FastQCFastQC Report
Wed 25 May 2016
SRR2088189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319104
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT459611.384741183162685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT441871.3312930236593972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT390371.1761306665895375No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG249460.751588380478587No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156230.4706993212626058No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA136590.4115267252848962No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT134040.4038439289639614No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA125990.379590395480226No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT125760.3788974373806907No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA106840.32189410154065673No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT100970.30420860569599506No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT97550.29390462004203544No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG97330.2932417905555234No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA83610.25190533348759186No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA80760.2433186787759588No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA71610.2157509978596633No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT69980.2108400339368697No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56700.17082923584196216No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC54000.16269451032567825No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51450.15501171400474345No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48180.14515965754613294No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46730.14079100865775823No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG46550.14024869362333933No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC46450.13994740749310655No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT43950.13241525423728812No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.12042406625402519No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36330.10945725111355355No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA35540.10707709068471492No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA35410.10668541871541234No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG34260.10322062821773587No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34210.1030699851526195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99700.030.3756281
ATTGGAC11950.023.376573
GTATTGG11750.023.1446821
TATTGGA13500.020.5555552
TTGGACC19450.020.3547554
GGACCCT20100.018.9601976
TGGACCC19700.018.8756355
CGTCTGT10400.018.534
GTATCAA179500.017.1189422
GACCCTC21800.016.6330287
ACGTTTA2003.6379788E-1215.72526
TGCGTCT11950.015.3263632
CGCCGGA1701.4897523E-915.23529414
TATACGG1954.1836756E-1115.1794862
GTACGGA750.004106577514.86
TTTTTAC25150.014.7852891
GTATACG2301.8189894E-1214.478261
GCGTCTG13300.014.46616633
ACCCTCG24850.014.2937638
GTTATCA4650.013.5268811
Produced by FastQC (version 0.11.5)