##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2402938
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.525658589609886	31.0	31.0	33.0	28.0	34.0
2	30.912172515478968	31.0	31.0	34.0	27.0	34.0
3	31.09574945337749	31.0	31.0	34.0	28.0	34.0
4	35.140160503516945	35.0	35.0	37.0	32.0	37.0
5	35.00203584112449	35.0	35.0	37.0	32.0	37.0
6	35.04518926414248	36.0	35.0	37.0	32.0	37.0
7	34.85304864295292	36.0	35.0	37.0	32.0	37.0
8	34.96714313894075	36.0	35.0	37.0	32.0	37.0
9	36.42866732308532	38.0	35.0	39.0	32.0	39.0
10	36.309543150926075	38.0	35.0	39.0	32.0	39.0
11	36.34547291690422	38.0	35.0	39.0	32.0	39.0
12	36.181849885431916	38.0	35.0	39.0	31.0	39.0
13	36.253408535717526	38.0	35.0	39.0	32.0	39.0
14	37.10923253117642	39.0	36.0	40.0	31.0	41.0
15	37.18465853051556	39.0	36.0	41.0	32.0	41.0
16	37.147996327828686	39.0	36.0	40.0	31.0	41.0
17	37.27971924369251	39.0	36.0	40.0	32.0	41.0
18	37.30296745067913	39.0	36.0	40.0	32.0	41.0
19	37.418983344555706	39.0	36.0	41.0	32.0	41.0
20	37.384861781702234	39.0	36.0	41.0	32.0	41.0
21	37.282300250776345	39.0	36.0	41.0	31.0	41.0
22	37.22579067791179	39.0	36.0	40.0	31.0	41.0
23	37.09812030106478	39.0	36.0	40.0	31.0	41.0
24	37.032382441827465	39.0	36.0	40.0	31.0	41.0
25	36.93846949026567	39.0	35.0	40.0	31.0	41.0
26	36.65541974033454	38.0	35.0	40.0	30.0	41.0
27	36.50473087528684	38.0	35.0	40.0	30.0	41.0
28	36.34206126000754	38.0	35.0	40.0	30.0	41.0
29	36.203830061366546	38.0	35.0	40.0	30.0	41.0
30	36.01323463193807	38.0	35.0	40.0	30.0	41.0
31	35.70825006720939	38.0	34.0	40.0	29.0	41.0
32	35.38702413462187	38.0	34.0	40.0	27.0	41.0
33	35.48150680541903	38.0	34.0	40.0	28.0	41.0
34	35.43730591467612	38.0	34.0	40.0	27.0	41.0
35	35.32626767731835	38.0	34.0	40.0	27.0	41.0
36	35.20769532963397	38.0	34.0	40.0	26.0	41.0
37	35.05497062346178	38.0	34.0	40.0	25.0	41.0
38	34.89194727454475	38.0	33.0	40.0	25.0	41.0
39	34.78711435750735	38.0	33.0	40.0	24.0	41.0
40	34.57909151214056	38.0	33.0	40.0	23.0	41.0
41	34.408324725814815	38.0	33.0	40.0	22.0	41.0
42	34.15766615701279	38.0	33.0	40.0	21.0	41.0
43	33.794057524580325	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	28.0
17	71.0
18	189.0
19	446.0
20	984.0
21	1870.0
22	3405.0
23	5989.0
24	9867.0
25	15377.0
26	23365.0
27	34303.0
28	47731.0
29	64305.0
30	81571.0
31	99886.0
32	121094.0
33	146698.0
34	176943.0
35	212073.0
36	260918.0
37	334037.0
38	384941.0
39	376833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9866330300657	24.161547239254613	14.49155159225914	21.360268138420548
2	17.209016628810232	23.396733498741956	36.85226168964826	22.541988182799557
3	19.813120438396663	26.26905063717832	32.96631040834178	20.95151851608323
4	12.616721696523173	15.611763599393742	35.197703810918135	36.573810893164946
5	10.573472973501605	41.32740836426075	34.56293920192697	13.536179460310668
6	31.11586732574873	35.94474763809969	15.376634769602878	17.562750266548697
7	23.686170845856196	36.278963502179415	21.272042807596367	18.762822844368017
8	28.10322197243541	33.27314312728834	20.281089233263614	18.342545667012633
9	24.947293687976966	13.482869720317378	24.71104123368976	36.8587953580159
10	16.231713011321975	28.2253641167604	33.60989755041537	21.93302532150226
11	30.560547130221423	21.641174262506983	25.964590014390716	21.833688592880883
12	20.20097896824637	27.929143406945993	32.096500201003934	19.77337742380369
13	31.346834583330907	20.984353320809777	29.810506971049605	17.85830512480971
14	20.59270776025016	22.701376398392302	29.657194650881546	27.048721190475995
15	22.51248263583996	29.018185238237525	28.26040455475755	20.208927571164967
16	21.033376641428116	28.545930023995624	28.08965524703509	22.33103808754117
17	18.447001129450697	27.173568356736627	28.756630424921493	25.622800088891186
18	19.744579344119572	26.305797319781032	34.201340192714085	19.748283143385308
19	18.770022364289048	24.562181795784994	35.865802613300886	20.801993226625072
20	24.066829855784878	23.2681409174935	34.25943574074737	18.405593485974254
21	23.26963908348863	24.346862049707482	32.788028654921604	19.595470211882287
22	21.081026643217594	23.890753735635293	33.8013714877371	21.226848133410016
23	19.9604817103063	26.354321251734337	35.06923607683594	18.615960961123424
24	18.3153706004899	25.363950297510797	33.35125583764542	22.969423264353885
25	19.85448646615102	26.43247557781349	34.684207416088135	19.028830539947347
26	21.528270808485278	25.20847395979422	32.881996955393774	20.38125827632673
27	19.771338253421437	26.998324551028784	32.489893621891206	20.740443573658577
28	19.487602260233096	26.324149853221346	32.606292796568205	21.581955089977352
29	18.42706719857108	26.309917276267637	33.70690379859988	21.5561117265614
30	17.68701481270012	26.367014046970834	36.240302496360705	19.70566864396834
31	19.508160426944016	25.840658394015993	34.63193806914702	20.019243109892972
32	20.94506807915976	25.99963045238787	32.215812476227015	20.83948899222535
33	22.044139299474228	26.012240016180193	31.448293713778714	20.495326970566865
34	19.672167987688404	29.42805848507119	31.686751801336534	19.21302172590387
35	18.108207535941418	30.455592279118314	31.792705429769725	19.643494755170547
36	19.35131077039857	26.13521447494692	34.34233425914443	20.17114049551008
37	19.393384265428402	26.667479560438096	33.22337072367244	20.71576545046106
38	20.010961581197684	26.079366175906326	33.8013714877371	20.108300755158893
39	21.059885856397457	25.52745846959014	33.4860907772069	19.926564896805495
40	19.038568618915676	26.467307937200214	32.41086536564822	22.083258078235893
41	20.249835825976366	25.91215420456125	32.15047579255062	21.687534176911765
42	17.325873576430187	27.9329304376559	32.813289398228335	21.927906587685577
43	18.168508717245306	27.81045536755422	32.12750391395866	21.893532001241812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	293.0
2	410.0
3	1147.0
4	1884.0
5	1884.0
6	3266.0
7	4648.0
8	5374.5
9	6101.0
10	9358.0
11	12615.0
12	12615.0
13	22939.5
14	33264.0
15	56017.0
16	78770.0
17	76468.5
18	74167.0
19	74167.0
20	77201.0
21	80235.0
22	63883.5
23	47532.0
24	50996.0
25	54460.0
26	54460.0
27	57385.0
28	60310.0
29	62220.5
30	64131.0
31	68415.0
32	72699.0
33	72699.0
34	78588.5
35	84478.0
36	91660.5
37	98843.0
38	102038.0
39	105233.0
40	105233.0
41	111898.5
42	118564.0
43	138037.5
44	157511.0
45	208035.5
46	258560.0
47	258560.0
48	261899.0
49	265238.0
50	236252.0
51	207266.0
52	189731.0
53	172196.0
54	172196.0
55	145566.0
56	118936.0
57	93433.5
58	67931.0
59	57761.5
60	47592.0
61	47592.0
62	41721.5
63	35851.0
64	30530.0
65	25209.0
66	21246.0
67	17283.0
68	17283.0
69	14619.5
70	11956.0
71	9906.0
72	7856.0
73	6317.0
74	4778.0
75	4778.0
76	3827.0
77	2876.0
78	2188.5
79	1501.0
80	1197.0
81	893.0
82	893.0
83	676.0
84	459.0
85	364.0
86	269.0
87	206.0
88	143.0
89	143.0
90	115.0
91	87.0
92	51.5
93	16.0
94	11.5
95	7.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2402938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.382947286341704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38795791079376	27.784631726559184
2	14.036226439899336	10.775034790836235
3	5.43225055734919	6.255173603668028
4	2.664595840338085	4.091001667164083
5	1.4309573766769894	2.7462180778997345
6	0.8782987955859214	2.022701782357909
7	0.5594639786392458	1.5031713480502022
8	0.39478036440231307	1.2122267133229399
9	0.2961556004233567	1.0230592319644118
>10	1.6437370375018097	11.94606493968749
>50	0.15809224104734731	4.220462876949849
>100	0.09690232569292692	7.087849681634573
>500	0.009852860896180066	2.5461722707844965
>1k	0.00886757480656206	6.934510306350859
>5k	0.001094762321797785	3.1801310959045694
>10k+	7.663336252584495E-4	6.671589886865455
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37472	1.5594243380395165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36954	1.5378673939985135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32802	1.3650789158937935	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18317	0.7622751814653561	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12615	0.5249823341259741	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10564	0.43962848812578603	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10047	0.41811315980686975	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9910	0.4124118058809674	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9788	0.4073346877863682	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8567	0.35652189111828936	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8263	0.3438707116038782	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7935	0.33022075475938206	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7310	0.3042109284550829	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6702	0.2789085694262607	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6086	0.25327328462074344	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5855	0.24366005281867448	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5265	0.21910677678741605	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4881	0.20312633950605466	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4564	0.18993415560451415	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4501	0.18731236511304078	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4162	0.17320463532558894	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3663	0.15243839000423648	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3539	0.14727804046546353	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3446	0.1434077783113838	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3440	0.14315808397886254	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3281	0.13654118416704886	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3001	0.12488878198272282	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2866	0.11927065950099419	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2755	0.11465131434935066	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2747	0.11431838857265564	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2720	0.11319476407630992	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2686	0.11177982952535605	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2634	0.10961581197683834	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2607	0.10849218748049264	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2604	0.108367340314232	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2491	0.10366476371841471	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2482	0.1032902222196328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2420	0.10071004745024632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.4969433252127187E-4	0.0	0.0	0.0	0.0
7	2.4969433252127187E-4	0.0	0.0	4.161572208687865E-5	0.0
8	2.9131005460815055E-4	0.0	0.0	8.32314441737573E-5	0.0
9	2.9131005460815055E-4	0.0	0.0	1.2484716626063593E-4	0.0
10	2.9131005460815055E-4	0.0	0.0	1.664628883475146E-4	0.0
11	3.329257766950292E-4	0.0	0.0	4.577729429556651E-4	0.0
12	3.329257766950292E-4	0.0	0.0	8.32314441737573E-4	0.0
13	3.329257766950292E-4	0.0	0.0	0.001040393052171966	0.0
14	4.577729429556651E-4	0.0	0.0	0.0012484716626063594	0.0
15	4.577729429556651E-4	0.0	0.0	0.0022472489926914467	0.0
16	4.577729429556651E-4	0.0	0.0	0.003329257766950292	0.0
17	4.577729429556651E-4	0.0	0.0	0.0052435809829467096	0.0
18	4.577729429556651E-4	0.0	0.0	0.005784585370076132	0.0
19	4.577729429556651E-4	0.0	0.0	0.006783362700161219	0.0
20	4.993886650425437E-4	0.0	0.0	0.008447991583636365	0.0
21	4.993886650425437E-4	0.0	0.0	0.011694017906412899	0.0
22	4.993886650425437E-4	0.0	4.161572208687865E-5	0.019309695048311693	0.0
23	4.993886650425437E-4	0.0	4.161572208687865E-5	0.030296245679247654	0.0
24	4.993886650425437E-4	0.0	4.161572208687865E-5	0.0473586917348679	0.0
25	4.993886650425437E-4	0.0	4.161572208687865E-5	0.05489113743259293	0.0
26	4.993886650425437E-4	0.0	4.161572208687865E-5	0.06479567928927005	0.0
27	5.410043871294224E-4	0.0	4.161572208687865E-5	0.1338361622314017	0.0
28	5.826201092163011E-4	0.0	4.161572208687865E-5	0.2050406627220511	0.0
29	5.826201092163011E-4	0.0	4.161572208687865E-5	0.2943063865984058	0.0
30	5.826201092163011E-4	0.0	4.161572208687865E-5	0.4769161751156293	0.0
31	7.906987196506942E-4	0.0	4.161572208687865E-5	0.7312298527885447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8105	0.0	29.924118	1
CTGTGCG	100	2.0008883E-11	24.050001	9
GTATTGG	720	0.0	22.61111	1
ATTGGAC	750	0.0	22.446669	3
AGCCGTC	180	0.0	18.5	12
TTGGACC	1325	0.0	17.732075	4
TATTGGA	905	0.0	17.58011	2
GGACCCT	1340	0.0	17.395523	6
GTATCAA	14295	0.0	17.28996	2
TCACGTT	150	2.5102054E-10	17.266666	24
ACGTTTA	140	1.87174E-9	17.178572	26
ACGATCT	340	0.0	16.867645	32
CGATCTG	340	0.0	16.867645	33
CAATGCG	110	7.816325E-7	16.818182	19
CGGTGTG	155	4.0199666E-10	16.709677	30
CGGGATA	145	2.9849616E-9	16.586206	24
TTTTTAC	2040	0.0	16.232843	1
ATCACGT	160	6.311893E-10	16.1875	23
CGACGAG	115	1.2434975E-6	16.086956	24
GTACTAG	230	0.0	16.086956	1
>>END_MODULE