FastQCFastQC Report
Wed 25 May 2016
SRR2088186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869021
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159371.8339027480348578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158451.823316122395201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130231.4985828881005177No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG66690.7674152868572797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52080.5992950688188203No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT44360.5104594710599629No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42560.4897465078519392No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT39660.45637562268345644No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33990.3911297885781816No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA32800.3774362184573215No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT31100.3578739754275213No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT29810.3430296851284377No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG28650.3296813310610445No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27180.3127657444411585No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25060.2883704766628194No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22910.2636299928310133No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21140.2432622456764566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.232330403983333No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18570.2136887370961116No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18230.20977628849015156No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17450.20080067110000793No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.1913647656385749No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15610.1796274198206948No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14010.16121589696911812No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13390.15408143186413217No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC13000.14959362316906036No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12740.14660175070567916No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.14280437411754146No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC12070.13889192551158142No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11680.1344041168165096No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11570.1331383246204637No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA11540.13279310856699666No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11370.13083688426401663No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC11240.12934094803232601No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA10630.12232155494516243No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG10600.12197633889169537No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA10040.11553230589364354No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC10010.11518708984017648No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT9980.1148418737867094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9960.1146117297510647No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG9810.1128856494837294No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA9540.10977870500252583No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG9350.1075923366639011No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT9180.10563611236092108No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA9010.10367988805794107No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8990.10344974402229634No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT8940.1028743839331846No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT8820.10149351971931633No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.10126337568367164No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA8780.10103323164802691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.029.5583671
CTGTGCG652.9885996E-928.4615389
GTATTGG2150.024.9534871
GTTCGCT701.9217987E-723.78571537
TAGGTCG551.9019659E-523.54545421
ATTGGAC2400.022.3541683
CGGGATA502.7013663E-422.224
CGTCATT603.724939E-521.58333426
TATACCG705.0995586E-621.1428595
TCGGGAT555.141705E-420.18181823
CGTCTGT3500.020.08571434
TGTGCGC851.2445926E-619.58823610
AGTATCA1254.110916E-1019.2400021
AGCCGTC609.234342E-418.512
ACTGCGT609.234342E-418.58
GCGCCAT500.00703353318.511
GCGCCAA801.6159309E-518.513
AAGCCGT701.2187629E-418.511
TAAGCCG609.234342E-418.510
ATTGATC500.00703353318.58
Produced by FastQC (version 0.11.5)