FastQCFastQC Report
Wed 25 May 2016
SRR2088184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193749
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201381.6869542927365804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191561.604692443721419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165511.386472365631301No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78150.6546602342703534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.5276653634893097No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT50530.4232883127022515No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48190.4036862020407975No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT45350.3798956061952722No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41060.34395840331593996No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41040.3437908639085771No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT38850.3254452988023445No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35970.30131962414209357No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG31760.2660525788922127No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31000.2596860814124242No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT29900.2504714140074672No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28550.23916250401047456No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.20523577401949655No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23500.19685880365135386No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22190.1858849724690869No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21710.1818640266923784No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA21710.1818640266923784No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.15639803677322453No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16420.1375498534449034No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC16220.13587445937127487No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16180.13553938055654915No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15750.1319372832982478No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC15200.1273299495957693No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC15070.12624094344791073No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14940.1251519373000522No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14910.12490062818900789No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.12263884618960938No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC14110.11819905189449373No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA13550.11350794848833382No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12900.10806291774904105No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG12730.10663883278645679No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA12720.10655506308277535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12090.10127757175084544No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT12030.10077495352875689No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC11990.10043987471403118No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT11960.1001885656029869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG352.3883851E-531.7142854
GGTATCA45250.029.8453031
ATTGGAC2800.026.428573
GTATTGG3100.022.0806451
ACGCATT603.7264417E-521.58333217
TATTGGA3200.020.81252
CGTTTAT555.143253E-420.18181827
TTGGACC6400.019.0781254
TTTTTAC9150.019.0054631
GGACCCT6250.018.6486
ACGTTTA609.2371006E-418.526
ATCACGT801.6167562E-518.523
CGTTAGA701.21924735E-418.52
CTAGGAC609.2371006E-418.53
TGCGTTC1306.9667294E-1018.532
TCACGTT701.21924735E-418.524
TGGACCC6550.018.0763365
GTATCAA78100.017.5524962
CGTCTGT4850.017.54639234
GACCCTC6500.017.0769237
Produced by FastQC (version 0.11.5)