FastQCFastQC Report
Wed 25 May 2016
SRR2088187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974257
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324661.6444667538218174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319601.618836858625802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT269791.3665394120421No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG129600.6564494896054566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109280.5535246930870702No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT89480.45323379884179216No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT77640.3932618701617875No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76540.3876901538148275No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA66480.33673427522353977No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT66130.33496145638587077No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62740.31779043964387615No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT57600.29175532871353627No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG57090.28917207840721854No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51260.2596419817683311No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50780.25721068736238495No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47070.23841880768309293No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.20848349530988114No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38100.19298399347197454No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38010.19252812577085962No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36200.18336011978177105No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.17176081938673637No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC33800.1712036477520404No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29620.15003112563359278No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC27800.1408124676777137No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26550.13448097182889562No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA26000.1316951136554157No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25570.12951707908342228No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC25040.1268325248435234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24840.12581948550771252No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC24040.12176732816446896No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23900.12105820062940134No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC23730.12019711719396207No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA23110.11705669525294833No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA22770.11533452838206981No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG22580.11437214101304946No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21570.10925629236720447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC21400.10839520893176523No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT21220.10748347352953541No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT21180.10728086566237323No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC20440.10353262011987294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68400.031.6447371
TATACCG1103.6379788E-1223.5454545
CGTTTAT1251.8189894E-1120.7227
ACGTTTA1303.274181E-1119.92307726
CGATCTG2700.019.18518633
CGCCGGA1205.180482E-918.514
ACACCGC500.007036628718.4999986
CGGTAAG500.007036628718.4999981
GTATCAA119600.018.329852
CCGCCGG1156.411756E-817.69565213
GTAATAC953.6090387E-617.5263163
ACGATCT2850.017.52631632
TTCCCGC650.001580476817.07692335
GTATTGG3800.017.0394741
TTTTTAC16800.016.9583321
GTTATCA2750.016.8181821
ATTGGAC3800.016.5526313
TTGGACC9050.016.353594
CGACGAC1251.6593731E-716.27999925
GGACCCT8550.016.228076
Produced by FastQC (version 0.11.5)