##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1974257
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.538366585505333	31.0	31.0	33.0	28.0	34.0
2	30.92554363489657	31.0	31.0	34.0	27.0	34.0
3	31.104576557155426	31.0	31.0	34.0	28.0	34.0
4	35.13864861565642	35.0	35.0	37.0	32.0	37.0
5	34.9959787403565	35.0	35.0	37.0	32.0	37.0
6	35.045285390909086	36.0	35.0	37.0	32.0	37.0
7	34.85701506946664	36.0	35.0	37.0	32.0	37.0
8	34.97930461940872	36.0	35.0	37.0	32.0	37.0
9	36.436700996881356	38.0	35.0	39.0	32.0	39.0
10	36.31819869449621	38.0	35.0	39.0	32.0	39.0
11	36.37198044631474	38.0	35.0	39.0	32.0	39.0
12	36.20605321394327	38.0	35.0	39.0	32.0	39.0
13	36.285721666429446	38.0	35.0	39.0	32.0	39.0
14	37.15751242112856	39.0	36.0	41.0	31.0	41.0
15	37.23037274275841	39.0	36.0	41.0	32.0	41.0
16	37.19034603904152	39.0	36.0	41.0	32.0	41.0
17	37.32369949808966	39.0	36.0	41.0	32.0	41.0
18	37.35892186275647	39.0	36.0	40.0	32.0	41.0
19	37.46828553729327	39.0	36.0	41.0	32.0	41.0
20	37.43830210555161	39.0	36.0	41.0	32.0	41.0
21	37.33942693377813	39.0	36.0	41.0	31.0	41.0
22	37.286734199245586	39.0	36.0	41.0	31.0	41.0
23	37.180754076090395	39.0	36.0	40.0	31.0	41.0
24	37.10477865850292	39.0	36.0	40.0	31.0	41.0
25	37.01171225428098	39.0	35.0	40.0	31.0	41.0
26	36.714402937408856	38.0	35.0	40.0	30.0	41.0
27	36.53951739819081	38.0	35.0	40.0	30.0	41.0
28	36.37752430407996	38.0	35.0	40.0	30.0	41.0
29	36.231459227446074	38.0	35.0	40.0	30.0	41.0
30	36.03671254552979	38.0	35.0	40.0	30.0	41.0
31	35.7344140099288	38.0	34.0	40.0	29.0	41.0
32	35.41370196484044	38.0	34.0	40.0	27.0	41.0
33	35.47884393977076	38.0	34.0	40.0	28.0	41.0
34	35.427589214575406	38.0	34.0	40.0	27.0	41.0
35	35.29541037463714	38.0	34.0	40.0	27.0	41.0
36	35.15618787219698	38.0	34.0	40.0	26.0	41.0
37	34.98953732973975	38.0	34.0	40.0	25.0	41.0
38	34.81161419207327	38.0	33.0	40.0	24.0	41.0
39	34.69428853487666	38.0	33.0	40.0	24.0	41.0
40	34.46110156884337	38.0	33.0	40.0	23.0	41.0
41	34.279924042310604	38.0	33.0	40.0	21.0	41.0
42	34.02615768868997	38.0	33.0	40.0	19.0	41.0
43	33.64230036920219	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	6.0
15	5.0
16	24.0
17	50.0
18	165.0
19	407.0
20	829.0
21	1680.0
22	2938.0
23	5035.0
24	8326.0
25	13137.0
26	19618.0
27	28412.0
28	39458.0
29	52628.0
30	67049.0
31	81616.0
32	99313.0
33	120012.0
34	144823.0
35	172667.0
36	211878.0
37	270408.0
38	311458.0
39	322313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.39026175416878	24.684830799637535	14.930629598882009	20.994277847311672
2	17.24709599611398	23.745439423540095	36.694564081576004	22.312900498769917
3	20.085834822923257	25.96804772630919	33.26157638038006	20.684541070387493
4	12.868638682805733	15.952127813146921	34.87306870382124	36.30616480022611
5	10.361822194374897	41.688392139422575	34.46005256661114	13.489733099591392
6	30.903119502678727	35.96061708278101	15.35580220812184	17.780461206418416
7	23.078150413041463	37.14491071831073	21.173028638115504	18.603910230532296
8	27.811120841916733	33.810238484655244	20.266966256166246	18.111674417261785
9	24.782538443576495	13.876967385705102	25.374761239291544	35.96573293142686
10	16.233600792601976	28.875521272053234	33.62738488454137	21.26349305080342
11	30.029525031442205	22.138759036944027	25.97265705528713	21.85905887632664
12	19.83414519994104	28.660858236794905	32.17787755089636	19.327119012367692
13	30.8829093679293	21.658679695703245	30.208681037980362	17.249729898387088
14	20.139576559688024	23.630965978593466	30.10281842738813	26.126639034330385
15	22.253029874023493	29.721003901720998	28.5762694522547	19.449696772000806
16	20.502497901742274	28.870810639141713	28.889602518821007	21.737088940295006
17	17.903798745553388	28.030747769920534	29.31685185869925	24.74860162582683
18	18.847242278994074	27.023736018157717	34.81446437824457	19.314557324603634
19	18.160908128982197	25.53664492515412	36.60546727199144	19.696979673872246
20	23.210706610132316	23.964610483842783	35.063975966654795	17.760706939370102
21	22.35489097923928	25.1515886736124	33.53479308924826	18.95872725790006
22	20.275577090520635	24.915702464268836	34.327952237221396	20.480768207989133
23	19.283811580761775	27.25384790328716	35.49968418498706	17.962656330964002
24	17.565646215259715	26.39068773720949	33.823256040120405	22.220410007410383
25	18.96237419950898	27.417605711920988	35.32123730598397	18.298782782586056
26	20.610487894939716	25.997476519014494	33.59192850778799	19.800107078257795
27	19.216950984598256	27.749274790465478	33.0170793366821	20.016694888254165
28	18.82642432064316	27.135727516731613	33.172834134563026	20.865014028062205
29	17.648259573095093	27.15330374920793	34.443337417570255	20.755099260126723
30	17.04509595255329	27.015986267238763	37.05677629609519	18.882141484112758
31	18.74659682098126	26.553179246673558	35.19172022690055	19.508503705444628
32	20.139880471488766	26.954646735455412	32.97594993964818	19.929522853407637
33	21.166798446200268	26.8025895311502	32.236937744174135	19.793674278475397
34	19.070414844673213	29.87503653273105	32.35870507233861	18.695843550257134
35	17.418704859600346	30.66323178795871	32.53234001449659	19.38572333794435
36	18.532187045556885	26.526890875909263	35.23239375623336	19.70852832230049
37	18.579090766804928	26.750924525023844	34.196459731433144	20.473524976738084
38	19.133932411028553	26.330361244761953	34.82869758091272	19.707008763296773
39	20.421353450943826	25.699541650352515	34.377439208775755	19.501665689927908
40	18.273558103124365	26.631436535364948	33.55160954222272	21.543395819287962
41	19.225105951251535	25.972302491519596	33.30240186561324	21.50018969161563
42	16.394370135195164	27.94403160277512	33.741149201952936	21.920449060076777
43	17.151819646580968	27.72009925759412	33.339783017104665	21.788298078720246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	309.0
2	357.0
3	1080.5
4	1804.0
5	1804.0
6	3165.5
7	4527.0
8	5111.5
9	5696.0
10	8641.0
11	11586.0
12	11586.0
13	21083.0
14	30580.0
15	50379.0
16	70178.0
17	68484.0
18	66790.0
19	66790.0
20	70743.5
21	74697.0
22	60366.0
23	46035.0
24	49116.5
25	52198.0
26	52198.0
27	55125.5
28	58053.0
29	58505.0
30	58957.0
31	62953.0
32	66949.0
33	66949.0
34	70634.0
35	74319.0
36	78652.0
37	82985.0
38	84890.0
39	86795.0
40	86795.0
41	91176.5
42	95558.0
43	111299.5
44	127041.0
45	167910.5
46	208780.0
47	208780.0
48	209649.5
49	210519.0
50	185894.0
51	161269.0
52	144559.0
53	127849.0
54	127849.0
55	108980.5
56	90112.0
57	70023.5
58	49935.0
59	42211.0
60	34487.0
61	34487.0
62	29684.0
63	24881.0
64	21262.0
65	17643.0
66	14787.0
67	11931.0
68	11931.0
69	10259.0
70	8587.0
71	7017.0
72	5447.0
73	4423.0
74	3399.0
75	3399.0
76	2647.5
77	1896.0
78	1463.5
79	1031.0
80	794.5
81	558.0
82	558.0
83	434.0
84	310.0
85	224.5
86	139.0
87	108.0
88	77.0
89	77.0
90	54.0
91	31.0
92	19.0
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1974257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48167793952348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00396061372676	25.9034362354059
2	13.989521065185825	10.207224040565773
3	5.73456564577387	6.276197310365333
4	2.885250108187493	4.2103506088748555
5	1.6572297588973333	3.0229261167943324
6	1.0415458161494604	2.2798403414413646
7	0.6680941451784215	1.706123680637629
8	0.4823695831014533	1.407812142282354
9	0.3699738673564816	1.2147540727445223
>10	1.8773400266501963	12.579702405688773
>50	0.1595139072242448	4.08423393369897
>100	0.10608872518581493	7.699979152794177
>500	0.012414441894677676	3.097216159183366
>1k	0.010016197437751307	7.001725981925228
>5k	0.0014107320334860998	3.3748230711580263
>10k+	7.053660167430499E-4	5.933654746439478
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32466	1.6444667538218174	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31960	1.618836858625802	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26979	1.3665394120421	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12960	0.6564494896054566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10928	0.5535246930870702	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8948	0.45323379884179216	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7764	0.3932618701617875	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7654	0.3876901538148275	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6648	0.33673427522353977	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6613	0.33496145638587077	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6274	0.31779043964387615	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5760	0.29175532871353627	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5709	0.28917207840721854	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5126	0.2596419817683311	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5078	0.25721068736238495	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4707	0.23841880768309293	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4116	0.20848349530988114	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3810	0.19298399347197454	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3801	0.19252812577085962	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3620	0.18336011978177105	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3391	0.17176081938673637	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3380	0.1712036477520404	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2962	0.15003112563359278	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2780	0.1408124676777137	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2655	0.13448097182889562	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2600	0.1316951136554157	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2557	0.12951707908342228	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2504	0.1268325248435234	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2484	0.12581948550771252	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2404	0.12176732816446896	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2390	0.12105820062940134	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2373	0.12019711719396207	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2311	0.11705669525294833	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2277	0.11533452838206981	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2258	0.11437214101304946	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2157	0.10925629236720447	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2140	0.10839520893176523	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	2122	0.10748347352953541	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	2118	0.10728086566237323	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2044	0.10353262011987294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0130393358108898E-4	0.0
10	0.0	0.0	0.0	2.0260786716217797E-4	0.0
11	0.0	0.0	0.0	3.545637675338115E-4	0.0
12	0.0	0.0	0.0	5.571716346959894E-4	0.0
13	0.0	0.0	0.0	7.597795018581674E-4	0.0
14	0.0	0.0	0.0	0.0011649952361825232	0.0
15	0.0	0.0	0.0	0.0017728188376690572	0.0
16	0.0	0.0	0.0	0.003191073907804303	0.0
17	0.0	0.0	0.0	0.0043560691439868265	0.0
18	0.0	0.0	0.0	0.00552106438016935	0.0
19	0.0	0.0	0.0	0.006939319450304596	0.0
20	0.0	0.0	0.0	0.008661486321183109	0.0
21	0.0	0.0	0.0	0.01251103579726449	0.0
22	0.0	0.0	0.0	0.022692081122163935	0.0
23	0.0	0.0	0.0	0.03646941608919203	0.0
24	0.0	0.0	0.0	0.055362599702065134	0.0
25	0.0	0.0	0.0	0.06620212059524165	0.0
26	0.0	0.0	0.0	0.08033401932980357	0.0
27	0.0	0.0	0.0	0.19359181707346104	0.0
28	0.0	0.0	0.0	0.3032026732081993	0.0
29	0.0	0.0	0.0	0.4259830407084792	0.0
30	0.0	0.0	0.0	0.678989614827249	0.0
31	0.0	0.0	0.0	1.022713861467884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6840	0.0	31.644737	1
TATACCG	110	3.6379788E-12	23.545454	5
CGTTTAT	125	1.8189894E-11	20.72	27
ACGTTTA	130	3.274181E-11	19.923077	26
CGATCTG	270	0.0	19.185186	33
CGCCGGA	120	5.180482E-9	18.5	14
ACACCGC	50	0.0070366287	18.499998	6
CGGTAAG	50	0.0070366287	18.499998	1
GTATCAA	11960	0.0	18.32985	2
CCGCCGG	115	6.411756E-8	17.695652	13
GTAATAC	95	3.6090387E-6	17.526316	3
ACGATCT	285	0.0	17.526316	32
TTCCCGC	65	0.0015804768	17.076923	35
GTATTGG	380	0.0	17.039474	1
TTTTTAC	1680	0.0	16.958332	1
GTTATCA	275	0.0	16.818182	1
ATTGGAC	380	0.0	16.552631	3
TTGGACC	905	0.0	16.35359	4
CGACGAC	125	1.6593731E-7	16.279999	25
GGACCCT	855	0.0	16.22807	6
>>END_MODULE