FastQCFastQC Report
Wed 25 May 2016
SRR2088196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473227
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204051.385054713224778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT193421.312900184425075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT161901.0989480915025316No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG71650.4863473178267843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69260.47012442753221334No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT50960.3459073177453305No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43820.2974422814678254No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT42530.2886859934008812No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40060.2719200774897555No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA35450.2406282263357921No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34550.23451918814955197No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG32210.21863568886532758No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT31560.21422360573082086No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28330.19229894646242568No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27500.1866650556906709No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.17845179324028135No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25690.1743791011161213No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA24900.16901672315264382No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.1578846980132729No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22250.15102899960427008No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20140.13670669896764043No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17740.12041593047100005No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16580.11254205903095721No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC16520.1121347898185412No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16150.10962329634197582No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.10935178353369847No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA15940.10819785409851977No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14790.10039186086054626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43900.031.0159441
CTAACGC250.005496839529.63
TATACCG851.9772415E-923.9411775
TTACGCT400.00193144423.1254
GTATTGG2800.018.51
GTTATCA1700.018.51
GTATCAA74950.018.2655092
GCCGATC852.7239592E-517.4117648
ACGGTGT852.7239592E-517.41176429
TATAGTG1603.45608E-1117.343755
ATTGGAC3600.016.9583343
CCGATAC803.3833747E-416.18753
GCCGATA803.3833747E-416.18752
TTGGACC6050.015.9008264
ACCGACC1403.4748155E-815.8571438
TACCGAC2500.015.547
AAGTACG750.004105135314.835
CCGACCG1508.105053E-814.89
AGGGGTA1001.09369576E-414.85
CCGCCGG1405.9976264E-714.53571513
Produced by FastQC (version 0.11.5)