##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3274074
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.547433564421574	31.0	31.0	33.0	28.0	34.0
2	30.95571236325141	31.0	31.0	34.0	28.0	34.0
3	31.165565897410993	31.0	31.0	34.0	28.0	34.0
4	35.197417651525285	35.0	35.0	37.0	33.0	37.0
5	35.02836466127522	35.0	35.0	37.0	32.0	37.0
6	35.06376489963269	37.0	35.0	37.0	32.0	37.0
7	34.897549963745476	36.0	35.0	37.0	32.0	37.0
8	34.99745240944463	37.0	35.0	37.0	32.0	37.0
9	36.52972657307074	38.0	35.0	39.0	32.0	39.0
10	36.36601310782835	38.0	35.0	39.0	32.0	39.0
11	36.39620393430326	38.0	35.0	39.0	32.0	39.0
12	36.23005344411886	38.0	35.0	39.0	32.0	39.0
13	36.31268688490242	38.0	35.0	39.0	32.0	39.0
14	37.256609349697044	39.0	36.0	41.0	32.0	41.0
15	37.33247110480704	39.0	36.0	41.0	32.0	41.0
16	37.299921138007264	39.0	36.0	41.0	32.0	41.0
17	37.35230602607027	39.0	36.0	41.0	32.0	41.0
18	37.34398153493171	39.0	36.0	41.0	32.0	41.0
19	37.43342056410454	39.0	36.0	41.0	32.0	41.0
20	37.39188454506526	39.0	36.0	41.0	32.0	41.0
21	37.29761972392805	39.0	36.0	41.0	31.0	41.0
22	37.231766905696084	39.0	36.0	40.0	31.0	41.0
23	37.134522005305925	39.0	36.0	40.0	31.0	41.0
24	37.06431589512027	39.0	36.0	40.0	31.0	41.0
25	36.97431029353643	39.0	36.0	40.0	31.0	41.0
26	36.7125150500569	39.0	35.0	40.0	30.0	41.0
27	36.57280715096849	38.0	35.0	40.0	30.0	41.0
28	36.438750620786216	38.0	35.0	40.0	30.0	41.0
29	36.32239283534825	38.0	35.0	40.0	30.0	41.0
30	36.16479529784605	38.0	35.0	40.0	30.0	41.0
31	35.95536325690867	38.0	35.0	40.0	29.0	41.0
32	35.64069382671253	38.0	34.0	40.0	28.0	41.0
33	35.8313297133785	38.0	34.0	40.0	29.0	41.0
34	35.837799939769226	38.0	34.0	40.0	29.0	41.0
35	35.771973693936054	38.0	34.0	40.0	29.0	41.0
36	35.6785185673873	38.0	34.0	40.0	28.0	41.0
37	35.569235454055104	38.0	34.0	40.0	27.0	41.0
38	35.446922702419066	38.0	34.0	40.0	27.0	41.0
39	35.430285937336784	38.0	34.0	40.0	26.0	41.0
40	35.301490131255434	38.0	34.0	40.0	26.0	41.0
41	35.19297517404921	38.0	34.0	40.0	25.0	41.0
42	35.03048159571225	38.0	34.0	40.0	24.0	41.0
43	34.687305784780676	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	9.0
16	45.0
17	103.0
18	252.0
19	544.0
20	1152.0
21	2250.0
22	4099.0
23	7149.0
24	11796.0
25	18847.0
26	28477.0
27	41578.0
28	58161.0
29	78541.0
30	101815.0
31	127594.0
32	156177.0
33	190874.0
34	233708.0
35	283105.0
36	350141.0
37	446832.0
38	561629.0
39	569191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19438778720335	23.472071798010674	13.404858900562418	22.928681514223566
2	17.511760577189154	23.250390797520154	36.03623497819537	23.201613647095332
3	19.6567640193838	25.959889727599318	31.822432846661375	22.560913406355507
4	13.16017903077328	16.245020729525354	36.18940805858389	34.40539218111747
5	11.731164292560278	39.950440949105	35.00815803185878	13.310236726475944
6	31.483527861618278	36.98490015802942	15.830002620588294	15.701569359764012
7	25.15828903073052	34.41168403646344	22.164526519559423	18.265500413246617
8	27.00186373307384	34.33645665919585	20.4397029511245	18.221976656605808
9	25.399089941155882	13.868012757194858	23.071653236915232	37.66124406473403
10	15.967629320534598	27.798577552004016	34.37106186359868	21.8627312638627
11	32.41194304099418	22.25108534504718	24.552071822445065	20.78489979151357
12	20.038673530286733	27.230111475794377	31.99084687762097	20.740368116297923
13	30.202738239880954	21.023990294660415	28.323122812740337	20.450148652718294
14	20.870786671284765	22.32771769972212	28.56566467343133	28.235830955561784
15	23.055709797640493	29.19649952933257	26.191222312018603	21.556568361008335
16	22.08783307891025	28.201378466094535	27.005834321398964	22.704954133596246
17	19.963323981070676	27.55383659624065	28.183083216811838	24.29975620587684
18	20.768864723277485	26.638554901324774	31.467553879356423	21.125026496041325
19	20.46942127758872	25.61970193709733	32.57040005815385	21.34047672716011
20	23.634713204405276	24.690950784863137	31.502983744411395	20.171352266320184
21	23.293120436495936	25.493742658229472	30.44671562096642	20.766421284308173
22	22.00356497745622	25.273985865927283	31.19266699530921	21.52978216130729
23	21.188036678462367	26.532479107069662	31.79213420344195	20.487350011026017
24	20.17578099945206	26.18144855614137	30.828808389792044	22.813962054614525
25	21.253795729723883	26.614731371374013	31.5948570496574	20.5366158492447
26	22.010834208389916	26.057321856500494	30.58544186844891	21.34640206666068
27	21.28177310592247	26.95213975004841	30.26507647658544	21.50101066744368
28	20.837922417147567	26.62847571557637	30.607402276185574	21.92619959109049
29	20.14526855532282	26.67117481156504	31.30271337788944	21.880843255222697
30	19.83779230402245	26.918145405387904	32.401466796413274	20.84259549417637
31	20.938378301773263	26.460519829423525	31.47225749937234	21.12884436943087
32	21.505103427717273	26.610669154087535	30.231570819718794	21.652656598476394
33	22.194122674075174	26.463787929044972	29.879898866061062	21.462190530818788
34	20.991767443252655	28.14792823864091	30.009798190266928	20.850506127839505
35	20.253299100753374	28.692204269054393	30.24653688340581	20.80795974678642
36	20.742109066563554	26.467819603344335	31.47421225054779	21.31585907954432
37	20.809181466271074	26.516444038833576	31.00400296389147	21.67037153100388
38	21.0432018335566	26.26663294720889	31.326903423685597	21.36326179554891
39	21.525353428175418	25.835579770035743	31.324673785626107	21.31439301616274
40	20.438603403588314	26.27918611491371	30.927187351293835	22.355023130204145
41	20.888501603812255	26.020487014038167	30.843469023607895	22.247542358541683
42	19.365689352164917	27.14795084045138	31.01417377860122	22.472186028782488
43	19.800682574676078	26.74285920232713	30.839803865153936	22.616654357842858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	396.0
2	521.0
3	1381.0
4	2241.0
5	2241.0
6	3770.5
7	5300.0
8	5789.0
9	6278.0
10	9328.0
11	12378.0
12	12378.0
13	22194.0
14	32010.0
15	51247.5
16	70485.0
17	69556.5
18	68628.0
19	68628.0
20	73882.5
21	79137.0
22	67753.0
23	56369.0
24	61910.5
25	67452.0
26	67452.0
27	76787.0
28	86122.0
29	92356.0
30	98590.0
31	106658.5
32	114727.0
33	114727.0
34	125965.5
35	137204.0
36	147755.5
37	158307.0
38	165803.0
39	173299.0
40	173299.0
41	180779.0
42	188259.0
43	204635.0
44	221011.0
45	257271.0
46	293531.0
47	293531.0
48	295775.0
49	298019.0
50	273565.5
51	249112.0
52	232335.5
53	215559.0
54	215559.0
55	193167.0
56	170775.0
57	148930.5
58	127086.0
59	114076.5
60	101067.0
61	101067.0
62	88904.5
63	76742.0
64	66471.0
65	56200.0
66	47392.5
67	38585.0
68	38585.0
69	32396.5
70	26208.0
71	22298.0
72	18388.0
73	14438.0
74	10488.0
75	10488.0
76	8114.0
77	5740.0
78	4490.0
79	3240.0
80	2679.5
81	2119.0
82	2119.0
83	1579.0
84	1039.0
85	870.5
86	702.0
87	614.5
88	527.0
89	527.0
90	406.5
91	286.0
92	168.5
93	51.0
94	36.0
95	21.0
96	21.0
97	10.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3274074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.42475249819648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09701999575084	31.307952491730028
2	14.244431028506815	12.371217837810777
3	5.577160362620438	7.265604251686327
4	2.7133664879873263	4.713090727110011
5	1.4510439036906975	3.1505611190892693
6	0.8697777405029284	2.2661929865868142
7	0.5876241891284647	1.7862204482399884
8	0.40158778571314874	1.39510801607138
9	0.29306783461482444	1.145375836499802
>10	1.5192575607948966	12.315192806068808
>50	0.1444117445073286	4.381214016958741
>100	0.09061031882613253	7.52122530081957
>500	0.00539146399389769	1.5997377429701258
>1k	0.004327359258051488	3.6058637065888357
>5k	5.675225256460968E-4	1.632823018009386
>10k+	3.5470157852881054E-4	3.5426196937601193
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31881	0.9737409722565831	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31459	0.9608518316934803	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26501	0.8094197015705814	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13850	0.42302037156154687	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11308	0.3453800983117669	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8044	0.24568778836397712	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7433	0.2270260232358829	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7190	0.21960407736660806	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6816	0.20818100018509053	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6300	0.1924208188330502	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5882	0.1796538502184129	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5685	0.17363688175649053	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5654	0.17269004915588346	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4580	0.13988688099291585	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4496	0.13732127007514186	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4046	0.12357692587278114	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3894	0.11893439183109483	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3747	0.11444457272499033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.054298711635717E-5	0.0
2	0.0	0.0	0.0	9.162896134907153E-5	0.0
3	0.0	0.0	0.0	1.221719484654287E-4	0.0
4	0.0	0.0	0.0	2.1380090981450023E-4	0.0
5	0.0	0.0	0.0	2.1380090981450023E-4	0.0
6	0.0	0.0	0.0	2.1380090981450023E-4	0.0
7	0.0	0.0	0.0	2.443438969308574E-4	0.0
8	3.054298711635717E-5	0.0	0.0	2.7488688404721457E-4	0.0
9	3.054298711635717E-5	0.0	0.0	5.803167552107864E-4	0.0
10	3.054298711635717E-5	0.0	0.0	8.857466263743581E-4	0.0
11	3.054298711635717E-5	0.0	0.0	0.0013438914331197157	0.0
12	3.054298711635717E-5	0.0	0.0	0.0016798642913996445	0.0
13	3.054298711635717E-5	0.0	0.0	0.0017714932527487161	0.0
14	3.054298711635717E-5	0.0	0.0	0.001954751175446859	0.0
15	3.054298711635717E-5	0.0	0.0	0.0025350679306576454	0.0
16	3.054298711635717E-5	0.0	0.0	0.003329185595682932	0.0
17	3.054298711635717E-5	0.0	0.0	0.004459276118988147	0.0
18	3.054298711635717E-5	0.0	0.0	0.005039592874198934	0.0
19	3.054298711635717E-5	0.0	0.0	0.005833710539224221	0.0
20	3.054298711635717E-5	0.0	0.0	0.0068416291140640075	0.0
21	3.054298711635717E-5	0.0	0.0	0.008735294315278152	0.0
22	3.054298711635717E-5	0.0	0.0	0.013774887189477086	0.0
23	3.054298711635717E-5	0.0	0.0	0.02278506838880245	0.0
24	3.054298711635717E-5	0.0	0.0	0.03509389219669439	0.0
25	3.054298711635717E-5	0.0	0.0	0.039644797277031615	0.0
26	3.054298711635717E-5	0.0	0.0	0.04841063457942612	0.0
27	3.054298711635717E-5	0.0	0.0	0.11099321518084197	0.0
28	3.054298711635717E-5	0.0	0.0	0.17449208539574854	0.0
29	3.054298711635717E-5	0.0	0.0	0.2537511369626954	0.0
30	3.054298711635717E-5	0.0	0.0	0.39330204509733135	0.0
31	3.054298711635717E-5	0.0	0.0	0.613547525193383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7580	0.0	28.726254	1
ATTGGAC	900	0.0	22.405556	3
GTATTGG	935	0.0	22.35829	1
TATACTG	390	0.0	18.025642	5
TTGGACC	1550	0.0	17.78387	4
GTCGTAC	65	0.0015807772	17.076923	1
GTATCAA	13250	0.0	16.866415	2
GGACCCT	1525	0.0	16.619673	6
AGTGCGC	135	2.2235326E-8	16.444443	8
TATTGGA	1320	0.0	16.397728	2
TGGACCC	1535	0.0	16.270357	5
TAAACGT	195	1.8189894E-12	16.128206	4
GACCCTC	1605	0.0	15.330218	7
CTTGCGA	85	5.367912E-4	15.235294	10
TAGTGCG	110	1.4534202E-5	15.136364	7
CGTCTGT	760	0.0	15.092106	34
ACGTTTA	255	0.0	14.509804	26
ACGTCGC	170	2.4412657E-8	14.147059	37
GACGTCG	185	4.9076334E-9	14.0	36
TCCAACG	80	0.006301901	13.875001	2
>>END_MODULE