FastQCFastQC Report
Wed 25 May 2016
SRR2088182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605119
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173101.0784247149276782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159460.9934465918103269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148270.9237321345021772No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG98180.611668044549968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.37561078025990596No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT53950.33611215118629834No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA51610.32153379282159145No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50910.3171727454475338No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT49660.3093851608510023No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41430.2581117038674391No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT39990.24914040641223484No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT39870.24839279829096786No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG37770.23530965616879496No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA34360.2140651253894571No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA31350.19531262168100932No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28410.1769962227099673No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27730.1727597766894542No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23350.1454720802632079No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC21350.13301194490875753No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.13245123881780727No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19570.12192242444329672No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC19280.12011570481690144No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18970.11818438383696163No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18310.114072539169993No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG18160.11313802901840922No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17110.1065964579573228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.029.4318181
ATTGGAC5900.025.0847453
GTATTGG6200.024.4677411
TTGGACC10050.021.7213944
TATTGGA7400.020.752
TTGCGCG555.1443145E-420.18181818
TGGACCC10750.019.6186055
GACCCTC10900.019.3486237
GGACCCT10900.019.17896
TAGGTCG801.6173215E-518.521
TACGTAG500.007036066618.514
ACGTTTA1156.409573E-817.69565226
TGCGTTA953.608191E-617.52631637
CGTTTAG1201.0419353E-716.95833426
GTATCAA70800.016.7231642
GTTATCA1554.0199666E-1016.7096771
CGTCTGT5100.016.68627534
AGGTCGT904.447724E-516.44444522
CATTGCG803.3836107E-416.187529
ACCCTCG12400.016.1129028
Produced by FastQC (version 0.11.5)