FastQCFastQC Report
Wed 25 May 2016
SRR2088183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501221
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192761.2840214731874922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT187601.2496494520127284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT164711.097173567382817No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95460.6358823917331292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.4295836522404096No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT55060.3667681174190875No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT51720.3445195610772831No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA47710.31780797097829033No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46610.31048060212320505No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA42370.28223692580905807No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT40410.26918088675817886No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT38880.25898918280519656No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37810.2518616512825227No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA31860.21222724702092496No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA30760.20489987816583966No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28530.19004530312325768No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25730.17139381876485874No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.16399983746563632No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC21740.14481545355414024No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA21140.14081870690591192No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19690.13115990250602674No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG18660.12429882075990144No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17320.1153727532455248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.11424034169519345No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16850.11224196837107928No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16210.10797877194630237No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.10458153729530828No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA15420.10271638885946839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.028.9258481
GTATTGG4700.023.617021
ATTGGAC4600.023.3260883
TGCGACG555.144101E-420.18181822
TATTGGA5750.018.9826072
TACGTTA902.1531087E-618.519
TTGGACC8550.017.9590644
GTATCAA78100.017.6946242
AGGTCGT1054.7996764E-717.61904722
GGACCCT8200.017.1463416
CTAGTAC1554.0199666E-1016.7096773
GGTCGTC1151.242739E-616.08695623
TGGACCC9000.016.0333335
CTTAACG1059.346832E-615.85714230
TAGACTC700.002592989515.8571425
GAACCGC700.002592989515.8571426
TACACGG700.002592989515.85714215
GACCCTC8150.015.6625777
CATTGCG957.061973E-515.57894829
AGACGCG957.061973E-515.57894814
Produced by FastQC (version 0.11.5)