##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1052745
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.502340072857148	31.0	31.0	33.0	28.0	34.0
2	30.935138138865536	31.0	31.0	34.0	28.0	34.0
3	31.13221245410807	31.0	31.0	34.0	28.0	34.0
4	35.17658787265672	35.0	35.0	37.0	33.0	37.0
5	35.03439009446732	35.0	35.0	37.0	32.0	37.0
6	35.06779799476607	37.0	35.0	37.0	32.0	37.0
7	34.87215612517751	36.0	35.0	37.0	32.0	37.0
8	34.9883808519632	36.0	35.0	37.0	32.0	37.0
9	36.46910505393044	38.0	35.0	39.0	32.0	39.0
10	36.3351628362044	38.0	35.0	39.0	32.0	39.0
11	36.38247058879406	38.0	35.0	39.0	32.0	39.0
12	36.20466589724957	38.0	35.0	39.0	32.0	39.0
13	36.268507568309516	38.0	35.0	39.0	32.0	39.0
14	37.147876266332304	39.0	36.0	41.0	31.0	41.0
15	37.22890538544472	39.0	36.0	41.0	32.0	41.0
16	37.19813820060889	39.0	36.0	41.0	32.0	41.0
17	37.30845171432778	39.0	36.0	41.0	32.0	41.0
18	37.32210269343478	39.0	36.0	40.0	32.0	41.0
19	37.43236301288536	39.0	36.0	41.0	32.0	41.0
20	37.403649506765646	39.0	36.0	41.0	32.0	41.0
21	37.28573871165382	39.0	36.0	41.0	31.0	41.0
22	37.24565208098827	39.0	36.0	41.0	31.0	41.0
23	37.11619717975388	39.0	36.0	40.0	31.0	41.0
24	37.028314549107336	39.0	36.0	40.0	31.0	41.0
25	36.94291210122109	39.0	35.0	40.0	31.0	41.0
26	36.66605208288807	38.0	35.0	40.0	30.0	41.0
27	36.540657044203485	38.0	35.0	40.0	30.0	41.0
28	36.38951502975554	38.0	35.0	40.0	30.0	41.0
29	36.248560667588066	38.0	35.0	40.0	30.0	41.0
30	36.06490460652865	38.0	35.0	40.0	30.0	41.0
31	35.79433386052653	38.0	34.0	40.0	29.0	41.0
32	35.45504561883457	38.0	34.0	40.0	27.0	41.0
33	35.58403317042589	38.0	34.0	40.0	28.0	41.0
34	35.546881723494295	38.0	34.0	40.0	28.0	41.0
35	35.44564828139768	38.0	34.0	40.0	27.0	41.0
36	35.341376116723424	38.0	34.0	40.0	27.0	41.0
37	35.202392792176646	38.0	34.0	40.0	26.0	41.0
38	35.05520045215128	38.0	34.0	40.0	25.0	41.0
39	34.98718968031195	38.0	34.0	40.0	25.0	41.0
40	34.80039705721708	38.0	33.0	40.0	24.0	41.0
41	34.64573852167429	38.0	33.0	40.0	23.0	41.0
42	34.41884026996091	38.0	33.0	40.0	22.0	41.0
43	34.06096633087785	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	4.0
16	15.0
17	34.0
18	68.0
19	189.0
20	347.0
21	779.0
22	1514.0
23	2421.0
24	3971.0
25	6425.0
26	9647.0
27	14219.0
28	19963.0
29	26982.0
30	34602.0
31	42506.0
32	52569.0
33	63770.0
34	77256.0
35	93669.0
36	114984.0
37	147727.0
38	173628.0
39	165455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.537684814461244	24.25516150634769	14.38154538848439	21.825608290706676
2	17.25289600045595	23.36292264508499	36.75581456098105	22.628366793478
3	19.70201710765665	26.013327064008852	32.90084493395837	21.383810894376133
4	13.000679176818696	15.917339906625061	35.304750913089116	35.777230003467125
5	10.995825199834718	40.901642847983126	34.62956366451515	13.472968287667003
6	31.164052073389094	36.114633648224405	15.592664890358062	17.128649388028443
7	23.991374929351363	36.07160328474607	21.35094443573705	18.58607735016552
8	27.666908890567043	33.71870680934129	20.282024611848072	18.332359688243592
9	25.232891155977942	13.798688191347383	24.52056290934652	36.44785774332816
10	16.342134135046948	28.288236942469446	33.69248963424191	21.677139288241694
11	30.89352122308821	22.07856603450978	25.57827394098286	21.449638801419148
12	20.22902032306019	28.243211793929202	31.61430355879154	19.913464324219067
13	30.832347814522986	21.43956988634475	29.25114818878266	18.47693411034961
14	20.692190416482624	23.15736479394345	29.097264769721065	27.053180019852864
15	22.731525678108184	29.54105695111352	27.314401873198165	20.413015497580137
16	21.363198115403065	28.704102132995168	27.74100090715225	22.191698844449512
17	18.98484438301773	27.77063771378634	28.293366389771503	24.951151513424428
18	19.8443117754062	27.103904554284274	33.18058979144997	19.871193878859554
19	19.324432792366622	25.592902364770197	34.66698963186717	20.415675210996014
20	23.853544780549896	24.28156866097678	33.22855962270065	18.636326935772672
21	23.139411728386268	25.293684605483758	31.796303948249577	19.7705997178804
22	21.237621646267616	25.135431657238932	32.610461222803245	21.01648547369021
23	20.36865527739386	27.101719789692662	33.57736203924027	18.952262893673208
24	18.8817804881524	26.34873592370422	32.08203316092691	22.68745042721647
25	20.050154595842297	27.30233817306185	33.47258832860759	19.17491890248826
26	21.38989023932671	26.128834618069902	31.97592959358629	20.505345549017093
27	20.10809835240253	27.698350502733334	31.34386769825551	20.849683446608626
28	19.717975388151928	27.227391248592962	31.557689658939246	21.49694370431586
29	18.772732238101344	27.235750347900012	32.498753259336304	21.492764154662336
30	18.14617974913203	27.28087048620511	34.91044839918499	19.66250136547787
31	19.85637547554251	26.615372193646138	33.38111318505431	20.147139145757045
32	20.937548979097503	26.961514896769874	31.421379346375428	20.679556777757195
33	21.87804264090544	26.86263055155807	30.757970828643217	20.501355978893272
34	19.909854713154658	29.723912248455232	30.87328840317456	19.492944635215554
35	18.450336976190815	30.435860536027242	31.0554787721623	20.058323715619643
36	19.572736037691936	26.64500900028022	33.39897126084664	20.3832837011812
37	19.49341958403982	26.932543018489756	32.45648281397679	21.11755458349363
38	20.032676479109377	26.592954609140868	32.945870082498615	20.428498829251147
39	21.039188027490038	26.02254107119958	32.77213380258277	20.166137098727614
40	19.116975145928023	26.783694056965363	32.117274363687315	21.9820564334193
41	20.014628423787336	26.130069485012992	32.090867209058224	21.764434882141448
42	17.47317726514968	27.8563184816836	32.5219307619604	22.14857349120632
43	18.06581840806653	27.594336710219476	32.23667649810733	22.10316838360667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	138.0
2	200.0
3	517.5
4	835.0
5	835.0
6	1440.0
7	2045.0
8	2309.5
9	2574.0
10	3889.5
11	5205.0
12	5205.0
13	9261.5
14	13318.0
15	22026.5
16	30735.0
17	30081.0
18	29427.0
19	29427.0
20	31190.5
21	32954.0
22	26941.0
23	20928.0
24	22933.5
25	24939.0
26	24939.0
27	26970.0
28	29001.0
29	29551.0
30	30101.0
31	32272.0
32	34443.0
33	34443.0
34	37318.0
35	40193.0
36	43225.5
37	46258.0
38	47551.0
39	48844.0
40	48844.0
41	51405.0
42	53966.0
43	61878.5
44	69791.0
45	88634.5
46	107478.0
47	107478.0
48	108870.5
49	110263.0
50	98802.0
51	87341.0
52	79692.5
53	72044.0
54	72044.0
55	62231.0
56	52418.0
57	42475.5
58	32533.0
59	28005.0
60	23477.0
61	23477.0
62	20180.5
63	16884.0
64	14509.0
65	12134.0
66	10199.5
67	8265.0
68	8265.0
69	6929.5
70	5594.0
71	4551.0
72	3508.0
73	2814.5
74	2121.0
75	2121.0
76	1736.5
77	1352.0
78	1041.5
79	731.0
80	573.0
81	415.0
82	415.0
83	289.5
84	164.0
85	128.5
86	93.0
87	77.5
88	62.0
89	62.0
90	46.5
91	31.0
92	16.0
93	1.0
94	2.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1052745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.49848515135764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93141875499894	37.01624336558552
2	12.197142565643238	11.586915900863952
3	4.139285389398379	5.898293568167116
4	1.8658240266778168	3.5449525930481043
5	0.9759214946119378	2.317739631035794
6	0.5810384816301385	1.6559068615245913
7	0.3801719080032056	1.2640312809077494
8	0.27907904342860523	1.060466544027896
9	0.21039869027809455	0.8994257159435254
>10	1.2460010678486777	11.245501011779739
>50	0.11038247975828773	3.626438429694788
>100	0.06759707692664581	6.4797903651148445
>500	0.009080204305415254	3.166958234466885
>1k	0.0058516872190453855	5.801026362598514
>5k	2.0178231789811673E-4	0.6035267984879694
>10k+	6.053469536943503E-4	3.8327833367530415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14087	1.3381208174819164	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13979	1.32786192287781	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11924	1.1326579561052297	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6297	0.5981505492783152	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4404	0.41833492441189457	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4104	0.3898379949560435	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3696	0.35108217089608595	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3625	0.3443378975915345	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3270	0.31061653106877735	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3134	0.2976979230487915	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3036	0.2883889260932135	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2777	0.2637865769963286	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2608	0.24773330673619917	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2316	0.21999629539917073	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2288	0.21733658198329128	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2267	0.21534179692138172	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1900	0.1804805532203905	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.17079159720540113	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1738	0.16509221131423088	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1619	0.15378842929674327	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1575	0.14960887964321842	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.13165581408603222	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1370	0.13013597784838682	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1325	0.12586143843000916	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1274	0.12101696042251449	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1265	0.12016205253883892	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1200	0.11398771782340453	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1166	0.11075806581840808	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1126	0.10695847522429458	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1100	0.10448874133812082	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1064	0.10106910980341868	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1062	0.10087913027371301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.49897648528371E-5	0.0
11	0.0	0.0	0.0	2.849692945585113E-4	0.0
12	0.0	0.0	0.0	3.799590594113484E-4	0.0
13	0.0	0.0	0.0	6.649283539698598E-4	0.0
14	0.0	0.0	0.0	7.599181188226969E-4	0.0
15	0.0	0.0	0.0	0.0015198362376453937	0.0
16	9.49897648528371E-5	0.0	0.0	0.0022797543564680904	0.0
17	9.49897648528371E-5	0.0	0.0	0.0039895701238191585	0.0
18	9.49897648528371E-5	0.0	0.0	0.004369529183230507	0.0
19	9.49897648528371E-5	0.0	0.0	0.005129447302053204	0.0
20	9.49897648528371E-5	0.0	0.0	0.005984355185728737	0.0
21	9.49897648528371E-5	0.0	0.0	0.008739058366461014	0.0
22	9.49897648528371E-5	0.0	0.0	0.014153474963072728	0.0
23	9.49897648528371E-5	0.0	0.0	0.023842430978062115	0.0
24	9.49897648528371E-5	0.0	0.0	0.03837586500054619	0.0
25	9.49897648528371E-5	0.0	0.0	0.044265230421422094	0.0
26	9.49897648528371E-5	0.0	0.0	0.055284043144351196	0.0
27	9.49897648528371E-5	0.0	0.0	0.14162973939558013	0.0
28	9.49897648528371E-5	0.0	0.0	0.23130007741665837	0.0
29	9.49897648528371E-5	9.49897648528371E-5	0.0	0.33645374710874904	0.0
30	9.49897648528371E-5	9.49897648528371E-5	0.0	0.5394468745992619	0.0
31	9.49897648528371E-5	9.49897648528371E-5	0.0	0.8344850842321739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	20	0.001841866	37.0	4
GGTATCA	3215	0.0	30.497667	1
TGCGGTC	45	1.3231089E-4	24.666666	12
AGCCGTC	70	1.922599E-7	23.785715	12
CAATGCG	40	0.0019310802	23.125	19
ATTGGAC	240	0.0	20.812498	3
CGATCTG	125	1.8189894E-11	20.72	33
GAGCGAT	90	9.476389E-8	20.555555	21
ACGTTTA	55	5.142699E-4	20.181818	26
CGTCTGT	295	0.0	20.067799	34
TTGCGTG	65	6.901423E-5	19.923079	7
ACGATCT	140	3.6379788E-12	19.82143	32
GTATTGG	255	0.0	18.862745	1
GTATTAC	60	9.236114E-4	18.5	1
ATAGGAC	60	9.236114E-4	18.5	3
ACTGGGT	70	1.2190735E-4	18.5	8
TTGGACC	515	0.0	18.320389	4
GGATAAG	115	6.4033884E-8	17.695652	1
CGGGATA	115	6.4033884E-8	17.695652	24
GCCGTCA	95	3.6058045E-6	17.526316	13
>>END_MODULE