FastQCFastQC Report
Wed 25 May 2016
SRR2088194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274074
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT318810.9737409722565831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT314590.9608518316934803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265010.8094197015705814No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG138500.42302037156154687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113080.3453800983117669No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT80440.24568778836397712No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT74330.2270260232358829No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA71900.21960407736660806No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68160.20818100018509053No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA63000.1924208188330502No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58820.1796538502184129No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG56850.17363688175649053No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT56540.17269004915588346No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45800.13988688099291585No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44960.13732127007514186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.12357692587278114No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT38940.11893439183109483No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37470.11444457272499033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.028.7262541
ATTGGAC9000.022.4055563
GTATTGG9350.022.358291
TATACTG3900.018.0256425
TTGGACC15500.017.783874
GTCGTAC650.001580777217.0769231
GTATCAA132500.016.8664152
GGACCCT15250.016.6196736
AGTGCGC1352.2235326E-816.4444438
TATTGGA13200.016.3977282
TGGACCC15350.016.2703575
TAAACGT1951.8189894E-1216.1282064
GACCCTC16050.015.3302187
CTTGCGA855.367912E-415.23529410
TAGTGCG1101.4534202E-515.1363647
CGTCTGT7600.015.09210634
ACGTTTA2550.014.50980426
ACGTCGC1702.4412657E-814.14705937
GACGTCG1854.9076334E-914.036
TCCAACG800.00630190113.8750012
Produced by FastQC (version 0.11.5)