FastQCFastQC Report
Wed 25 May 2016
SRR2088190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505676
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187401.2446236773382853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT179601.1928197035750054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150971.0026725537233774No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG89080.5916279465170462No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.41854954186690896No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT53310.3540602360667235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53210.3533960825569379No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA51630.3429024571023248No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT51240.3403122584141608No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41230.2738304920846185No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37820.2511828574009282No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT37060.24613529072655738No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT36680.24361150738937196No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30710.20396154285516935No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28320.18808827397129263No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26570.17646558755004396No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24130.16026024191127441No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.14571528004696893No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA21880.14531678794109754No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC20180.13402617827474173No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18610.1235989681711072No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG18360.1219385843966431No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17450.1158947874575938No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT17430.11576195675563666No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG17300.11489855719291535No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.11031589797539443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.030.7600961
GCGACCT454.009342E-628.77777934
GTATTGG4800.023.8958341
ATTGGAC5600.020.1517873
GTATCAA69850.018.8045832
TATTGGA6500.018.7846152
CTACGAT500.007035868718.514
TTGGACC9200.018.0978264
CGACGAG852.7240236E-517.41176424
GGACCCT8950.017.1564246
AAGACCG650.001580241417.0769235
TGGACCC10000.016.8355
GCGACGA1005.8802234E-616.65000223
CGAATTA803.3834364E-416.187515
GACCCTC9650.015.9119187
GGGTACT700.00259299415.8571431
CGGTCAG700.00259299415.85714310
ACGGACC750.004105191714.88
GCTACGA750.004105191714.813
ACGTTTA1152.2106326E-514.47826126
Produced by FastQC (version 0.11.5)