FastQCFastQC Report
Wed 25 May 2016
SRR2088185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052745
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140871.3381208174819164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139791.32786192287781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119241.1326579561052297No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG62970.5981505492783152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.41833492441189457No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT41040.3898379949560435No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36960.35108217089608595No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT36250.3443378975915345No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32700.31061653106877735No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA31340.2976979230487915No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT30360.2883889260932135No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT27770.2637865769963286No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG26080.24773330673619917No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23160.21999629539917073No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA22880.21733658198329128No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22670.21534179692138172No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19000.1804805532203905No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.17079159720540113No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17380.16509221131423088No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC16190.15378842929674327No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15750.14960887964321842No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.13165581408603222No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13700.13013597784838682No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13250.12586143843000916No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12740.12101696042251449No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC12650.12016205253883892No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12000.11398771782340453No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11660.11075806581840808No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11260.10695847522429458No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA11000.10448874133812082No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC10640.10106910980341868No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.10087913027371301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG200.00184186637.04
GGTATCA32150.030.4976671
TGCGGTC451.3231089E-424.66666612
AGCCGTC701.922599E-723.78571512
CAATGCG400.001931080223.12519
ATTGGAC2400.020.8124983
CGATCTG1251.8189894E-1120.7233
GAGCGAT909.476389E-820.55555521
ACGTTTA555.142699E-420.18181826
CGTCTGT2950.020.06779934
TTGCGTG656.901423E-519.9230797
ACGATCT1403.6379788E-1219.8214332
GTATTGG2550.018.8627451
GTATTAC609.236114E-418.51
ATAGGAC609.236114E-418.53
ACTGGGT701.2190735E-418.58
TTGGACC5150.018.3203894
GGATAAG1156.4033884E-817.6956521
CGGGATA1156.4033884E-817.69565224
GCCGTCA953.6058045E-617.52631613
Produced by FastQC (version 0.11.5)