##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1473227
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092673430503243	31.0	31.0	33.0	30.0	34.0
2	31.246649701641363	31.0	31.0	34.0	28.0	34.0
3	31.297032975909346	31.0	31.0	34.0	28.0	34.0
4	35.24377981125787	37.0	35.0	37.0	33.0	37.0
5	35.100910450324356	35.0	35.0	37.0	32.0	37.0
6	35.15912754789316	37.0	35.0	37.0	32.0	37.0
7	34.99417876539054	37.0	35.0	37.0	32.0	37.0
8	35.056778758466955	37.0	35.0	37.0	32.0	37.0
9	36.54730126450303	39.0	35.0	39.0	32.0	39.0
10	36.401646860938605	38.0	35.0	39.0	32.0	39.0
11	36.429607249935	38.0	35.0	39.0	32.0	39.0
12	36.28035937435303	38.0	35.0	39.0	32.0	39.0
13	36.33791194432359	38.0	35.0	39.0	32.0	39.0
14	37.26087629401307	39.0	36.0	41.0	32.0	41.0
15	37.335671963655294	39.0	36.0	41.0	32.0	41.0
16	37.299534287655604	39.0	36.0	41.0	32.0	41.0
17	37.38827689147701	39.0	36.0	41.0	32.0	41.0
18	37.41903929265483	39.0	36.0	41.0	32.0	41.0
19	37.51429752509287	39.0	36.0	41.0	32.0	41.0
20	37.47574677900962	39.0	36.0	41.0	32.0	41.0
21	37.35610533882423	39.0	36.0	41.0	31.0	41.0
22	37.31912800946494	39.0	36.0	41.0	31.0	41.0
23	37.214845370061774	39.0	36.0	41.0	31.0	41.0
24	37.12100986473911	39.0	36.0	41.0	31.0	41.0
25	37.03552609339905	39.0	36.0	40.0	31.0	41.0
26	36.77117579300406	39.0	35.0	40.0	31.0	41.0
27	36.590939481831384	38.0	35.0	40.0	30.0	41.0
28	36.43720214196455	38.0	35.0	40.0	30.0	41.0
29	36.2829658973125	38.0	35.0	40.0	30.0	41.0
30	36.108418458255244	38.0	35.0	40.0	30.0	41.0
31	35.84132927240677	38.0	34.0	40.0	29.0	41.0
32	35.53873367783783	38.0	34.0	40.0	28.0	41.0
33	35.659645797965965	38.0	34.0	40.0	29.0	41.0
34	35.633215383644206	38.0	34.0	40.0	28.0	41.0
35	35.53027537507798	38.0	34.0	40.0	27.0	41.0
36	35.43885158227483	38.0	34.0	40.0	27.0	41.0
37	35.281484794943346	38.0	34.0	40.0	26.0	41.0
38	35.14030560124136	38.0	34.0	40.0	25.0	41.0
39	35.051012505201165	38.0	34.0	40.0	25.0	41.0
40	34.8660498348184	38.0	34.0	40.0	24.0	41.0
41	34.709664566288836	38.0	33.0	40.0	23.0	41.0
42	34.481033133386774	38.0	33.0	40.0	22.0	41.0
43	34.150771062436405	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	4.0
16	13.0
17	53.0
18	122.0
19	262.0
20	559.0
21	1085.0
22	2028.0
23	3473.0
24	5745.0
25	9059.0
26	13746.0
27	20217.0
28	27443.0
29	36810.0
30	47125.0
31	57530.0
32	70721.0
33	85540.0
34	103218.0
35	124878.0
36	155298.0
37	201679.0
38	247716.0
39	258897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.835748326632626	23.423477848288147	14.63854518007069	22.10222864500854
2	17.38123181288423	23.654127978919746	36.319318068430725	22.64532213976529
3	20.07558916582441	25.842996361049586	32.57468129487173	21.506733178254265
4	13.440562791748997	16.32029551454053	35.33732411909366	34.901817574616814
5	11.161077009856593	40.825955538420075	34.60294985090553	13.410017600817797
6	31.328912652293234	36.262843404309045	15.48037064213458	16.927873301263148
7	23.881927225064434	35.776631842886395	21.81259235677869	18.52884857527048
8	27.135465206651794	34.080762842386136	20.588816251670654	18.19495569929142
9	24.991328559685645	14.18118185452751	24.660082933587287	36.16740665219956
10	16.373851415973235	28.54577061104636	33.998087192265686	21.082290780714715
11	30.84528046254922	22.400824855911548	25.211457568996497	21.542437112542736
12	19.671238716097385	28.28986978924497	31.9235257024206	20.115365792237043
13	30.39117529070537	21.770304236889494	29.233444676210794	18.60507579619434
14	20.36047397990941	23.675645368975726	29.253197233012973	26.710683418101894
15	22.679736388214444	29.714225981467894	27.34907790856399	20.256959721753674
16	21.095594908320304	28.721575154405944	28.17325503808985	22.0095748991839
17	18.60894485371229	28.038584685184293	28.983992283605986	24.368478177497426
18	19.45518239891069	27.24271276592134	33.356706060912536	19.945398774255427
19	18.962997555705943	26.023009352937464	34.75560792735946	20.25838516399713
20	23.029716398083934	24.9622088109979	33.4132486032363	18.59482618768187
21	22.46693822472708	25.728689468764827	32.01577217903283	19.788600127475263
22	20.680994850080808	25.65789250400651	32.88298408867065	20.778128557242027
23	20.014023636547524	27.478996787324693	33.635821227821644	18.871158348306132
24	18.738320706856445	26.79288392080786	32.31667624880619	22.152119123529506
25	19.716241964069354	27.603485409919852	33.371842899974006	19.30842972603679
26	20.997646662734258	26.532910406882305	32.162117582694314	20.30732534768912
27	19.911866942433175	27.71419475749494	31.762382850708004	20.61155544936388
28	19.661056985786985	27.51341103577385	31.82197991212488	21.00355206631429
29	18.68476480542374	27.29178870601747	32.83825235350697	21.18519413505183
30	18.32969393039905	27.370255907609618	34.684878840803215	19.615171321188114
31	19.58829155316866	26.734101397815813	33.574866602363386	20.102740446652145
32	20.290355797171785	27.308011596312042	31.763333145536976	20.638299460979198
33	21.25531231778945	27.12351864308759	31.31275764020073	20.308411398922228
34	19.779165057387626	29.277633385757934	31.37778495778315	19.565416599071288
35	18.419496791736776	29.899058325702693	31.631717311724532	20.049727570835994
36	19.346577275599756	26.71102280911224	33.576699313819255	20.36570060146875
37	19.202879121818974	26.750256409908317	32.956360425107604	21.090504043165108
38	19.616325250623294	26.455461378321193	33.45859124221861	20.4696221288369
39	20.537703965512442	25.825008637501213	33.41433465446941	20.222952742516938
40	18.97867742038396	26.42647738603759	32.86221335883744	21.732631834741014
41	19.503511678784058	25.96816376566544	32.62782992709202	21.900494628458482
42	17.27445940102917	27.48911063943303	32.93531818246611	22.301111777071693
43	17.767323026254612	27.232123766398526	32.67948523886678	22.32106796848008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	245.5
2	335.0
3	831.5
4	1328.0
5	1328.0
6	2304.5
7	3281.0
8	3645.0
9	4009.0
10	5971.0
11	7933.0
12	7933.0
13	14125.5
14	20318.0
15	32141.5
16	43965.0
17	42920.5
18	41876.0
19	41876.0
20	45334.0
21	48792.0
22	40100.5
23	31409.0
24	34029.5
25	36650.0
26	36650.0
27	39670.0
28	42690.0
29	43903.5
30	45117.0
31	48816.0
32	52515.0
33	52515.0
34	55879.0
35	59243.0
36	62936.0
37	66629.0
38	68445.0
39	70261.0
40	70261.0
41	73106.5
42	75952.0
43	85068.0
44	94184.0
45	117843.5
46	141503.0
47	141503.0
48	142840.0
49	144177.0
50	130215.5
51	116254.0
52	106006.5
53	95759.0
54	95759.0
55	83389.5
56	71020.0
57	58574.0
58	46128.0
59	40403.5
60	34679.0
61	34679.0
62	30052.0
63	25425.0
64	21469.5
65	17514.0
66	14976.0
67	12438.0
68	12438.0
69	10314.5
70	8191.0
71	6968.0
72	5745.0
73	4549.0
74	3353.0
75	3353.0
76	2739.0
77	2125.0
78	1627.0
79	1129.0
80	874.0
81	619.0
82	619.0
83	423.5
84	228.0
85	185.5
86	143.0
87	121.0
88	99.0
89	99.0
90	70.0
91	41.0
92	25.0
93	9.0
94	5.5
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1473227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.83959965611457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08214142613133	30.87970081050897
2	13.986032298936934	11.98312048927892
3	5.659382799297285	7.2733708026779
4	2.7625350640480084	4.733835847191819
5	1.53539042048463	3.288775546469748
6	0.9226037643402605	2.3714385543336616
7	0.6022522936421926	1.8060173006125797
8	0.40927095672607894	1.402640314961617
9	0.2977217908877378	1.147885409747893
>10	1.532290097965085	11.930947791773757
>50	0.12472722352038802	3.671500361239974
>100	0.06826631795607598	5.78217193593452
>500	0.009091524088551638	2.6836349264633848
>1k	0.007337019439883779	5.911786383263797
>5k	4.7850126781850727E-4	1.3110350944380131
>10k+	4.7850126781850727E-4	3.8221384311033066
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20405	1.385054713224778	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19342	1.312900184425075	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16190	1.0989480915025316	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7165	0.4863473178267843	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6926	0.47012442753221334	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5096	0.3459073177453305	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4382	0.2974422814678254	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4253	0.2886859934008812	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4006	0.2719200774897555	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3545	0.2406282263357921	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3455	0.23451918814955197	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3221	0.21863568886532758	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3156	0.21422360573082086	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2833	0.19229894646242568	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2750	0.1866650556906709	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2629	0.17845179324028135	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2569	0.1743791011161213	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2490	0.16901672315264382	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2326	0.1578846980132729	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2225	0.15102899960427008	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2014	0.13670669896764043	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1774	0.12041593047100005	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1658	0.11254205903095721	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1652	0.1121347898185412	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1615	0.10962329634197582	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1611	0.10935178353369847	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1594	0.10819785409851977	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1479	0.10039186086054626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.787820206933487E-5	0.0
13	0.0	0.0	0.0	2.715128082773395E-4	0.0
14	0.0	0.0	0.0	4.751474144853441E-4	0.0
15	0.0	0.0	0.0	0.001086051233109358	0.0
16	0.0	0.0	0.0	0.001561198647594702	0.0
17	0.0	0.0	0.0	0.00251149347656539	0.0
18	0.0	0.0	0.0	0.0028508844869120646	0.0
19	0.0	0.0	0.0	0.003936935720021423	0.0
20	0.0	0.0	0.0	0.004887230548992111	0.0
21	0.0	0.0	0.0	0.006312672792448143	0.0
22	0.0	0.0	0.0	0.01045324311867757	0.0
23	0.0	0.0	0.0	0.017648332538027067	0.0
24	0.0	0.0	0.0	0.028237332060843306	0.0
25	0.0	0.0	0.0	0.03685786372364883	0.0
26	0.0	0.0	0.0	0.05131592076441716	0.0
27	0.0	0.0	0.0	0.16141436452087832	0.0
28	0.0	0.0	0.0	0.27633216062426225	0.0
29	0.0	0.0	0.0	0.3949153796393903	0.0
30	0.0	0.0	0.0	0.6311315228406756	0.0
31	0.0	0.0	0.0	0.9776497444046301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4390	0.0	31.015944	1
CTAACGC	25	0.0054968395	29.6	3
TATACCG	85	1.9772415E-9	23.941177	5
TTACGCT	40	0.001931444	23.125	4
GTATTGG	280	0.0	18.5	1
GTTATCA	170	0.0	18.5	1
GTATCAA	7495	0.0	18.265509	2
GCCGATC	85	2.7239592E-5	17.411764	8
ACGGTGT	85	2.7239592E-5	17.411764	29
TATAGTG	160	3.45608E-11	17.34375	5
ATTGGAC	360	0.0	16.958334	3
CCGATAC	80	3.3833747E-4	16.1875	3
GCCGATA	80	3.3833747E-4	16.1875	2
TTGGACC	605	0.0	15.900826	4
ACCGACC	140	3.4748155E-8	15.857143	8
TACCGAC	250	0.0	15.54	7
AAGTACG	75	0.0041051353	14.8	35
CCGACCG	150	8.105053E-8	14.8	9
AGGGGTA	100	1.09369576E-4	14.8	5
CCGCCGG	140	5.9976264E-7	14.535715	13
>>END_MODULE