##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2025584
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.552462894651615	31.0	31.0	33.0	28.0	34.0
2	30.932575000592422	31.0	31.0	34.0	28.0	34.0
3	31.097498795409127	31.0	31.0	34.0	28.0	34.0
4	35.135704073491894	35.0	35.0	37.0	32.0	37.0
5	34.99943028775899	35.0	35.0	37.0	32.0	37.0
6	35.04848922582327	36.0	35.0	37.0	32.0	37.0
7	34.85124339449759	36.0	35.0	37.0	32.0	37.0
8	34.98156284804777	36.0	35.0	37.0	32.0	37.0
9	36.434728453621275	38.0	35.0	39.0	32.0	39.0
10	36.3212584617572	38.0	35.0	39.0	32.0	39.0
11	36.37125441354197	38.0	35.0	39.0	32.0	39.0
12	36.19700441946619	38.0	35.0	39.0	32.0	39.0
13	36.280116252892995	38.0	35.0	39.0	32.0	39.0
14	37.14015760393052	39.0	36.0	41.0	31.0	41.0
15	37.22055713315271	39.0	36.0	41.0	32.0	41.0
16	37.176031702462105	39.0	36.0	41.0	32.0	41.0
17	37.317125332743544	39.0	36.0	41.0	32.0	41.0
18	37.35301522918822	39.0	36.0	40.0	32.0	41.0
19	37.46988473447657	39.0	36.0	41.0	32.0	41.0
20	37.43079181115175	39.0	36.0	41.0	32.0	41.0
21	37.34207221226076	39.0	36.0	41.0	31.0	41.0
22	37.287562500493685	39.0	36.0	41.0	31.0	41.0
23	37.1724544625155	39.0	36.0	40.0	31.0	41.0
24	37.09534583606506	39.0	36.0	40.0	31.0	41.0
25	36.99838565075554	39.0	35.0	40.0	31.0	41.0
26	36.6893764958649	38.0	35.0	40.0	30.0	41.0
27	36.52406910797084	38.0	35.0	40.0	30.0	41.0
28	36.34624878553543	38.0	35.0	40.0	30.0	41.0
29	36.20478489166581	38.0	35.0	40.0	30.0	41.0
30	35.996473609586175	38.0	35.0	40.0	30.0	41.0
31	35.671021295586854	38.0	34.0	40.0	29.0	41.0
32	35.33226121454356	37.0	34.0	40.0	27.0	41.0
33	35.39324165277767	38.0	34.0	40.0	27.0	41.0
34	35.334273473724124	38.0	34.0	40.0	27.0	41.0
35	35.19549769350469	38.0	34.0	40.0	27.0	41.0
36	35.04110913198367	38.0	34.0	40.0	25.0	41.0
37	34.864587200530806	38.0	33.0	40.0	25.0	41.0
38	34.67491400011058	38.0	33.0	40.0	24.0	41.0
39	34.53143093547342	38.0	33.0	40.0	23.0	41.0
40	34.28732306337333	38.0	33.0	40.0	22.0	41.0
41	34.07492012180191	38.0	33.0	40.0	21.0	41.0
42	33.798191533898375	38.0	33.0	40.0	18.0	41.0
43	33.40898131106881	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	4.0
15	11.0
16	27.0
17	75.0
18	177.0
19	428.0
20	878.0
21	1762.0
22	3079.0
23	5388.0
24	8689.0
25	13571.0
26	20332.0
27	29688.0
28	40820.0
29	55249.0
30	69484.0
31	84693.0
32	102988.0
33	124532.0
34	150555.0
35	178767.0
36	220209.0
37	281791.0
38	317775.0
39	314607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.642098278817365	24.46879517215776	15.049437594293794	20.83966895473108
2	17.26800764618994	23.634961571576397	36.521812968506865	22.575217813726805
3	20.390860117378494	25.76022519925118	33.046222718978825	20.802691964391503
4	12.976307079834754	15.733882179164132	34.398573448447465	36.891237292553654
5	10.425832747494056	41.735914185736064	34.129367135601385	13.708885931168494
6	31.2919632066604	35.38115427452034	15.184954067567674	18.14192845125159
7	23.254972393146865	36.99278825267182	20.850826230854906	18.901413123326407
8	28.239954502010285	33.28230278280239	20.12185127844612	18.355891436741206
9	24.700530809880014	13.796663085806365	25.333039755448304	36.169766348865316
10	16.47840820227648	28.783748291850646	33.236390097868075	21.501453408004803
11	30.15490841159883	22.013009581434293	25.717521465414418	22.11456054155246
12	20.053130356479908	28.612785251068335	31.78406819959083	19.550016192860923
13	31.18596908348407	21.552056098389404	30.030154266621377	17.231820551505145
14	20.51571299931279	23.54896168216179	29.953633125064176	25.981692193461242
15	22.516814903751214	29.54737991611308	28.366041595905177	19.56976358423052
16	20.82303177750219	28.5158255594436	28.703870093760614	21.957272569293597
17	17.98681269204338	27.798057251637058	29.07131967867045	25.14381037764911
18	18.908127236392072	26.6120782944573	34.99400666671932	19.485787802431297
19	18.179399126375408	25.16266913640708	36.79847392159496	19.859457815622555
20	23.342947021698436	23.684724997827786	35.17336234883372	17.79896563164006
21	22.71596734571363	24.777298793829335	33.43282727351717	19.073906586939867
22	20.339862479166502	24.659456235831247	34.366434568993434	20.634246716008814
23	19.39643085648386	27.129706790732943	35.56283027512065	17.911032077662544
24	17.69603235412602	26.176352103887073	33.78921831926003	22.338397222726876
25	19.05618330318565	27.259891468337035	35.367429837518465	18.316495390958853
26	20.69511804990561	25.799176928727714	33.65597279599365	19.849732225373028
27	19.2979901105064	27.49690953325066	33.01704594823024	20.1880544080127
28	18.835358099195094	27.043410690447793	33.23866104787558	20.882570162481535
29	17.76193927282206	27.05590091548906	34.28443352633117	20.897726285357702
30	17.00921808229133	27.008902124029415	37.08940236494759	18.892477428731663
31	18.75503558479925	26.296909928198488	35.408405674610385	19.53964881239188
32	20.053574672785725	26.850676150680496	32.970837052425374	20.124912124108405
33	21.211956650526464	26.761812889517294	32.152998838853385	19.873231621102853
34	19.01757715305808	29.93467562934936	32.35303991342743	18.69470730416512
35	17.34206036382594	30.866851238951337	32.42111904517414	19.369969352048592
36	18.602931302774902	26.58773963459427	35.11979754974368	19.68953151288715
37	18.429351732636118	26.916928648725502	34.092143302869694	20.561576315768686
38	19.14233129803553	26.455086533068982	34.61224022306653	19.790341945828956
39	20.437365224053902	25.792215973269933	34.184758568393114	19.58566023428305
40	18.25177331574499	26.779980489577326	33.32707999273296	21.641166201944724
41	19.30623464640321	26.110938869975275	33.056392625534166	21.526433858087344
42	16.45831523155791	27.85596647682841	33.59248493274038	22.09323335887329
43	17.109090514143084	27.70405966871776	33.24167252505944	21.945177292079716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	285.5
2	409.0
3	1143.5
4	1878.0
5	1878.0
6	3358.0
7	4838.0
8	5564.5
9	6291.0
10	9624.5
11	12958.0
12	12958.0
13	23470.0
14	33982.0
15	55601.5
16	77221.0
17	74124.5
18	71028.0
19	71028.0
20	74140.0
21	77252.0
22	61001.5
23	44751.0
24	46843.0
25	48935.0
26	48935.0
27	51550.5
28	54166.0
29	54622.5
30	55079.0
31	58612.5
32	62146.0
33	62146.0
34	66100.0
35	70054.0
36	75168.5
37	80283.0
38	83302.0
39	86321.0
40	86321.0
41	90489.5
42	94658.0
43	110918.0
44	127178.0
45	169730.5
46	212283.0
47	212283.0
48	215643.0
49	219003.0
50	195820.0
51	172637.0
52	156461.0
53	140285.0
54	140285.0
55	119606.0
56	98927.0
57	77264.5
58	55602.0
59	46347.0
60	37092.0
61	37092.0
62	31983.5
63	26875.0
64	22803.0
65	18731.0
66	15779.0
67	12827.0
68	12827.0
69	10698.0
70	8569.0
71	7162.5
72	5756.0
73	4587.0
74	3418.0
75	3418.0
76	2678.0
77	1938.0
78	1515.5
79	1093.0
80	782.5
81	472.0
82	472.0
83	359.5
84	247.0
85	188.0
86	129.0
87	98.0
88	67.0
89	67.0
90	49.0
91	31.0
92	20.5
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2025584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.27632729869066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60217333400921	24.553090472307083
2	14.221294987467076	10.03350113178235
3	5.984737089884578	6.333586331381456
4	3.1201584087528094	4.4027091700370375
5	1.8424951796431093	3.249823150167509
6	1.1596092828769868	2.4544053960821133
7	0.7721739615813327	1.9067622980188776
8	0.5528643528878852	1.560241909140148
9	0.4008575326736385	1.2726703370466772
>10	2.057569685646628	13.29081983250754
>50	0.16554697414905928	4.02865385828046
>100	0.09536407221401225	6.644302172412803
>500	0.011511673419881869	2.7214517960885605
>1k	0.011511673419881869	7.83582392132818
>5k	0.0014211942493681318	3.475274341010315
>10k+	7.105971246840659E-4	6.236883882408953
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35355	1.7454225546805269	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34549	1.7056315610707826	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29103	1.4367708275736775	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13116	0.6475169630091865	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12280	0.6062449150467223	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9367	0.46243453739760976	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8632	0.42614870575596964	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7780	0.38408676213872145	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7335	0.36211778924004134	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6714	0.33145996413873724	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6620	0.3268193271668812	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6050	0.29867929446520114	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5753	0.28401685637327306	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5673	0.28006737809935306	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5395	0.266342941097481	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4948	0.24427523124195294	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4548	0.22452783987235284	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4272	0.2109021398273288	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4097	0.2022626561031288	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3838	0.18947622019131272	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3721	0.1837001082157047	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3608	0.17812147015379268	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3048	0.15047512223635257	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2955	0.14588385374292057	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2907	0.14351416677856854	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2845	0.14045332111628056	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2826	0.13951532002622452	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2799	0.13818237110877654	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2720	0.13428226131328053	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2526	0.12470477649902448	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2491	0.12297687975418446	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2485	0.12268066888364047	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2478	0.12233508953467247	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2468	0.12184140475043247	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2454	0.12115024605249647	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	2257	0.11142465580296843	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2191	0.10816633622698442	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2180	0.1076232829643204	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2172	0.10722833513692842	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2126	0.10495738512942442	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2090	0.1031801199061604	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	2033	0.10036611663599238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.936847842400019E-5	0.0	0.0	0.0	0.0
2	4.936847842400019E-5	0.0	0.0	0.0	0.0
3	4.936847842400019E-5	0.0	0.0	0.0	0.0
4	4.936847842400019E-5	0.0	0.0	0.0	0.0
5	4.936847842400019E-5	0.0	0.0	0.0	0.0
6	1.9747391369600076E-4	0.0	0.0	0.0	0.0
7	2.4684239212000096E-4	0.0	0.0	0.0	0.0
8	4.443163058160017E-4	0.0	0.0	4.936847842400019E-5	0.0
9	4.443163058160017E-4	0.0	0.0	4.936847842400019E-5	0.0
10	4.443163058160017E-4	0.0	0.0	4.936847842400019E-5	0.0
11	4.443163058160017E-4	0.0	0.0	1.4810543527200056E-4	0.0
12	4.443163058160017E-4	0.0	0.0	2.4684239212000096E-4	0.0
13	4.936847842400019E-4	0.0	0.0	3.4557934896800135E-4	0.0
14	4.936847842400019E-4	0.0	0.0	6.417902195120025E-4	0.0
15	4.936847842400019E-4	0.0	0.0	0.001086106525328004	0.0
16	4.936847842400019E-4	0.0	0.0	0.002172213050656008	0.0
17	4.936847842400019E-4	0.0	0.0	0.003406425011256013	0.0
18	4.936847842400019E-4	0.0	0.0	0.004295057622888017	0.0
19	4.936847842400019E-4	0.0	0.0	0.004986216320824019	0.0
20	4.936847842400019E-4	0.0	0.0	0.0061216913245760235	0.0
21	4.936847842400019E-4	0.0	0.0	0.008935694594744035	0.0
22	4.936847842400019E-4	0.0	0.0	0.013971279393992054	0.0
23	4.936847842400019E-4	0.0	0.0	0.024536133776728093	0.0
24	4.936847842400019E-4	0.0	0.0	0.039297308825504154	0.0
25	4.936847842400019E-4	0.0	0.0	0.04778868711443218	0.0
26	4.936847842400019E-4	0.0	0.0	0.06185870346527224	0.0
27	4.936847842400019E-4	0.0	0.0	0.16696419402996865	0.0
28	4.936847842400019E-4	0.0	0.0	0.2745381085158651	0.0
29	4.936847842400019E-4	0.0	0.0	0.3921831926002575	0.0
30	4.936847842400019E-4	0.0	0.0	0.6310278912155705	0.0
31	4.936847842400019E-4	0.0	0.0	0.9640676466638757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7510	0.0	29.363516	1
TATGCCG	75	1.3749741E-8	24.666666	33
GTATTGG	465	0.0	21.483871	1
ATTGGAC	520	0.0	20.634615	3
TAGGTCG	60	9.240129E-4	18.5	21
CGTCTGT	915	0.0	18.19672	34
CGATCTG	280	0.0	17.839285	33
GTTATCA	280	0.0	17.839285	1
CCGTCTT	105	4.801732E-7	17.61905	37
GTATCAA	12860	0.0	17.478617	2
TTTTTAC	1595	0.0	17.050156	1
TTGGACC	1205	0.0	16.73444	4
TATTGGA	665	0.0	16.69173	2
TGCGTTA	90	4.4486504E-5	16.444445	37
TATCACG	80	3.384158E-4	16.1875	2
TGGACCC	1235	0.0	16.02834	5
ACGATCT	290	0.0	15.948276	32
GGTCGTC	70	0.0025934093	15.857142	23
GGACCCT	1245	0.0	15.751004	6
ACGTTTA	130	2.5916052E-7	15.653846	26
>>END_MODULE