##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2732554
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.622492730244307	31.0	31.0	33.0	28.0	34.0
2	30.982611505573175	31.0	31.0	34.0	28.0	34.0
3	31.153011431796042	31.0	31.0	34.0	28.0	34.0
4	35.1810899253958	35.0	35.0	37.0	33.0	37.0
5	35.03591219057336	35.0	35.0	37.0	32.0	37.0
6	35.083850858940025	37.0	35.0	37.0	32.0	37.0
7	34.90744483000153	36.0	35.0	37.0	32.0	37.0
8	35.01493913752482	37.0	35.0	37.0	32.0	37.0
9	36.48902052804812	38.0	35.0	39.0	32.0	39.0
10	36.367454769420846	38.0	35.0	39.0	32.0	39.0
11	36.40147422521202	38.0	35.0	39.0	32.0	39.0
12	36.24727306395409	38.0	35.0	39.0	32.0	39.0
13	36.31880907019587	38.0	35.0	39.0	32.0	39.0
14	37.216406702301214	39.0	36.0	41.0	31.0	41.0
15	37.27909457599008	39.0	36.0	41.0	32.0	41.0
16	37.233660158225604	39.0	36.0	41.0	32.0	41.0
17	37.35810527440629	39.0	36.0	41.0	32.0	41.0
18	37.37691697949977	39.0	36.0	41.0	32.0	41.0
19	37.48012116137504	39.0	36.0	41.0	32.0	41.0
20	37.45407080701791	39.0	36.0	41.0	32.0	41.0
21	37.36380616814892	39.0	36.0	41.0	32.0	41.0
22	37.303244876405	39.0	36.0	41.0	31.0	41.0
23	37.20483694009341	39.0	36.0	40.0	31.0	41.0
24	37.12259227082063	39.0	36.0	40.0	31.0	41.0
25	37.032306040429575	39.0	36.0	40.0	31.0	41.0
26	36.75932991626149	39.0	35.0	40.0	30.0	41.0
27	36.60337801192584	38.0	35.0	40.0	30.0	41.0
28	36.44487977181787	38.0	35.0	40.0	30.0	41.0
29	36.31135889720752	38.0	35.0	40.0	30.0	41.0
30	36.1275411208708	38.0	35.0	40.0	30.0	41.0
31	35.85506196766834	38.0	34.0	40.0	29.0	41.0
32	35.55936131545799	38.0	34.0	40.0	28.0	41.0
33	35.65262754185279	38.0	34.0	40.0	29.0	41.0
34	35.60828624063788	38.0	34.0	40.0	28.0	41.0
35	35.46841160321077	38.0	34.0	40.0	27.0	41.0
36	35.35283401535706	38.0	34.0	40.0	27.0	41.0
37	35.20379615553801	38.0	34.0	40.0	26.0	41.0
38	35.04668636008657	38.0	34.0	40.0	25.0	41.0
39	34.950439039814036	38.0	34.0	40.0	24.0	41.0
40	34.74326106638698	38.0	33.0	40.0	24.0	41.0
41	34.58669179090331	38.0	33.0	40.0	23.0	41.0
42	34.35986480047604	38.0	33.0	40.0	22.0	41.0
43	34.00430256822006	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	4.0
16	34.0
17	82.0
18	219.0
19	517.0
20	1083.0
21	2038.0
22	3699.0
23	6459.0
24	10848.0
25	16652.0
26	25481.0
27	37239.0
28	52019.0
29	70081.0
30	89314.0
31	109906.0
32	133712.0
33	162669.0
34	196852.0
35	236594.0
36	292364.0
37	376383.0
38	445625.0
39	462677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19349663355234	24.013688293076733	14.236168800323801	21.55664627304712
2	17.346262873487586	23.202615575026147	36.88296736313354	22.56815418835273
3	20.043007384300548	26.157360476682257	32.65904351752976	21.140588621487446
4	12.510640228884773	15.508714557882481	35.20966099846517	36.77098421476758
5	10.652561669412572	41.29038255053697	34.505228441963084	13.55182733808737
6	31.300095075888713	35.74044648339978	15.44280552186709	17.51665291884442
7	23.902620039713764	36.20213909770859	21.08478002630506	18.810460836272586
8	28.038970135631352	33.35805989561414	20.202382093821385	18.400587874933123
9	24.989917857067052	13.55102223048474	24.624472197072773	36.834587715375434
10	16.316200887521344	27.9298048638746	33.567570851298825	22.186423397305234
11	30.72451633160772	21.563562879269725	25.842746383054095	21.869174406068463
12	20.214312324660373	27.65442146797465	32.11738907995963	20.01387712740535
13	31.39601266800217	20.8825882306443	29.63531553264821	18.086083568705323
14	20.603581850532503	22.8418907732473	29.36340873775962	27.191118638460576
15	22.792303464085247	28.87357395315884	28.14136518436598	20.192757398389933
16	21.18428400683024	28.639141257592716	27.869531581077627	22.307043154499418
17	18.536760847178137	27.131870038066953	28.574000733379833	25.75736838137508
18	19.69289536455638	26.49587162778851	33.80306482506842	20.008168182586694
19	18.842043011775797	24.735760025236463	35.42016004075308	21.00203692223466
20	24.18371238043237	23.243200317358777	33.9259169260699	18.647170376138952
21	23.408796312899945	24.47040387856928	32.434784454396876	19.686015354133897
22	21.34245105494713	23.931457530208004	33.45899111234398	21.267100302500882
23	20.111807488525386	26.296424517136714	34.655051647652705	18.936716346685188
24	18.48761268761752	25.37761376353404	32.994041471824524	23.140732077023912
25	20.006301796780594	26.458617103266764	34.307537929717036	19.227543170235613
26	21.739625273645096	25.041444743635445	32.616848559991865	20.602081422727604
27	20.063903586168838	26.850301951946786	32.12423249458199	20.961561967302384
28	19.652969346625902	26.253753814197268	32.33952558668557	21.75375125249126
29	18.605158397601656	26.236041446939385	33.39494846213469	21.76385169332427
30	17.882464536839894	26.385791461028766	35.97960003718133	19.752143964950005
31	19.776553363629777	25.78254629185736	34.1851615741171	20.255738770395755
32	21.09517323353903	25.977418927494206	31.89774108764182	21.029666751324953
33	22.12077053189068	25.877841755368785	31.250690745727255	20.750696967013276
34	19.928352742525856	29.18013697076069	31.402819486824413	19.488690799889042
35	18.346755452957197	30.149706099129244	31.554435886719894	19.94910256119367
36	19.50424401493987	25.941445255976642	34.2256731248495	20.328637604233986
37	19.718549020440218	26.311684965786586	32.97186441695205	20.99790159682114
38	20.13241092399272	25.820020391179828	33.59889685620119	20.44867182862626
39	21.2243564079612	25.239501213882694	33.435899162468516	20.100243215687595
40	19.117206832875034	26.278675554078713	32.38076905342035	22.2233485596259
41	20.350631680105867	25.74649942873956	32.09294308547974	21.809925805674837
42	17.43753279898586	27.72388029660164	32.608468121764474	22.230118782648027
43	18.333471177513783	27.45727989273039	32.14509210065016	22.064156829105663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	221.0
2	317.0
3	1123.5
4	1930.0
5	1930.0
6	3461.5
7	4993.0
8	5601.5
9	6210.0
10	9668.0
11	13126.0
12	13126.0
13	24115.0
14	35104.0
15	59091.0
16	83078.0
17	81335.0
18	79592.0
19	79592.0
20	84183.0
21	88774.0
22	71713.0
23	54652.0
24	58201.0
25	61750.0
26	61750.0
27	65724.5
28	69699.0
29	72017.0
30	74335.0
31	78885.0
32	83435.0
33	83435.0
34	90348.0
35	97261.0
36	105610.0
37	113959.0
38	117420.5
39	120882.0
40	120882.0
41	126763.0
42	132644.0
43	155576.0
44	178508.0
45	234717.0
46	290926.0
47	290926.0
48	292799.0
49	294672.0
50	263718.5
51	232765.0
52	214433.5
53	196102.0
54	196102.0
55	168457.5
56	140813.0
57	112590.0
58	84367.0
59	72110.5
60	59854.0
61	59854.0
62	52127.5
63	44401.0
64	38214.5
65	32028.0
66	26644.5
67	21261.0
68	21261.0
69	17577.5
70	13894.0
71	11435.5
72	8977.0
73	7192.0
74	5407.0
75	5407.0
76	4166.0
77	2925.0
78	2303.5
79	1682.0
80	1338.5
81	995.0
82	995.0
83	759.0
84	523.0
85	399.5
86	276.0
87	228.0
88	180.0
89	180.0
90	139.0
91	98.0
92	61.0
93	24.0
94	15.0
95	6.0
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2732554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.513988155269985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11666539803491	28.891310504907903
2	14.09917462043869	11.142292379021951
3	5.253443695094538	6.227535358270302
4	2.494525521331179	3.942746076115956
5	1.3498504966416176	2.666898826784109
6	0.8057743837018518	1.9103615672048964
7	0.5174496454897348	1.4312549413981028
8	0.3501998206659182	1.1070233252616613
9	0.2588754917360904	0.9206282802734611
>10	1.4835277597933623	11.167064352447472
>50	0.15327414108646137	4.217972869181157
>100	0.09644279119639242	7.085365312460628
>500	0.010960177794246823	2.879354969325115
>1k	0.00824355252900608	6.942425840383709
>5k	7.4941386627328E-4	2.29036571298863
>10k+	8.430905995574399E-4	7.177399683974954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40075	1.4665766897927726	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39226	1.4355068554912365	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34811	1.273936397963224	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21414	0.783662463761009	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13341	0.48822456939551795	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11913	0.43596576682473615	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11413	0.4176678667649386	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11149	0.4080065755333655	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10561	0.3864882450630436	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9370	0.34290264712060586	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8916	0.32628815386630966	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8726	0.3193349518435866	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8399	0.30736812520447904	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7279	0.26638082907053257	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7109	0.2601595430502014	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6155	0.2252471497361077	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5922	0.21672032830824203	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4886	0.17880707938434154	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4815	0.1762087775758503	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4756	0.17404962536879418	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4332	0.15853300611808585	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4274	0.15641044971114934	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3996	0.14623681727790191	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3846	0.14074744725996266	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3740	0.1368682924472856	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3624	0.13262317963341255	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3302	0.12083933199490293	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3160	0.11564272837792043	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3061	0.11201974416608053	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3059	0.11194655256584134	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3054	0.11176357356524336	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3026	0.1107388911618947	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2869	0.10499335054311827	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2864	0.10481037154252029	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2784	0.10188270753295268	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2778	0.10166313273223511	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.6595800119595075E-5	0.0	0.0	0.0	0.0
9	3.6595800119595075E-5	0.0	0.0	1.463832004783803E-4	0.0
10	3.6595800119595075E-5	3.6595800119595075E-5	0.0	2.927664009567606E-4	0.0
11	3.6595800119595075E-5	3.6595800119595075E-5	0.0	4.75745401554736E-4	0.0
12	3.6595800119595075E-5	3.6595800119595075E-5	0.0	9.51490803109472E-4	0.0
13	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.0012442572040662326	0.0
14	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.0014638320047838029	0.0
15	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.0021591522070561094	0.0
16	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.003769367412318293	0.0
17	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.005782136418896021	0.0
18	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.006550648221407518	0.0
19	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.007721713825234561	0.0
20	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.00995405763252986	0.0
21	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.014455341047240054	0.0
22	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.024994931481683438	0.0
23	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.0401089969310762	0.0
24	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.06104179459948458	0.0
25	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.07019074462938335	0.0
26	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.08084012246418552	0.0
27	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.1612410953269359	0.0
28	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.24244717579231737	0.0
29	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.34099966551438693	0.0
30	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.5441795477783787	0.0
31	3.6595800119595075E-5	3.6595800119595075E-5	0.0	0.8194531562779729	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8705	0.0	31.410683	1
GTATTGG	705	0.0	24.666666	1
ATTGGAC	750	0.0	22.693335	3
TATACCG	160	0.0	21.968752	5
TATTGGA	865	0.0	19.46243	2
GGACCCT	1430	0.0	18.888113	6
TTGGACC	1530	0.0	18.5	4
CAATGCG	100	2.8785325E-7	18.5	19
GTATCAA	15320	0.0	18.22226	2
TGGACCC	1580	0.0	17.094936	5
TCCGATA	65	0.0015806868	17.076923	8
CGTCTGT	890	0.0	17.044943	34
GACCCTC	1675	0.0	16.34627	7
TTTTTAC	2160	0.0	16.101852	1
ACCGACC	220	0.0	15.977273	8
CGAACGA	70	0.002593719	15.857143	16
CGCCGGA	140	3.4786353E-8	15.857143	14
TGCGACG	105	9.352443E-6	15.857142	22
CGGGATA	225	0.0	15.622222	24
CTGTGCG	135	3.977675E-7	15.074075	9
>>END_MODULE