FastQCFastQC Report
Wed 25 May 2016
SRR2088193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402938
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT374721.5594243380395165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369541.5378673939985135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT328021.3650789158937935No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG183170.7622751814653561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126150.5249823341259741No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT105640.43962848812578603No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA100470.41811315980686975No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA99100.4124118058809674No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97880.4073346877863682No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA85670.35652189111828936No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT82630.3438707116038782No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT79350.33022075475938206No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG73100.3042109284550829No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA67020.2789085694262607No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60860.25327328462074344No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT58550.24366005281867448No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA52650.21910677678741605No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48810.20312633950605466No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.18993415560451415No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC45010.18731236511304078No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41620.17320463532558894No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36630.15243839000423648No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35390.14727804046546353No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34460.1434077783113838No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34400.14315808397886254No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32810.13654118416704886No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.12488878198272282No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA28660.11927065950099419No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC27550.11465131434935066No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC27470.11431838857265564No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27200.11319476407630992No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC26860.11177982952535605No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC26340.10961581197683834No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26070.10849218748049264No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA26040.108367340314232No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA24910.10366476371841471No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC24820.1032902222196328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC24200.10071004745024632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81050.029.9241181
CTGTGCG1002.0008883E-1124.0500019
GTATTGG7200.022.611111
ATTGGAC7500.022.4466693
AGCCGTC1800.018.512
TTGGACC13250.017.7320754
TATTGGA9050.017.580112
GGACCCT13400.017.3955236
GTATCAA142950.017.289962
TCACGTT1502.5102054E-1017.26666624
ACGTTTA1401.87174E-917.17857226
ACGATCT3400.016.86764532
CGATCTG3400.016.86764533
CAATGCG1107.816325E-716.81818219
CGGTGTG1554.0199666E-1016.70967730
CGGGATA1452.9849616E-916.58620624
TTTTTAC20400.016.2328431
ATCACGT1606.311893E-1016.187523
CGACGAG1151.2434975E-616.08695624
GTACTAG2300.016.0869561
Produced by FastQC (version 0.11.5)