FastQCFastQC Report
Wed 25 May 2016
SRR2088195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791284
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121981.5415451342375177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118081.492258152572275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99571.2583345549764686No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG47520.600542915059574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.4995677910838586No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT33470.4229834041886352No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29610.37420193002765123No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT28870.36485004119886155No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27110.34260771101147No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA23480.29673290499997473No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT23160.2926888449659035No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT21220.2681717310093468No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG21010.2655178166119876No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18270.23089055257025293No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18190.22987953756173513No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT16800.2123131517887383No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15290.1932302435029648No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15000.18956531409708777No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14680.18552125406301656No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.17035602893524956No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13440.16985052143099066No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC11650.14722906061540483No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11000.1390145636711977No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG10580.13370673487647924No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA9850.12448122292375431No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9660.12208006227852453No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC9610.12144817789820089No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.12081629351787727No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC9230.11664585660774135No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC9150.11563484159922355No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.11512933409496465No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC9070.11462382659070573No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC9010.1138655653343174No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT8170.10324990774488046No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8160.10312353086881575No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA8140.1028707771166863No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA8020.1013542546039096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.032.4121
GCATCGG405.937827E-527.7520
AAGACCG358.8669435E-426.428575
ATTGGAC2150.024.0930253
TTGGACC4150.021.8433744
GACCCTC4000.021.2750027
GTATTGG2450.021.1428571
GGACCCT4200.021.1428576
TGGACCC4250.020.8941175
ACGTTTA555.1411474E-420.18181826
TCACGTT656.898657E-519.92307724
AGCGGGC759.259789E-619.73333426
CTAATAC1052.2568202E-819.380953
GTATCAA43150.018.7786792
AGCGTCG500.007032991418.533
GTATTAG902.1504911E-618.51
GTACACT801.6156335E-518.54
CCGTCAG609.2333485E-418.514
TCCACGG1002.8721115E-718.52
GCGTCGT500.007032991418.534
Produced by FastQC (version 0.11.5)