FastQCFastQC Report
Wed 25 May 2016
SRR2088188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025584
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353551.7454225546805269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT345491.7056315610707826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291031.4367708275736775No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG131160.6475169630091865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122800.6062449150467223No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT93670.46243453739760976No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86320.42614870575596964No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT77800.38408676213872145No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA73350.36211778924004134No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67140.33145996413873724No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT66200.3268193271668812No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT60500.29867929446520114No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG57530.28401685637327306No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA56730.28006737809935306No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53950.266342941097481No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT49480.24427523124195294No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.22452783987235284No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42720.2109021398273288No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40970.2022626561031288No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38380.18947622019131272No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.1837001082157047No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC36080.17812147015379268No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30480.15047512223635257No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC29550.14588385374292057No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29070.14351416677856854No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.14045332111628056No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28260.13951532002622452No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA27990.13818237110877654No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC27200.13428226131328053No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC25260.12470477649902448No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24910.12297687975418446No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA24850.12268066888364047No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24780.12233508953467247No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC24680.12184140475043247No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG24540.12115024605249647No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT22570.11142465580296843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC21910.10816633622698442No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA21800.1076232829643204No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA21720.10722833513692842No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC21260.10495738512942442No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA20900.1031801199061604No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG20330.10036611663599238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75100.029.3635161
TATGCCG751.3749741E-824.66666633
GTATTGG4650.021.4838711
ATTGGAC5200.020.6346153
TAGGTCG609.240129E-418.521
CGTCTGT9150.018.1967234
CGATCTG2800.017.83928533
GTTATCA2800.017.8392851
CCGTCTT1054.801732E-717.6190537
GTATCAA128600.017.4786172
TTTTTAC15950.017.0501561
TTGGACC12050.016.734444
TATTGGA6650.016.691732
TGCGTTA904.4486504E-516.44444537
TATCACG803.384158E-416.18752
TGGACCC12350.016.028345
ACGATCT2900.015.94827632
GGTCGTC700.002593409315.85714223
GGACCCT12450.015.7510046
ACGTTTA1302.5916052E-715.65384626
Produced by FastQC (version 0.11.5)