##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505676
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.356530222969617	31.0	30.0	33.0	27.0	34.0
2	30.716249711093223	31.0	31.0	34.0	27.0	34.0
3	30.908660296106202	31.0	31.0	34.0	27.0	34.0
4	34.95005964098518	35.0	35.0	37.0	32.0	37.0
5	34.778575204758525	35.0	35.0	37.0	32.0	37.0
6	34.83497711327005	35.0	35.0	37.0	32.0	37.0
7	34.65240064927647	35.0	35.0	37.0	30.0	37.0
8	34.765102186659014	35.0	35.0	37.0	32.0	37.0
9	36.22208894875126	38.0	35.0	39.0	32.0	39.0
10	36.04702273264633	38.0	35.0	39.0	31.0	39.0
11	36.10346648282898	38.0	35.0	39.0	31.0	39.0
12	35.909074727896304	37.0	35.0	39.0	30.0	39.0
13	36.00170953113419	37.0	35.0	39.0	30.0	39.0
14	36.852409150441396	38.0	36.0	40.0	31.0	41.0
15	36.921050743984765	38.0	36.0	40.0	31.0	41.0
16	36.86674291148959	38.0	36.0	40.0	31.0	41.0
17	36.961154325366145	38.0	36.0	40.0	31.0	41.0
18	36.96434956790173	38.0	36.0	40.0	31.0	41.0
19	37.07199822538182	39.0	36.0	40.0	31.0	41.0
20	37.033137939370754	39.0	36.0	40.0	31.0	41.0
21	36.93116912270634	39.0	36.0	40.0	31.0	41.0
22	36.86528044546104	39.0	36.0	40.0	31.0	41.0
23	36.739532276532266	38.0	35.0	40.0	30.0	41.0
24	36.66496045630003	38.0	35.0	40.0	30.0	41.0
25	36.56294049981537	38.0	35.0	40.0	30.0	41.0
26	36.28194777628122	38.0	35.0	40.0	30.0	41.0
27	36.12048541651723	38.0	35.0	40.0	30.0	41.0
28	35.96620786942211	38.0	34.0	40.0	29.0	41.0
29	35.83471942170826	38.0	34.0	40.0	29.0	41.0
30	35.648683382082204	38.0	34.0	40.0	28.0	41.0
31	35.394758898992876	38.0	34.0	40.0	27.0	41.0
32	35.091072714182864	37.0	33.0	40.0	27.0	41.0
33	35.27316700272834	38.0	34.0	40.0	27.0	41.0
34	35.255162465231564	38.0	34.0	40.0	27.0	41.0
35	35.16224539675203	38.0	33.0	40.0	26.0	41.0
36	35.06271933669661	38.0	33.0	40.0	26.0	41.0
37	34.93744470922031	38.0	33.0	40.0	25.0	41.0
38	34.8018730457283	38.0	33.0	40.0	25.0	41.0
39	34.75873029788613	38.0	33.0	40.0	24.0	41.0
40	34.59511940151799	38.0	33.0	40.0	24.0	41.0
41	34.467520900910955	38.0	33.0	40.0	23.0	41.0
42	34.260655678911	38.0	33.0	40.0	23.0	41.0
43	33.911243189105754	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	12.0
16	29.0
17	58.0
18	156.0
19	360.0
20	718.0
21	1434.0
22	2618.0
23	4360.0
24	7238.0
25	11141.0
26	16754.0
27	23828.0
28	32715.0
29	43076.0
30	54404.0
31	66754.0
32	80206.0
33	95833.0
34	114836.0
35	136314.0
36	167035.0
37	207986.0
38	249607.0
39	188201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.59065562577872	24.415279249984724	14.085633296937722	21.908431827298834
2	18.294506919151267	23.762283519163486	35.447599616384935	22.495609945300316
3	20.512248319027467	26.170504145646206	32.12982075825078	21.187426777075547
4	13.66575544805124	16.504413964226035	35.317757605221836	34.51207298250088
5	11.304490474710363	40.75086539202325	34.72606324335381	13.218580889912571
6	31.88740472717902	36.476373403042885	15.093220586633512	16.54300128314458
7	24.442509543885933	35.31782402057282	21.62709640055364	18.61257003498761
8	27.428942215988034	34.199854417550654	20.170873414997647	18.20032995146366
9	24.99495243332563	13.678108703333253	23.647982700129376	37.678956163211744
10	16.312008692441136	27.845632128027543	34.044376080909835	21.797983098621483
11	31.767325772609777	21.74299118801123	24.929533312611742	21.560149726767246
12	19.876321333407716	27.445213976977783	32.067058251576036	20.61140643803846
13	31.00779981881892	20.65411150871768	28.990566363547003	19.347522308916393
14	20.80852719974284	22.471966080351947	28.60502525111644	28.11448146878877
15	23.081127679527334	29.151822835722957	26.82569158304974	20.941357901699966
16	21.730438686676283	28.36699263320927	27.34180527550416	22.560763404610288
17	19.448606473105766	27.273264633294282	28.291744040550558	24.986384853049394
18	20.39881089955608	26.257641086130086	32.42297811747016	20.920569896843677
19	19.75312085734248	24.95742776002274	33.86332783414227	21.426123548492505
20	23.870009218450715	23.994538001535524	32.667253778369314	19.468199001644443
21	23.578113750899927	24.593272390607275	31.351897752238862	20.476716106253935
22	21.894551018944313	24.3137301783385	32.187668528953104	21.604050273764077
23	20.919772912631935	26.342320658627756	33.11575664352756	19.622149785212756
24	19.763680898148074	25.427183537494123	31.617691986855075	23.191443577502728
25	20.79232185410407	26.326779466498767	32.6756885279436	20.205210151453567
26	21.99510386032586	25.389791694893194	31.42907239007595	21.186032054704995
27	21.027100119813294	26.70036581575319	31.095800158865522	21.176733905568
28	20.626084230604725	26.303733339709208	31.316896862273158	21.75328556741291
29	19.883361360611445	26.079913606911447	32.034581144947516	22.002143887529588
30	19.17756542576225	26.31409413446186	33.86651577098924	20.641824668786647
31	20.666398348648713	25.667142200579672	32.71640113809345	20.95005831267816
32	21.425725056386632	26.078253223136983	30.935539916954248	21.560481803522137
33	22.31894511169734	26.072873579707718	30.371540756444283	21.23664055215066
34	20.82612726775216	28.405048629319985	30.543622930829738	20.225201172098114
35	19.521132036374357	29.381752780810743	30.661842255571585	20.43527292724331
36	20.513775872099973	25.97172300016737	32.62713890637826	20.887362221354394
37	20.55927038752029	26.21526809220576	31.905137625890294	21.32032389438365
38	20.859401358592418	25.8721663890505	32.3165807252025	20.95185152715458
39	21.737478713880012	25.42612089187847	32.126035083245	20.710365310996522
40	20.14676464259243	26.324255683161585	31.3288516254493	22.200128048796685
41	20.94780019074489	25.85775425788815	31.15816417343439	22.03628137793257
42	18.77561972163998	27.54211397405551	31.383511459304657	22.298754844999856
43	19.375283925625432	27.210767788023453	31.09035410008528	22.323594186265836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	136.0
2	203.0
3	501.0
4	799.0
5	799.0
6	1538.0
7	2277.0
8	2524.0
9	2771.0
10	4372.5
11	5974.0
12	5974.0
13	11167.0
14	16360.0
15	27264.0
16	38168.0
17	37288.5
18	36409.0
19	36409.0
20	39255.5
21	42102.0
22	34558.0
23	27014.0
24	28996.5
25	30979.0
26	30979.0
27	34121.0
28	37263.0
29	39391.0
30	41519.0
31	44539.5
32	47560.0
33	47560.0
34	52208.5
35	56857.0
36	61870.0
37	66883.0
38	70389.0
39	73895.0
40	73895.0
41	77754.5
42	81614.0
43	91956.0
44	102298.0
45	125455.5
46	148613.0
47	148613.0
48	150506.0
49	152399.0
50	138419.0
51	124439.0
52	115180.5
53	105922.0
54	105922.0
55	91895.0
56	77868.0
57	64420.0
58	50972.0
59	45103.5
60	39235.0
61	39235.0
62	34559.5
63	29884.0
64	26260.5
65	22637.0
66	19172.0
67	15707.0
68	15707.0
69	13021.5
70	10336.0
71	8571.5
72	6807.0
73	5555.0
74	4303.0
75	4303.0
76	3328.5
77	2354.0
78	1876.5
79	1399.0
80	1114.0
81	829.0
82	829.0
83	620.0
84	411.0
85	331.0
86	251.0
87	202.5
88	154.0
89	154.0
90	131.0
91	108.0
92	64.5
93	21.0
94	15.0
95	9.0
96	9.0
97	5.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1505676.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.29859812720766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56413443359111	40.019644232143236
2	11.695566735637406	11.765412222115277
3	3.756221786957037	5.667980704164414
4	1.5715266496533173	3.161823495884371
5	0.8153309050727092	2.050500076747235
6	0.4904452914448036	1.4801226370658045
7	0.33056881898912355	1.1639003725803705
8	0.2333488182868851	0.9389694747576669
9	0.17632167247182204	0.7981859650299579
>10	1.1969986837285158	11.668048923851888
>50	0.10321384552831707	3.584011217244015
>100	0.05391199042787294	4.997969923999686
>500	0.0064053513347333835	2.3619835373603832
>1k	0.004804013501050038	4.4384348789853
>5k	8.006689168416729E-4	2.42635245501678
>10k+	4.0033445842083647E-4	3.4766598830535878
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18740	1.2446236773382853	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17960	1.1928197035750054	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15097	1.0026725537233774	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8908	0.5916279465170462	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6302	0.41854954186690896	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5331	0.3540602360667235	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5321	0.3533960825569379	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5163	0.3429024571023248	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5124	0.3403122584141608	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4123	0.2738304920846185	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3782	0.2511828574009282	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3706	0.24613529072655738	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3668	0.24361150738937196	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3071	0.20396154285516935	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2832	0.18808827397129263	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2657	0.17646558755004396	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2413	0.16026024191127441	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2194	0.14571528004696893	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2188	0.14531678794109754	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2018	0.13402617827474173	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1861	0.1235989681711072	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1836	0.1219385843966431	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1745	0.1158947874575938	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1743	0.11576195675563666	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1730	0.11489855719291535	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.11031589797539443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3283070195712756E-4	0.0
10	0.0	0.0	0.0	2.656614039142551E-4	0.0
11	0.0	0.0	0.0	3.3207675489281893E-4	0.0
12	0.0	0.0	0.0	7.305688607642016E-4	0.0
13	0.0	0.0	0.0	7.969842117427654E-4	0.0
14	0.0	0.0	0.0	0.001195476317614148	0.0
15	0.0	0.0	0.0	0.0017932144764212221	0.0
16	0.0	0.0	0.0	0.00245736798620686	0.0
17	0.0	0.0	0.0	0.004250582462628082	0.0
18	0.0	0.0	0.0	0.004781905270456592	0.0
19	0.0	0.0	0.0	0.005711720184156485	0.0
20	0.0	0.0	0.0	0.007438519309599143	0.0
21	0.0	0.0	0.0	0.009364564487977492	0.0
22	0.0	0.0	0.0	0.01580685353289818	0.0
23	0.0	6.641535097856378E-5	0.0	0.02563632547772562	0.0
24	0.0	6.641535097856378E-5	0.0	0.039583549183224015	0.0
25	0.0	6.641535097856378E-5	0.0	0.045228854016401934	0.0
26	0.0	6.641535097856378E-5	0.0	0.052069635167194005	0.0
27	0.0	6.641535097856378E-5	0.0	0.10832343744603752	0.0
28	0.0	6.641535097856378E-5	0.0	0.16696819236010935	0.0
29	0.0	6.641535097856378E-5	0.0	0.23604015737781567	0.0
30	0.0	6.641535097856378E-5	0.0	0.37557880978377817	0.0
31	0.0	6.641535097856378E-5	0.0	0.5865139644916968	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4210	0.0	30.760096	1
GCGACCT	45	4.009342E-6	28.777779	34
GTATTGG	480	0.0	23.895834	1
ATTGGAC	560	0.0	20.151787	3
GTATCAA	6985	0.0	18.804583	2
TATTGGA	650	0.0	18.784615	2
CTACGAT	50	0.0070358687	18.5	14
TTGGACC	920	0.0	18.097826	4
CGACGAG	85	2.7240236E-5	17.411764	24
GGACCCT	895	0.0	17.156424	6
AAGACCG	65	0.0015802414	17.076923	5
TGGACCC	1000	0.0	16.835	5
GCGACGA	100	5.8802234E-6	16.650002	23
CGAATTA	80	3.3834364E-4	16.1875	15
GACCCTC	965	0.0	15.911918	7
GGGTACT	70	0.002592994	15.857143	1
CGGTCAG	70	0.002592994	15.857143	10
ACGGACC	75	0.0041051917	14.8	8
GCTACGA	75	0.0041051917	14.8	13
ACGTTTA	115	2.2106326E-5	14.478261	26
>>END_MODULE