FastQCFastQC Report
Wed 25 May 2016
SRR2088192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545187
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185151.1982368477084002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183051.1846462596436549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144210.9332850975318845No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG74600.4827894617285804No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65050.42098464457699947No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA48010.3107067299944926No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT46650.3019052062954193No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT46100.2983457665641764No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44530.2881851840586285No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37300.2413947308642902No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG35520.22987508955226776No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32780.21214260798207593No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32470.21013637831537543No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25950.1679408382286416No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25140.16269875426081115No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT23170.14994948831435936No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23170.14994948831435936No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.14639004858311647No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20150.1304049283355348No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18510.11979132622782873No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.11429037391590792No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17230.1115075392169362No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16540.10704206028137694No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16140.10445337684047303No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16060.10393564015229226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42550.029.739131
ACCGTCC450.003826395820.5555558
TCCGACG851.2458768E-619.58823623
GTATCAA68600.018.7427122
CGACGAG801.6172573E-518.524
ATTGGAC4450.017.8764063
TATACCG1107.811868E-716.8181825
GTATTGG5000.016.651
TGGACCC7700.016.3376625
TGCGACG803.3835077E-416.187522
GAACCGA700.00259303515.8571436
CAATGCG957.062189E-515.57894819
CGATCTG1802.0190782E-1015.41666633
TATACTG2055.456968E-1215.3414645
AGACGAG855.365635E-415.2352945
TTGGACC8200.015.1158544
CGAGTTG750.00410525714.832
GGACTGA1508.1059625E-814.86
TTATGCG750.00410525714.84
CGACCCG1001.0937512E-414.7999995
Produced by FastQC (version 0.11.5)