FastQCFastQC Report
Wed 25 May 2016
SRR2088191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732554
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT400751.4665766897927726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT392261.4355068554912365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT348111.273936397963224No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG214140.783662463761009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133410.48822456939551795No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT119130.43596576682473615No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA114130.4176678667649386No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT111490.4080065755333655No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105610.3864882450630436No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA93700.34290264712060586No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT89160.32628815386630966No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT87260.3193349518435866No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG83990.30736812520447904No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72790.26638082907053257No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA71090.2601595430502014No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT61550.2252471497361077No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59220.21672032830824203No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48860.17880707938434154No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48150.1762087775758503No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC47560.17404962536879418No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43320.15853300611808585No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42740.15641044971114934No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39960.14623681727790191No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38460.14074744725996266No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT37400.1368682924472856No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36240.13262317963341255No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA33020.12083933199490293No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC31600.11564272837792043No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG30610.11201974416608053No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA30590.11194655256584134No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC30540.11176357356524336No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.1107388911618947No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC28690.10499335054311827No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC28640.10481037154252029No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA27840.10188270753295268No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC27780.10166313273223511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87050.031.4106831
GTATTGG7050.024.6666661
ATTGGAC7500.022.6933353
TATACCG1600.021.9687525
TATTGGA8650.019.462432
GGACCCT14300.018.8881136
TTGGACC15300.018.54
CAATGCG1002.8785325E-718.519
GTATCAA153200.018.222262
TGGACCC15800.017.0949365
TCCGATA650.001580686817.0769238
CGTCTGT8900.017.04494334
GACCCTC16750.016.346277
TTTTTAC21600.016.1018521
ACCGACC2200.015.9772738
CGAACGA700.00259371915.85714316
CGCCGGA1403.4786353E-815.85714314
TGCGACG1059.352443E-615.85714222
CGGGATA2250.015.62222224
CTGTGCG1353.977675E-715.0740759
Produced by FastQC (version 0.11.5)