##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1545187
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.352976694730152	31.0	30.0	31.0	27.0	34.0
2	30.706829658805052	31.0	31.0	34.0	27.0	34.0
3	30.870027381799098	31.0	31.0	34.0	27.0	34.0
4	34.89652708701277	35.0	35.0	37.0	32.0	37.0
5	34.70989012980306	35.0	35.0	37.0	32.0	37.0
6	34.776671690869776	35.0	35.0	37.0	32.0	37.0
7	34.603980618527075	35.0	35.0	37.0	30.0	37.0
8	34.714143983867324	35.0	35.0	37.0	31.0	37.0
9	36.16884817177468	38.0	35.0	39.0	32.0	39.0
10	35.96650631929987	37.0	35.0	39.0	30.0	39.0
11	36.0291725208664	37.0	35.0	39.0	30.0	39.0
12	35.840550043457526	37.0	35.0	39.0	30.0	39.0
13	35.933028170700375	37.0	35.0	39.0	30.0	39.0
14	36.77075201901129	38.0	36.0	40.0	31.0	41.0
15	36.85004921734392	38.0	36.0	40.0	31.0	41.0
16	36.79690548781474	38.0	36.0	40.0	31.0	41.0
17	36.87658710563834	38.0	36.0	40.0	31.0	41.0
18	36.88338628269588	38.0	36.0	40.0	31.0	41.0
19	36.97620093878605	39.0	36.0	40.0	31.0	41.0
20	36.94998469440915	39.0	36.0	40.0	31.0	41.0
21	36.855905466458104	39.0	36.0	40.0	31.0	41.0
22	36.78495159485551	39.0	35.0	40.0	30.0	41.0
23	36.66030454566341	38.0	35.0	40.0	30.0	41.0
24	36.5851382389316	38.0	35.0	40.0	30.0	41.0
25	36.48814997796383	38.0	35.0	40.0	30.0	41.0
26	36.20416622712979	38.0	35.0	40.0	30.0	41.0
27	36.040487009015735	38.0	34.0	40.0	29.0	41.0
28	35.88118201874595	38.0	34.0	40.0	29.0	41.0
29	35.73776572026557	38.0	34.0	40.0	28.0	41.0
30	35.56997632001823	38.0	34.0	40.0	27.0	41.0
31	35.31465770809617	38.0	34.0	40.0	27.0	41.0
32	35.02514905962838	37.0	33.0	40.0	26.0	41.0
33	35.20548192548863	38.0	33.0	40.0	27.0	41.0
34	35.18991487761676	38.0	33.0	40.0	27.0	41.0
35	35.10108614685472	38.0	33.0	40.0	26.0	41.0
36	34.995629008010035	38.0	33.0	40.0	25.0	41.0
37	34.87855579939515	38.0	33.0	40.0	25.0	41.0
38	34.74245447314791	38.0	33.0	40.0	25.0	41.0
39	34.70269100115391	38.0	33.0	40.0	24.0	41.0
40	34.54900992565948	38.0	33.0	40.0	24.0	41.0
41	34.412787578461376	38.0	33.0	40.0	23.0	41.0
42	34.21859037126251	38.0	33.0	40.0	22.0	41.0
43	33.864298625344375	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	23.0
17	79.0
18	208.0
19	378.0
20	770.0
21	1599.0
22	2834.0
23	4703.0
24	7801.0
25	11909.0
26	17506.0
27	24815.0
28	34152.0
29	45261.0
30	56930.0
31	69754.0
32	83915.0
33	99973.0
34	119040.0
35	141114.0
36	172045.0
37	213087.0
38	256999.0
39	180283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.02252607613189	24.794021694461577	14.403952401877573	21.779499827528966
2	18.451358961730847	24.297965230098363	35.09588159879678	22.15479420937401
3	20.815215245792256	26.207378136109092	32.00661149750807	20.970795120590584
4	14.029758210494911	16.851229009822113	35.47162900024398	33.647383779439
5	11.487088617753063	40.72465015561224	34.68110979447795	13.107151432156755
6	31.692668913212447	37.06819951242147	15.12528904268545	16.113842531680632
7	24.272337264033414	35.23366427493889	22.03293193639346	18.461066524634234
8	26.980164860304935	34.55089901740048	20.45156993943128	18.017366182863302
9	24.96125064474397	13.791016880157548	23.63183226366776	37.61590021143072
10	16.129568783584123	28.126563322109234	34.31759392228902	21.426273972017626
11	31.985125424948567	22.099396383738668	24.755903330794265	21.1595748605185
12	19.59212703705118	27.608244180154244	32.13779303087587	20.6618357519187
13	30.47042202658966	21.05343883944144	28.983999994822636	19.492139139146264
14	20.56042407812129	22.64101367666179	28.83521541405668	27.96334683116024
15	22.839436262407077	29.519533881659633	26.66318057296625	20.977849282967046
16	21.49830408876078	28.51357149652437	27.495507016302884	22.49261739841197
17	19.390727465348853	27.56902562602455	28.681512334753013	24.358734573873583
18	20.39151248360231	26.374995388907617	32.500920600548675	20.7325715269414
19	19.73049216696749	25.375439995288595	33.7556554643548	21.138412373389112
20	23.376070339706455	24.46804173216575	32.60285001103426	19.55303791709353
21	23.22644443682221	25.020466778454647	31.389404648110553	20.36368413661259
22	21.668251156656122	24.86158633226917	32.0413645727022	21.428797938372508
23	20.76939554888826	26.6610449091275	32.85634683698478	19.71321270499946
24	19.760391460709933	25.85913549622149	31.69273363029847	22.687739412770107
25	20.67775615508026	26.540282826609335	32.61339889605595	20.168562122254457
26	21.770504152571824	25.901848772996406	31.45988155478916	20.867765519642607
27	20.837995660072213	26.973499000444605	31.095071340879777	21.093433998603405
28	20.572849758637627	26.538535465286728	31.488357072639104	21.400257703436544
29	19.822260994947538	26.37823124320875	32.07165216896078	21.727855592882932
30	19.26931821197046	26.636193548094827	33.520926593350836	20.573561646583876
31	20.424324046215766	26.08493340935434	32.75357610438089	20.737166440049005
32	21.23484083156278	26.38664446439169	30.96240131453345	21.416113389512077
33	22.000249807952045	26.343219299670523	30.696737676410685	20.95979321596674
34	20.76596554332906	28.441994399383375	30.71524676301315	20.076793294274413
35	19.71295383665537	29.066125976985308	30.88059891780089	20.340321268558434
36	20.435520102097676	26.229834964958933	32.5336674460761	20.800977486867286
37	20.44263898156016	26.527662994834927	31.838670659279426	21.191027364325482
38	20.790558036017647	26.009602721224034	32.360225655535544	20.83961358722278
39	21.501798811405997	25.76018307169294	32.12057828599386	20.6174398309072
40	20.178204968071825	26.36399348428378	31.448103045133045	22.009698502511345
41	20.697883168833286	26.078979437440257	31.36908348309946	21.854053910626998
42	18.945473913513382	27.443280327882647	31.48473291582184	22.126512842782137
43	19.390662748262834	27.21353467250242	31.139790847321393	22.256011731913354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	174.0
2	230.0
3	532.0
4	834.0
5	834.0
6	1668.5
7	2503.0
8	2820.5
9	3138.0
10	4695.0
11	6252.0
12	6252.0
13	11799.5
14	17347.0
15	28514.5
16	39682.0
17	38369.5
18	37057.0
19	37057.0
20	40261.5
21	43466.0
22	36303.5
23	29141.0
24	32044.5
25	34948.0
26	34948.0
27	38315.0
28	41682.0
29	44129.5
30	46577.0
31	50017.0
32	53457.0
33	53457.0
34	57639.0
35	61821.0
36	67367.5
37	72914.0
38	75688.0
39	78462.0
40	78462.0
41	81978.5
42	85495.0
43	94100.5
44	102706.0
45	122747.0
46	142788.0
47	142788.0
48	144904.0
49	147020.0
50	133979.0
51	120938.0
52	112942.5
53	104947.0
54	104947.0
55	90816.0
56	76685.0
57	65213.5
58	53742.0
59	47264.0
60	40786.0
61	40786.0
62	35922.5
63	31059.0
64	27182.5
65	23306.0
66	19705.0
67	16104.0
68	16104.0
69	13625.5
70	11147.0
71	9498.5
72	7850.0
73	6273.0
74	4696.0
75	4696.0
76	3657.0
77	2618.0
78	2049.0
79	1480.0
80	1202.0
81	924.0
82	924.0
83	726.0
84	528.0
85	427.0
86	326.0
87	282.0
88	238.0
89	238.0
90	192.0
91	146.0
92	82.5
93	19.0
94	13.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1545187.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.503833467718174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87066231193951	40.62141457202769
2	12.248893094753173	12.617299002321012
3	3.8295133957673992	5.917038605940002
4	1.683424950968087	3.4681135332024797
5	0.8452659652028047	2.176721875386766
6	0.5156896943544511	1.593599768343008
7	0.32395399463912017	1.1679410813766717
8	0.23639871209002772	0.9740351919574274
9	0.17563939591546376	0.8141491977840599
>10	1.1059266084079	10.837589126365527
>50	0.10038121467127586	3.584672679720059
>100	0.05347893808685019	5.00745275588327
>500	0.006209581611708391	2.403394053696756
>1k	0.003928510815570615	4.560659356979628
>5k	2.5345231068197514E-4	0.911479183115857
>10k+	3.8017846602296274E-4	3.3444400158997225
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18515	1.1982368477084002	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18305	1.1846462596436549	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14421	0.9332850975318845	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7460	0.4827894617285804	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6505	0.42098464457699947	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4801	0.3107067299944926	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4665	0.3019052062954193	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4610	0.2983457665641764	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4453	0.2881851840586285	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3730	0.2413947308642902	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3552	0.22987508955226776	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3278	0.21214260798207593	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3247	0.21013637831537543	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2595	0.1679408382286416	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2514	0.16269875426081115	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2317	0.14994948831435936	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2317	0.14994948831435936	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2262	0.14639004858311647	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2015	0.1304049283355348	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1851	0.11979132622782873	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.11429037391590792	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1723	0.1115075392169362	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1654	0.10704206028137694	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1614	0.10445337684047303	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1606	0.10393564015229226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.471708602259791E-5	0.0
8	0.0	0.0	0.0	6.471708602259791E-5	0.0
9	0.0	0.0	0.0	1.2943417204519582E-4	0.0
10	0.0	0.0	0.0	3.2358543011298954E-4	0.0
11	0.0	0.0	0.0	4.530196021581854E-4	0.0
12	0.0	0.0	0.0	0.0011649075484067625	0.0
13	0.0	0.0	0.0	0.0012943417204519581	0.0
14	0.0	0.0	0.0	0.0016179271505649478	0.0
15	0.0	0.0	0.0	0.0023945321828361225	0.0
16	0.0	0.0	0.0	0.004012459333401071	0.0
17	0.0	0.0	0.0	0.00556566939794342	0.0
18	0.0	0.0	0.0	0.006018689000101606	0.0
19	0.0	0.0	0.0	0.0071835965485083685	0.0
20	0.0	0.0	0.0	0.008736806613050718	0.0
21	0.0	0.0	0.0	0.012037378000203212	0.0
22	0.0	0.0	0.0	0.018962106204621187	6.471708602259791E-5
23	0.0	0.0	0.0	0.02976985957039504	6.471708602259791E-5
24	0.0	0.0	0.0	0.046725736108315694	6.471708602259791E-5
25	0.0	0.0	0.0	0.052873859280462496	6.471708602259791E-5
26	0.0	0.0	0.0	0.06012217291499346	6.471708602259791E-5
27	0.0	0.0	0.0	0.1148728276901113	6.471708602259791E-5
28	0.0	0.0	0.0	0.17137084378783926	6.471708602259791E-5
29	0.0	0.0	0.0	0.23906491576747668	6.471708602259791E-5
30	0.0	0.0	0.0	0.3717349421138024	6.471708602259791E-5
31	0.0	0.0	0.0	0.5736522505043079	6.471708602259791E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4255	0.0	29.73913	1
ACCGTCC	45	0.0038263958	20.555555	8
TCCGACG	85	1.2458768E-6	19.588236	23
GTATCAA	6860	0.0	18.742712	2
CGACGAG	80	1.6172573E-5	18.5	24
ATTGGAC	445	0.0	17.876406	3
TATACCG	110	7.811868E-7	16.818182	5
GTATTGG	500	0.0	16.65	1
TGGACCC	770	0.0	16.337662	5
TGCGACG	80	3.3835077E-4	16.1875	22
GAACCGA	70	0.002593035	15.857143	6
CAATGCG	95	7.062189E-5	15.578948	19
CGATCTG	180	2.0190782E-10	15.416666	33
TATACTG	205	5.456968E-12	15.341464	5
AGACGAG	85	5.365635E-4	15.235294	5
TTGGACC	820	0.0	15.115854	4
CGAGTTG	75	0.004105257	14.8	32
GGACTGA	150	8.1059625E-8	14.8	6
TTATGCG	75	0.004105257	14.8	4
CGACCCG	100	1.0937512E-4	14.799999	5
>>END_MODULE