##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501221
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.65061839662515	31.0	31.0	33.0	28.0	34.0
2	31.037879166358586	31.0	31.0	34.0	28.0	34.0
3	31.231746691526432	31.0	31.0	34.0	28.0	34.0
4	35.25622543249795	37.0	35.0	37.0	33.0	37.0
5	35.110620621480784	37.0	35.0	37.0	32.0	37.0
6	35.146172349041215	37.0	35.0	37.0	32.0	37.0
7	34.9773730849755	37.0	35.0	37.0	32.0	37.0
8	35.08350202934811	37.0	35.0	37.0	32.0	37.0
9	36.59525279755612	39.0	35.0	39.0	32.0	39.0
10	36.45519080801561	38.0	35.0	39.0	32.0	39.0
11	36.491180845458466	38.0	35.0	39.0	32.0	39.0
12	36.330308462245064	38.0	35.0	39.0	32.0	39.0
13	36.395488072708815	38.0	35.0	39.0	32.0	39.0
14	37.318885094199985	39.0	36.0	41.0	32.0	41.0
15	37.39736654363348	39.0	36.0	41.0	32.0	41.0
16	37.358320327253615	39.0	36.0	41.0	32.0	41.0
17	37.45212996620751	39.0	36.0	41.0	32.0	41.0
18	37.45107549121682	39.0	36.0	41.0	32.0	41.0
19	37.55158500980202	39.0	36.0	41.0	32.0	41.0
20	37.516871932913276	39.0	36.0	41.0	32.0	41.0
21	37.40757756519526	39.0	36.0	41.0	32.0	41.0
22	37.35224260785054	39.0	36.0	41.0	32.0	41.0
23	37.23448446298047	39.0	36.0	41.0	31.0	41.0
24	37.162017451128115	39.0	36.0	40.0	31.0	41.0
25	37.06037085812149	39.0	36.0	40.0	31.0	41.0
26	36.8080475826011	39.0	35.0	40.0	31.0	41.0
27	36.671246272201095	38.0	35.0	40.0	30.0	41.0
28	36.52278378733045	38.0	35.0	40.0	30.0	41.0
29	36.38783097225525	38.0	35.0	40.0	30.0	41.0
30	36.22273003108803	38.0	35.0	40.0	30.0	41.0
31	35.96002853677107	38.0	35.0	40.0	30.0	41.0
32	35.65028067153337	38.0	34.0	40.0	29.0	41.0
33	35.791858094178004	38.0	34.0	40.0	29.0	41.0
34	35.77433702299661	38.0	34.0	40.0	29.0	41.0
35	35.66998130188693	38.0	34.0	40.0	28.0	41.0
36	35.56684125788275	38.0	34.0	40.0	27.0	41.0
37	35.42721091698024	38.0	34.0	40.0	27.0	41.0
38	35.288282671238946	38.0	34.0	40.0	26.0	41.0
39	35.22991018644157	38.0	34.0	40.0	25.0	41.0
40	35.052775707240976	38.0	34.0	40.0	25.0	41.0
41	34.907824364300794	38.0	34.0	40.0	24.0	41.0
42	34.69913490418799	38.0	33.0	40.0	23.0	41.0
43	34.356028193050854	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	14.0
17	36.0
18	82.0
19	225.0
20	496.0
21	933.0
22	1798.0
23	3095.0
24	5199.0
25	8604.0
26	12832.0
27	18699.0
28	26336.0
29	36524.0
30	46466.0
31	57836.0
32	70995.0
33	87507.0
34	106697.0
35	129563.0
36	161600.0
37	209882.0
38	255998.0
39	259799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37147095597517	23.663537880165546	13.83720318327548	22.127787980583804
2	17.2798675211711	23.156017668284683	36.74961914335065	22.814495667193572
3	19.704893549983645	26.099754799593132	32.50853805002728	21.686813600395944
4	12.849940148718945	15.736657027845999	35.66057229415256	35.7528305292825
5	11.077316397785536	40.67549015101707	34.85229689699251	13.394896554204877
6	31.230445084367993	36.327962371962556	15.613757068412978	16.827835475256474
7	24.339321125936824	35.628331871190184	21.548526166367242	18.483820836505753
8	27.725564723648283	33.82153593641443	20.289417747287043	18.16348159265025
9	25.279688999820817	13.589071828864638	23.979214252931445	37.152024918383106
10	16.224060281597446	27.846133247536503	33.83552454968323	22.094281921182823
11	31.258355698461454	21.84128785834997	25.427568625805264	21.472787817383317
12	20.131479642237885	27.543046626712524	31.882114625361623	20.443359105687968
13	30.93501889461978	21.01309534039292	29.05228477352768	18.99960099145962
14	20.752174396707744	22.515405793017816	29.030569116738974	27.701850693535462
15	22.976230681558548	28.90027517600673	27.27706313727293	20.846431005161797
16	21.547593592149326	28.407409701836038	27.63363955073903	22.411357155275606
17	19.246067034767034	27.18960099812086	28.40574439073261	25.158587576379492
18	20.22680204979813	26.501960737293178	32.88802914427656	20.383208068632133
19	19.533566343662926	25.054538938637283	34.25058668910174	21.161308028598054
20	24.02710860026605	23.791367160464716	32.947314219558606	19.234210019710623
21	23.271656871306757	24.968608885700373	31.526604011001712	20.233130231991158
22	21.71179326694737	24.35797261029522	32.523858912178824	21.40637521057859
23	20.58491054947939	26.402308520864015	33.57220555800912	19.44057537164748
24	19.239938689906417	25.506171309887087	32.18886493061315	23.06502506959335
25	20.496848898330093	26.52121173364881	33.27937725358225	19.702562114438845
26	21.919624092655248	25.496645730375473	31.696265906219008	20.887464270750275
27	20.503110468078983	26.90696439764698	31.40556920000453	21.184355934269504
28	20.223737877367824	26.476714620965204	31.529268508767196	21.77027899289978
29	19.185050035937415	26.39571388889444	32.65395301557865	21.765283059589493
30	18.69804645685079	26.694137638628824	34.4983849812919	20.10943092322849
31	20.112828157879488	26.025615149268493	33.23867705021446	20.62287964263756
32	21.321444344303735	26.30372210354105	31.299921863603025	21.074911688552188
33	22.235833364974244	26.047064356280654	30.748570663479928	20.968531615265174
34	20.421376999122714	28.7818382503309	30.78394187131675	20.012842879229638
35	19.112375859383796	29.515108035392522	31.122732762198236	20.24978334302544
36	20.03535788534799	25.968061997534008	33.20477131614866	20.791808800969346
37	20.11456008142705	26.227184405227476	32.33427989616452	21.32397561718095
38	20.411651582278694	25.8891262512315	32.804563751772726	20.89465841471709
39	21.322510143409932	25.492848821059656	32.62690836325897	20.557732672271438
40	19.696566994466504	26.132861184329286	31.8747206440624	22.295851177141806
41	20.63453682036156	25.67763174109608	31.65723101395464	22.030600424587718
42	18.134971466559556	27.43160400767109	32.117256553165724	22.316167972603633
43	18.848590580600725	27.124920314863697	31.695000269780397	22.331488834755177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	133.0
2	197.0
3	641.5
4	1086.0
5	1086.0
6	1926.0
7	2766.0
8	3023.5
9	3281.0
10	5083.0
11	6885.0
12	6885.0
13	12236.5
14	17588.0
15	29160.0
16	40732.0
17	40058.5
18	39385.0
19	39385.0
20	41771.0
21	44157.0
22	36419.5
23	28682.0
24	30993.5
25	33305.0
26	33305.0
27	36135.5
28	38966.0
29	40520.0
30	42074.0
31	45319.0
32	48564.0
33	48564.0
34	51980.0
35	55396.0
36	60171.5
37	64947.0
38	67708.5
39	70470.0
40	70470.0
41	74194.5
42	77919.0
43	88521.5
44	99124.0
45	125743.5
46	152363.0
47	152363.0
48	153081.5
49	153800.0
50	137985.0
51	122170.0
52	113281.0
53	104392.0
54	104392.0
55	90448.5
56	76505.0
57	63082.5
58	49660.0
59	42978.5
60	36297.0
61	36297.0
62	31679.5
63	27062.0
64	23839.0
65	20616.0
66	17607.0
67	14598.0
68	14598.0
69	12488.5
70	10379.0
71	8811.5
72	7244.0
73	5988.0
74	4732.0
75	4732.0
76	3657.0
77	2582.0
78	2017.5
79	1453.0
80	1173.5
81	894.0
82	894.0
83	660.5
84	427.0
85	318.5
86	210.0
87	180.0
88	150.0
89	150.0
90	112.0
91	74.0
92	43.0
93	12.0
94	10.0
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1501221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.86154701153774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.50825453169847	35.08786911976184
2	12.908299539062204	11.839891722994246
3	4.4321788206995585	6.097997320471641
4	1.9200963525472028	3.522343565561027
5	1.058556651104521	2.427352280950297
6	0.6442136504923718	1.7726780770518151
7	0.42603666153495895	1.3677090267136875
8	0.30670881701945774	1.1252912664472787
9	0.2178843866327852	0.8993263536575589
>10	1.3611591774162666	11.705306403591024
>50	0.12601421705623841	3.956405337118908
>100	0.07576689190580557	6.629562673625329
>500	0.0076354034373185	2.5319413429672193
>1k	0.006167056622449556	5.569611016094385
>5k	5.873387259475769E-4	1.7961773302738009
>10k+	4.405040444606826E-4	3.670537162720131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19276	1.2840214731874922	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18760	1.2496494520127284	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16471	1.097173567382817	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9546	0.6358823917331292	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6449	0.4295836522404096	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5506	0.3667681174190875	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5172	0.3445195610772831	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4771	0.31780797097829033	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4661	0.31048060212320505	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4237	0.28223692580905807	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4041	0.26918088675817886	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3888	0.25898918280519656	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3781	0.2518616512825227	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3186	0.21222724702092496	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3076	0.20489987816583966	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2853	0.19004530312325768	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2573	0.17139381876485874	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2462	0.16399983746563632	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2174	0.14481545355414024	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2114	0.14081870690591192	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1969	0.13115990250602674	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1866	0.12429882075990144	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1732	0.1153727532455248	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1715	0.11424034169519345	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1685	0.11224196837107928	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1621	0.10797877194630237	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.10458153729530828	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1542	0.10271638885946839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.661244413713903E-5	0.0
3	0.0	0.0	0.0	1.3322488827427806E-4	0.0
4	0.0	6.661244413713903E-5	0.0	1.3322488827427806E-4	0.0
5	0.0	6.661244413713903E-5	0.0	1.3322488827427806E-4	0.0
6	0.0	6.661244413713903E-5	0.0	1.3322488827427806E-4	0.0
7	0.0	6.661244413713903E-5	0.0	2.664497765485561E-4	0.0
8	6.661244413713903E-5	6.661244413713903E-5	0.0	3.3306222068569516E-4	0.0
9	6.661244413713903E-5	6.661244413713903E-5	0.0	5.328995530971123E-4	0.0
10	6.661244413713903E-5	6.661244413713903E-5	0.0	9.325742179199464E-4	0.0
11	6.661244413713903E-5	6.661244413713903E-5	0.0	0.0011324115503313636	0.0
12	6.661244413713903E-5	6.661244413713903E-5	0.0	0.0015320862151541978	0.0
13	6.661244413713903E-5	6.661244413713903E-5	0.0	0.0018651484358398929	0.0
14	6.661244413713903E-5	6.661244413713903E-5	0.0	0.002398047988937005	0.0
15	6.661244413713903E-5	6.661244413713903E-5	0.0	0.0032640097627198127	0.0
16	6.661244413713903E-5	6.661244413713903E-5	0.0	0.004329808868914037	0.0
17	6.661244413713903E-5	6.661244413713903E-5	0.0	0.005861895084068235	0.0
18	6.661244413713903E-5	6.661244413713903E-5	0.0	0.006661244413713904	0.0
19	6.661244413713903E-5	6.661244413713903E-5	0.0	0.007527206187496711	0.0
20	6.661244413713903E-5	6.661244413713903E-5	0.0	0.010258316397119412	0.0
21	6.661244413713903E-5	6.661244413713903E-5	0.0	0.013855388380524919	0.0
22	6.661244413713903E-5	6.661244413713903E-5	0.0	0.02271484345076441	0.0
23	6.661244413713903E-5	6.661244413713903E-5	0.0	0.040300528702969116	0.0
24	6.661244413713903E-5	6.661244413713903E-5	0.0	0.06321520948614494	0.0
25	6.661244413713903E-5	6.661244413713903E-5	0.0	0.07127531522673877	0.0
26	6.661244413713903E-5	6.661244413713903E-5	0.0	0.0832655551714238	0.0
27	6.661244413713903E-5	6.661244413713903E-5	0.0	0.16546531123665337	0.0
28	6.661244413713903E-5	6.661244413713903E-5	0.0	0.2511955268411513	0.0
29	6.661244413713903E-5	6.661244413713903E-5	0.0	0.35231321704132834	0.0
30	6.661244413713903E-5	6.661244413713903E-5	0.0	0.5511513627906883	0.0
31	6.661244413713903E-5	6.661244413713903E-5	0.0	0.8313899152756323	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4720	0.0	28.925848	1
GTATTGG	470	0.0	23.61702	1
ATTGGAC	460	0.0	23.326088	3
TGCGACG	55	5.144101E-4	20.181818	22
TATTGGA	575	0.0	18.982607	2
TACGTTA	90	2.1531087E-6	18.5	19
TTGGACC	855	0.0	17.959064	4
GTATCAA	7810	0.0	17.694624	2
AGGTCGT	105	4.7996764E-7	17.619047	22
GGACCCT	820	0.0	17.146341	6
CTAGTAC	155	4.0199666E-10	16.709677	3
GGTCGTC	115	1.242739E-6	16.086956	23
TGGACCC	900	0.0	16.033333	5
CTTAACG	105	9.346832E-6	15.857142	30
TAGACTC	70	0.0025929895	15.857142	5
GAACCGC	70	0.0025929895	15.857142	6
TACACGG	70	0.0025929895	15.857142	15
GACCCTC	815	0.0	15.662577	7
CATTGCG	95	7.061973E-5	15.578948	29
AGACGCG	95	7.061973E-5	15.578948	14
>>END_MODULE