078818E-4	6.11657831269294E-5	2.446631325077176E-4	0.001406813011919376	0.0
18	1.8349734938078818E-4	6.11657831269294E-5	4.281604818885058E-4	0.0017126419275540232	0.0
19	1.8349734938078818E-4	6.11657831269294E-5	4.281604818885058E-4	0.0020796366263155994	0.0
20	1.8349734938078818E-4	6.11657831269294E-5	4.893262650154352E-4	0.002813626023838752	0.0
21	1.8349734938078818E-4	6.11657831269294E-5	6.728236143962234E-4	0.003853444336996552	0.0
22	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.006850567710216093	6.11657831269294E-5
23	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.011560333010989656	6.11657831269294E-5
24	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.021102195178790643	6.11657831269294E-5
25	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.028013928672133665	6.11657831269294E-5
26	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.04079757734566191	6.11657831269294E-5
27	1.8349734938078818E-4	6.11657831269294E-5	7.339893975231527E-4	0.13701135420432184	6.11657831269294E-5
28	1.8349734938078818E-4	6.11657831269294E-5	8.563209637770116E-4	0.23683391226747064	6.11657831269294E-5
29	1.8349734938078818E-4	6.11657831269294E-5	8.563209637770116E-4	0.34674882454656275	6.11657831269294E-5
30	1.8349734938078818E-4	6.11657831269294E-5	8.563209637770116E-4	0.5531221768168225	6.11657831269294E-5
31	1.8349734938078818E-4	6.11657831269294E-5	8.563209637770116E-4	0.852528685223142	6.11657831269294E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4775	0.0	31.188482	1
CCGTTAT	25	0.0054970146	29.599998	23
CGTGTCT	190	0.0	20.447369	35
GTATTGG	345	0.0	19.304348	1
TTGGACC	785	0.0	19.089172	4
ATTGGAC	385	0.0	18.74026	3
ACGCATA	50	0.0070361234	18.499998	7
GTATCAA	8130	0.0	18.40898	2
GGACCCT	775	0.0	18.141937	6
ACCGTGA	105	4.800331E-7	17.619047	8
ATCTCGT	130	1.3942554E-8	17.076923	37
TGGACCC	850	0.0	16.758823	5
TCGTGTC	235	0.0	16.531916	34
GACCCTC	860	0.0	16.348839	7
CGATAAG	70	0.0025931222	15.857142	23
TTTTTAC	1300	0.0	15.653846	1
GTCCTAA	145	5.349466E-8	15.310345	1
CGAACCT	365	0.0	15.20548	32
CGTAGAG	160	1.0972144E-8	15.03125	28
GCTCGAC	75	0.0041053924	14.8	29
>>END_MODULE