##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869021
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.482561411059113	31.0	31.0	33.0	27.0	34.0
2	30.87614683649762	31.0	31.0	34.0	27.0	34.0
3	31.05115411480275	31.0	31.0	34.0	28.0	34.0
4	35.10412751820727	35.0	35.0	37.0	32.0	37.0
5	34.97529864065425	35.0	35.0	37.0	32.0	37.0
6	35.029826667019556	36.0	35.0	37.0	32.0	37.0
7	34.82350023762372	36.0	35.0	37.0	32.0	37.0
8	34.96349340234586	36.0	35.0	37.0	32.0	37.0
9	36.3900297000878	38.0	35.0	39.0	32.0	39.0
10	36.28039713654791	38.0	35.0	39.0	32.0	39.0
11	36.329169260581736	38.0	35.0	39.0	32.0	39.0
12	36.14922309127167	38.0	35.0	39.0	31.0	39.0
13	36.22714065597955	38.0	35.0	39.0	32.0	39.0
14	37.045676686754405	39.0	36.0	40.0	31.0	41.0
15	37.12245733992619	39.0	36.0	40.0	31.0	41.0
16	37.07838360638005	39.0	36.0	40.0	31.0	41.0
17	37.247079184507626	39.0	36.0	40.0	32.0	41.0
18	37.28661217623049	39.0	36.0	40.0	32.0	41.0
19	37.410284676664894	39.0	36.0	41.0	32.0	41.0
20	37.39233574332496	39.0	36.0	41.0	32.0	41.0
21	37.286234740012034	39.0	36.0	41.0	31.0	41.0
22	37.23737285980431	39.0	36.0	40.0	31.0	41.0
23	37.10174322599799	39.0	36.0	40.0	31.0	41.0
24	37.024624261093805	39.0	35.0	40.0	31.0	41.0
25	36.92156230977157	39.0	35.0	40.0	31.0	41.0
26	36.623348572704224	38.0	35.0	40.0	30.0	41.0
27	36.45735143339459	38.0	35.0	40.0	30.0	41.0
28	36.27422812567245	38.0	35.0	40.0	30.0	41.0
29	36.12106611923072	38.0	35.0	40.0	30.0	41.0
30	35.91586854632972	38.0	35.0	40.0	30.0	41.0
31	35.577437139033464	38.0	34.0	40.0	29.0	41.0
32	35.23190003463668	37.0	34.0	40.0	27.0	41.0
33	35.28817945711324	37.0	34.0	40.0	27.0	41.0
34	35.21916846658481	38.0	34.0	40.0	27.0	41.0
35	35.07499013257447	38.0	33.0	40.0	26.0	41.0
36	34.915897314334174	37.0	33.0	40.0	25.0	41.0
37	34.73976002881403	37.0	33.0	40.0	25.0	41.0
38	34.554866913457786	37.0	33.0	40.0	24.0	41.0
39	34.41229613553642	38.0	33.0	40.0	23.0	41.0
40	34.140796367406544	37.0	33.0	40.0	21.0	41.0
41	33.93650671272616	37.0	33.0	40.0	20.0	41.0
42	33.653864521110535	37.0	33.0	40.0	18.0	41.0
43	33.26741816365772	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	3.0
16	18.0
17	32.0
18	64.0
19	186.0
20	412.0
21	756.0
22	1406.0
23	2430.0
24	3850.0
25	6028.0
26	9090.0
27	13166.0
28	18129.0
29	24084.0
30	30707.0
31	37230.0
32	45059.0
33	54294.0
34	65546.0
35	77234.0
36	95359.0
37	122186.0
38	135496.0
39	126252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.589607155638355	24.703315570049515	15.177193646643753	20.529883627668376
2	17.345265534434727	23.607599816345058	36.74238021865985	22.304754430560365
3	20.349450703722923	25.7986861077005	33.36271505521731	20.489148133359265
4	12.81016224003793	15.569934443471448	34.00378126650565	37.61612204998499
5	10.35694189208316	41.88586927128343	33.9644266364104	13.792762200223011
6	31.30787403296353	35.053007925009865	15.062351772857044	18.57676626916956
7	23.192880264113295	37.31624437153993	20.398701527350894	19.092173836995883
8	28.562946119829096	33.165711760705435	19.852914946819467	18.418427172646002
9	24.86153959455525	13.672166725545182	25.407671391140145	36.058622288759416
10	16.751724066507023	28.661677911120677	32.76008289788164	21.826515124490662
11	30.214459719615522	21.652756377578907	25.805360284734203	22.32742361807137
12	20.355204304614045	28.502993598543647	31.591986844966925	19.549815251875387
13	31.79025593167484	21.168303182546797	30.106982454969444	16.93445843080892
14	20.534716652416915	23.49126200632666	29.88052072389505	26.093500617361375
15	22.65491858079379	29.40055533755801	28.428542003012584	19.515984078635615
16	20.939540011115955	28.404031663216422	28.539471428193337	22.116956897474285
17	17.977471200350738	27.621196725970947	28.713690463176377	25.68764161050193
18	19.146027541336746	26.404425209517374	34.94645123650636	19.50309601263951
19	18.18333504023493	24.71079525120797	36.96769122955602	20.13817847900108
20	24.002411909493556	23.02257367773621	35.21537454215721	17.75963987061302
21	23.293683351725676	24.183765409581586	33.4913655711427	19.031185667550034
22	20.62148095385497	24.094239379715795	34.40860462520469	20.87567504122455
23	19.61149385342817	26.797281078362893	35.77635062904119	17.814874439167752
24	17.69531461265033	25.72929768095362	33.769839854272796	22.80554785212325
25	19.21760233642225	26.933641419482385	35.45299825896037	18.395757985135
26	20.95530487755762	25.474183017441465	33.583538257418404	19.986973847582508
27	19.46604282290071	27.344793739161656	32.94995172728852	20.239211710649112
28	19.064326408682874	26.771504946370683	33.09563290185162	21.068535743094817
29	17.66125329537491	26.745153454289365	34.34922746400835	21.244365786327375
30	16.870593460917515	26.675880099560313	37.48436458957839	18.969161849943788
31	18.840741478054042	26.119737037424873	35.35553225986484	19.68398922465625
32	20.39421371865582	26.50120077650598	32.83292348516319	20.271662019675013
33	21.708336162187106	26.41144460260454	31.892670027536735	19.98754920767162
34	19.19700444523205	30.057386415288008	32.18679410509067	18.558815034389273
35	17.402341255274614	31.05609645796822	32.274594054689125	19.26696823206804
36	18.632000837724288	26.37335576470534	35.34816765072421	19.646475746846164
37	18.66640737105317	26.63951734192845	34.173052204722325	20.521023082296054
38	19.28411396272357	26.23204732681949	34.75554675893908	19.728291951517857
39	20.783042066877556	25.55404299781018	34.16867946804508	19.494235467267192
40	18.410717347451904	26.692910758198018	33.26283254374751	21.633539350602575
41	19.537042257897106	25.993848249927215	33.06145651255839	21.407652979617296
42	16.376474216388324	28.050645496483973	33.61725435864035	21.955625928487343
43	17.128124636803943	27.862617819362246	33.16214452815294	21.847113015680865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	72.0
1	109.5
2	147.0
3	455.0
4	763.0
5	763.0
6	1383.0
7	2003.0
8	2268.0
9	2533.0
10	3994.0
11	5455.0
12	5455.0
13	9970.0
14	14485.0
15	24348.0
16	34211.0
17	32554.0
18	30897.0
19	30897.0
20	32408.0
21	33919.0
22	26415.5
23	18912.0
24	19720.5
25	20529.0
26	20529.0
27	21577.0
28	22625.0
29	22627.0
30	22629.0
31	23738.5
32	24848.0
33	24848.0
34	26296.0
35	27744.0
36	29562.5
37	31381.0
38	32902.0
39	34423.0
40	34423.0
41	36255.0
42	38087.0
43	46530.0
44	54973.0
45	75258.5
46	95544.0
47	95544.0
48	96784.5
49	98025.0
50	86794.5
51	75564.0
52	68263.5
53	60963.0
54	60963.0
55	51562.5
56	42162.0
57	32445.0
58	22728.0
59	19223.0
60	15718.0
61	15718.0
62	13837.5
63	11957.0
64	10299.5
65	8642.0
66	7386.0
67	6130.0
68	6130.0
69	5206.5
70	4283.0
71	3546.0
72	2809.0
73	2270.0
74	1731.0
75	1731.0
76	1355.0
77	979.0
78	767.5
79	556.0
80	438.5
81	321.0
82	321.0
83	232.5
84	144.0
85	102.5
86	61.0
87	54.0
88	47.0
89	47.0
90	31.5
91	16.0
92	10.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	869021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.40714289568081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69273981195533	33.724198589744944
2	12.153394416509418	10.550882562099872
3	4.083061035357477	5.317020414406453
4	1.803950984056702	3.1321743256701318
5	1.023072968459698	2.220433726731923
6	0.6341969479941599	1.651720652735229
7	0.4316253678645848	1.3114936814209177
8	0.3065091239988609	1.06437482753988
9	0.22129480002544707	0.8645197506098122
>10	1.4375085213732326	11.68726760982402
>50	0.11259310630823095	3.3484090411692415
>100	0.07764106289123406	6.973138824685873
>500	0.01227315770781938	3.9206389076793404
>1k	0.00880465661647912	7.669178757171475
>5k	5.336155525138861E-4	1.3755185155379888
>10k+	8.004233287708292E-4	5.1890298129729375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15937	1.8339027480348578	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15845	1.823316122395201	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13023	1.4985828881005177	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6669	0.7674152868572797	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5208	0.5992950688188203	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4436	0.5104594710599629	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4256	0.4897465078519392	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3966	0.45637562268345644	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3399	0.3911297885781816	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3280	0.3774362184573215	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3110	0.3578739754275213	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2981	0.3430296851284377	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2865	0.3296813310610445	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2718	0.3127657444411585	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2506	0.2883704766628194	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2291	0.2636299928310133	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2114	0.2432622456764566	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.232330403983333	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1857	0.2136887370961116	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1823	0.20977628849015156	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1745	0.20080067110000793	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1663	0.1913647656385749	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1561	0.1796274198206948	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1401	0.16121589696911812	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1339	0.15408143186413217	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1300	0.14959362316906036	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1274	0.14660175070567916	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1241	0.14280437411754146	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1207	0.13889192551158142	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1168	0.1344041168165096	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1157	0.1331383246204637	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1154	0.13279310856699666	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1137	0.13083688426401663	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1124	0.12934094803232601	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1063	0.12232155494516243	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1060	0.12197633889169537	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1004	0.11553230589364354	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1001	0.11518708984017648	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	998	0.1148418737867094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	996	0.1146117297510647	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	981	0.1128856494837294	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	954	0.10977870500252583	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	935	0.1075923366639011	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	918	0.10563611236092108	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	901	0.10367988805794107	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	899	0.10344974402229634	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	894	0.1028743839331846	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	882	0.10149351971931633	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	880	0.10126337568367164	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	878	0.10103323164802691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1507201782235412E-4	0.0	0.0	0.0	0.0
7	2.3014403564470824E-4	0.0	0.0	0.0	0.0
8	3.4521605346706234E-4	0.0	0.0	0.0	0.0
9	3.4521605346706234E-4	0.0	0.0	1.1507201782235412E-4	0.0
10	3.4521605346706234E-4	0.0	0.0	3.4521605346706234E-4	0.0
11	3.4521605346706234E-4	0.0	0.0	4.602880712894165E-4	0.0
12	3.4521605346706234E-4	0.0	0.0	8.055041247564789E-4	0.0
13	4.602880712894165E-4	0.0	0.0	0.0011507201782235412	0.0
14	5.753600891117706E-4	0.0	0.0	0.002071296320802374	0.0
15	5.753600891117706E-4	0.0	0.0	0.0033370885168482697	0.0
16	9.20576142578833E-4	0.0	0.0	0.005063168784183582	0.0
17	9.20576142578833E-4	0.0	0.0	0.006559105015874185	0.0
18	9.20576142578833E-4	0.0	0.0	0.007479681158453018	0.0
19	0.001035648160401187	0.0	0.0	0.008515329318854205	0.0
20	0.001035648160401187	0.0	0.0	0.010471553621834225	0.0
21	0.001035648160401187	0.0	0.0	0.015534722406017807	0.0
22	0.001035648160401187	0.0	0.0	0.024050051724872012	0.0
23	0.001035648160401187	0.0	0.0	0.03739840579226509	0.0
24	0.001035648160401187	0.0	0.0	0.057881224964644123	0.0
25	0.001035648160401187	0.0	0.0	0.06777741849736657	0.0
26	0.0011507201782235412	0.0	0.0	0.08112577256475965	0.0
27	0.0011507201782235412	0.0	0.0	0.17675061937513592	0.0
28	0.0011507201782235412	0.0	0.0	0.27156996206075573	0.0
29	0.0011507201782235412	0.0	0.0	0.3879077720791557	0.0
30	0.0012657921960458954	0.0	0.0	0.6341618902189936	0.0
31	0.00195622430298002	0.0	0.0	0.9762709992048524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3555	0.0	29.558367	1
CTGTGCG	65	2.9885996E-9	28.461538	9
GTATTGG	215	0.0	24.953487	1
GTTCGCT	70	1.9217987E-7	23.785715	37
TAGGTCG	55	1.9019659E-5	23.545454	21
ATTGGAC	240	0.0	22.354168	3
CGGGATA	50	2.7013663E-4	22.2	24
CGTCATT	60	3.724939E-5	21.583334	26
TATACCG	70	5.0995586E-6	21.142859	5
TCGGGAT	55	5.141705E-4	20.181818	23
CGTCTGT	350	0.0	20.085714	34
TGTGCGC	85	1.2445926E-6	19.588236	10
AGTATCA	125	4.110916E-10	19.240002	1
AGCCGTC	60	9.234342E-4	18.5	12
ACTGCGT	60	9.234342E-4	18.5	8
GCGCCAT	50	0.007033533	18.5	11
GCGCCAA	80	1.6159309E-5	18.5	13
AAGCCGT	70	1.2187629E-4	18.5	11
TAAGCCG	60	9.234342E-4	18.5	10
ATTGATC	50	0.007033533	18.5	8
>>END_MODULE