##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791284
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.044780382264776	31.0	31.0	33.0	28.0	34.0
2	31.193723113319617	31.0	31.0	34.0	28.0	34.0
3	31.235775018830154	31.0	31.0	34.0	28.0	34.0
4	35.2034958371457	37.0	35.0	37.0	33.0	37.0
5	35.07274884870666	35.0	35.0	37.0	32.0	37.0
6	35.13464571506564	37.0	35.0	37.0	32.0	37.0
7	34.95737181593461	36.0	35.0	37.0	32.0	37.0
8	35.0297920847635	37.0	35.0	37.0	32.0	37.0
9	36.48910631328322	38.0	35.0	39.0	32.0	39.0
10	36.349523053669735	38.0	35.0	39.0	32.0	39.0
11	36.39402540680717	38.0	35.0	39.0	32.0	39.0
12	36.217316412312144	38.0	35.0	39.0	32.0	39.0
13	36.27641150332877	38.0	35.0	39.0	32.0	39.0
14	37.17100055100318	39.0	36.0	41.0	31.0	41.0
15	37.232471527289825	39.0	36.0	41.0	32.0	41.0
16	37.20019487314289	39.0	36.0	41.0	32.0	41.0
17	37.32070407085193	39.0	36.0	41.0	32.0	41.0
18	37.3572345706472	39.0	36.0	41.0	32.0	41.0
19	37.46824654611998	39.0	36.0	41.0	32.0	41.0
20	37.429987210660144	39.0	36.0	41.0	32.0	41.0
21	37.302948119764835	39.0	36.0	41.0	31.0	41.0
22	37.266091567629324	39.0	36.0	41.0	31.0	41.0
23	37.146662892210635	39.0	36.0	40.0	31.0	41.0
24	37.061631474919245	39.0	36.0	40.0	31.0	41.0
25	36.979605299740676	39.0	35.0	40.0	31.0	41.0
26	36.69146223100682	38.0	35.0	40.0	30.0	41.0
27	36.51086209249776	38.0	35.0	40.0	30.0	41.0
28	36.34544866318541	38.0	35.0	40.0	30.0	41.0
29	36.19853048968512	38.0	35.0	40.0	30.0	41.0
30	36.002159780811944	38.0	35.0	40.0	30.0	41.0
31	35.70387370400513	38.0	34.0	40.0	29.0	41.0
32	35.39802649870337	38.0	34.0	40.0	27.0	41.0
33	35.50986118763933	38.0	34.0	40.0	28.0	41.0
34	35.46533482289544	38.0	34.0	40.0	27.0	41.0
35	35.355426117550714	38.0	34.0	40.0	27.0	41.0
36	35.24909008649233	38.0	34.0	40.0	26.0	41.0
37	35.089840057425654	38.0	34.0	40.0	25.0	41.0
38	34.933609171928154	38.0	34.0	40.0	25.0	41.0
39	34.83440458798611	38.0	33.0	40.0	24.0	41.0
40	34.62465562301272	38.0	33.0	40.0	23.0	41.0
41	34.45092154018026	38.0	33.0	40.0	22.0	41.0
42	34.195297769195385	38.0	33.0	40.0	21.0	41.0
43	33.849448491312856	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	6.0
17	26.0
18	66.0
19	125.0
20	308.0
21	602.0
22	1053.0
23	1858.0
24	3176.0
25	5081.0
26	7706.0
27	11060.0
28	15637.0
29	20609.0
30	26200.0
31	31890.0
32	38761.0
33	47865.0
34	57185.0
35	68539.0
36	84731.0
37	110646.0
38	131176.0
39	126974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.981725903721035	23.477790527800384	14.907289923718917	21.633193644759658
2	17.315780427760448	23.557407959721164	36.48184469798454	22.644966914533846
3	20.025806158092415	25.821196940668585	33.00028813927743	21.152708761961573
4	13.232417185233114	15.960135678214144	35.08184166493952	35.72560547161323
5	10.789046663397718	41.16600360932358	34.46866106227347	13.576288665005231
6	31.411983560895962	35.90000556058255	15.25722749354214	17.43078338497935
7	23.635256115377032	36.52961515713701	21.2400351833223	18.595093544163664
8	27.583143346762984	33.80644623169431	20.232811481086436	18.37759894045627
9	24.995576809337734	13.925088842943872	24.84518832682071	36.234146020897676
10	16.505199144681303	28.465380318570826	33.72809256853418	21.301327968213688
11	30.614798226679675	22.1381956415143	25.530530125719714	21.71647600608631
12	19.890582900703162	28.381466072863855	31.771273019547973	19.956678006885014
13	30.871216908215004	21.509723436844418	29.60744814756775	18.011611507372827
14	20.53864352116307	23.440509349361292	29.40195934708651	26.618887782389127
15	22.659879385909484	29.579645234833514	27.726075593592185	20.03439978566482
16	21.092047861450503	28.704990875589548	28.248643976119826	21.954317286840123
17	18.464040723684544	27.846386379605804	28.89013299902437	24.79943989768528
18	19.40516931973855	27.044272347222993	33.80619347794218	19.744364855096276
19	18.80500553530717	25.43536833804298	35.476769402641786	20.28285672400807
20	23.341935386030805	24.307201965413178	34.05705157693066	18.293811071625356
21	22.880280657766363	25.207889961126472	32.39936609358966	19.512463287517505
22	20.660344452813405	25.10779947528321	33.49568549345115	20.73617057845224
23	19.894121453232973	27.35098902543208	34.33697635741403	18.417913163920918
24	18.30101455356105	26.321902123637024	32.79100803251424	22.586075290287685
25	19.51612821692338	27.366786134940174	34.26658443744597	18.850501210690474
26	21.09520728335212	26.198305538845723	32.53130355220124	20.175183625600923
27	19.65602741872703	27.836149852644564	32.08355028030391	20.424272448324494
28	19.517771116312222	27.275794784173574	32.190085986826475	21.016348112687734
29	18.383033146127055	27.079784249397182	33.36084136669009	21.176341237785675
30	17.83190358960879	27.194534452863955	35.595058158638366	19.378503798888893
31	19.312155938954913	26.663246065887847	34.09976696104054	19.924831034116703
32	20.300675863533193	27.122373256630993	32.048291131881854	20.52865974795396
33	21.485964584144252	26.94911055954626	31.34614120846624	20.218783647843253
34	19.675868588269193	29.735973430525576	31.435489659844002	19.15266832136123
35	18.04484862577785	30.567533275031465	31.694562255776688	19.693055843413994
36	19.15898716516447	26.612316184833762	33.96808225618109	20.260614393820674
37	19.068501321902122	27.002567978121633	33.07687252617265	20.852058173803588
38	19.542667360896974	26.566315001946204	33.72240560911127	20.16861202804556
39	20.72934622714474	25.89575929754677	33.4990976691049	19.87579680620359
40	18.81915974542642	26.65414693081119	32.73338017702873	21.793313146733663
41	19.57704187118658	26.135748985193686	32.58943691519101	21.69777222842873
42	17.005398820145484	27.92347627400529	33.00584872182428	22.065276184024952
43	17.60998579523913	27.501377507949105	32.82753094969695	22.061105747114816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	139.0
2	170.0
3	439.5
4	709.0
5	709.0
6	1222.0
7	1735.0
8	2006.0
9	2277.0
10	3265.0
11	4253.0
12	4253.0
13	7760.0
14	11267.0
15	18310.0
16	25353.0
17	24541.5
18	23730.0
19	23730.0
20	25556.0
21	27382.0
22	21971.0
23	16560.0
24	17811.0
25	19062.0
26	19062.0
27	20434.5
28	21807.0
29	22178.5
30	22550.0
31	23990.5
32	25431.0
33	25431.0
34	27402.0
35	29373.0
36	32081.0
37	34789.0
38	35577.5
39	36366.0
40	36366.0
41	37976.5
42	39587.0
43	45522.5
44	51458.0
45	66140.5
46	80823.0
47	80823.0
48	82063.5
49	83304.0
50	74762.5
51	66221.0
52	60073.0
53	53925.0
54	53925.0
55	46489.5
56	39054.0
57	31003.5
58	22953.0
59	19521.5
60	16090.0
61	16090.0
62	13937.0
63	11784.0
64	10193.0
65	8602.0
66	7014.5
67	5427.0
68	5427.0
69	4580.0
70	3733.0
71	3023.5
72	2314.0
73	1826.0
74	1338.0
75	1338.0
76	1053.5
77	769.0
78	612.5
79	456.0
80	362.5
81	269.0
82	269.0
83	201.0
84	133.0
85	100.0
86	67.0
87	53.5
88	40.0
89	40.0
90	25.5
91	11.0
92	7.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	791284.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12355639825969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83342615799138	36.677878472258875
2	12.011196036057468	11.320205476314147
3	4.233045853878179	5.984285250949431
4	1.886597496839077	3.5561273417244723
5	0.9986401701423616	2.352973818963562
6	0.6121796333876852	1.7308848887886314
7	0.4222621621834698	1.3928946370152673
8	0.28633519151205195	1.0794506036819733
9	0.23643438108291234	1.0027466002303418
>10	1.3005322287155374	11.353517084192967
>50	0.09101385069775347	2.997054522440745
>100	0.06959872046419027	6.458311117233603
>500	0.012313619774376356	4.090591681494075
>1k	0.005621435114389206	5.7188560648888425
>5k	2.67687386399486E-4	1.2560139809003488
>10k+	5.35374772798972E-4	3.028208458922745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12198	1.5415451342375177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11808	1.492258152572275	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9957	1.2583345549764686	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4752	0.600542915059574	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3953	0.4995677910838586	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3347	0.4229834041886352	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2961	0.37420193002765123	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2887	0.36485004119886155	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2711	0.34260771101147	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2348	0.29673290499997473	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2316	0.2926888449659035	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2122	0.2681717310093468	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2101	0.2655178166119876	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1827	0.23089055257025293	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1819	0.22987953756173513	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1680	0.2123131517887383	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1529	0.1932302435029648	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1500	0.18956531409708777	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1468	0.18552125406301656	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1348	0.17035602893524956	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1344	0.16985052143099066	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1165	0.14722906061540483	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1100	0.1390145636711977	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1058	0.13370673487647924	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	985	0.12448122292375431	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	966	0.12208006227852453	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	961	0.12144817789820089	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	956	0.12081629351787727	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	923	0.11664585660774135	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	915	0.11563484159922355	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	911	0.11512933409496465	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	907	0.11462382659070573	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	901	0.1138655653343174	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	817	0.10324990774488046	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	816	0.10312353086881575	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	814	0.1028707771166863	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.1013542546039096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2637687606472517E-4	0.0	0.0	0.0	0.0
7	1.2637687606472517E-4	0.0	0.0	0.0	0.0
8	1.2637687606472517E-4	0.0	0.0	0.0	0.0
9	1.2637687606472517E-4	0.0	0.0	0.0	0.0
10	1.2637687606472517E-4	0.0	0.0	0.0	0.0
11	1.2637687606472517E-4	0.0	0.0	0.0	0.0
12	1.2637687606472517E-4	0.0	0.0	3.7913062819417554E-4	0.0
13	2.5275375212945034E-4	0.0	0.0	6.318843803236259E-4	0.0
14	2.5275375212945034E-4	0.0	0.0	0.0011373918845825266	0.0
15	2.5275375212945034E-4	0.0	0.0	0.0020220300170356027	0.0
16	2.5275375212945034E-4	0.0	0.0	0.0030330450255534043	0.0
17	2.5275375212945034E-4	0.0	0.0	0.004928698166524282	0.0
18	2.5275375212945034E-4	0.0	0.0	0.006192466927171534	0.0
19	2.5275375212945034E-4	0.0	0.0	0.0073298588117540605	0.0
20	2.5275375212945034E-4	0.0	0.0	0.009225511952724938	0.0
21	2.5275375212945034E-4	0.0	0.0	0.011753049474019442	0.0
22	2.5275375212945034E-4	0.0	0.0	0.017060878268737898	0.0
23	2.5275375212945034E-4	0.0	0.0	0.025528128965074486	0.0
24	2.5275375212945034E-4	0.0	0.0	0.03905045470400008	0.0
25	2.5275375212945034E-4	0.0	0.0	0.04562205225936579	0.0
26	2.5275375212945034E-4	0.0	0.0	0.05901800112222666	0.0
27	2.5275375212945034E-4	0.0	0.0	0.15594906506387088	0.0
28	2.5275375212945034E-4	0.0	0.0	0.25363839026190343	0.0
29	2.5275375212945034E-4	0.0	0.0	0.35941583552807843	0.0
30	2.5275375212945034E-4	0.0	0.0	0.575773047350888	0.0
31	2.5275375212945034E-4	0.0	0.0	0.8944955287861248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2500	0.0	32.412	1
GCATCGG	40	5.937827E-5	27.75	20
AAGACCG	35	8.8669435E-4	26.42857	5
ATTGGAC	215	0.0	24.093025	3
TTGGACC	415	0.0	21.843374	4
GACCCTC	400	0.0	21.275002	7
GTATTGG	245	0.0	21.142857	1
GGACCCT	420	0.0	21.142857	6
TGGACCC	425	0.0	20.894117	5
ACGTTTA	55	5.1411474E-4	20.181818	26
TCACGTT	65	6.898657E-5	19.923077	24
AGCGGGC	75	9.259789E-6	19.733334	26
CTAATAC	105	2.2568202E-8	19.38095	3
GTATCAA	4315	0.0	18.778679	2
AGCGTCG	50	0.0070329914	18.5	33
GTATTAG	90	2.1504911E-6	18.5	1
GTACACT	80	1.6156335E-5	18.5	4
CCGTCAG	60	9.2333485E-4	18.5	14
TCCACGG	100	2.8721115E-7	18.5	2
GCGTCGT	50	0.0070329914	18.5	34
>>END_MODULE