##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1605119
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62006492976533	31.0	31.0	33.0	28.0	34.0
2	31.009186234790068	31.0	31.0	34.0	28.0	34.0
3	31.209252398108802	31.0	31.0	34.0	28.0	34.0
4	35.23000101550103	37.0	35.0	37.0	33.0	37.0
5	35.07497824148864	35.0	35.0	37.0	32.0	37.0
6	35.109265418950244	37.0	35.0	37.0	32.0	37.0
7	34.9299217067395	36.0	35.0	37.0	32.0	37.0
8	35.03037718698738	37.0	35.0	37.0	32.0	37.0
9	36.53850898282308	38.0	35.0	39.0	32.0	39.0
10	36.400287455322626	38.0	35.0	39.0	32.0	39.0
11	36.434750320692736	38.0	35.0	39.0	32.0	39.0
12	36.26676838290494	38.0	35.0	39.0	32.0	39.0
13	36.332843857682825	38.0	35.0	39.0	32.0	39.0
14	37.25539539436017	39.0	36.0	41.0	32.0	41.0
15	37.33373101932006	39.0	36.0	41.0	32.0	41.0
16	37.30696353354487	39.0	36.0	41.0	32.0	41.0
17	37.37231881249926	39.0	36.0	41.0	32.0	41.0
18	37.3697663537719	39.0	36.0	41.0	32.0	41.0
19	37.46772482289475	39.0	36.0	41.0	32.0	41.0
20	37.432969144343815	39.0	36.0	41.0	32.0	41.0
21	37.30547392436324	39.0	36.0	41.0	31.0	41.0
22	37.25461975093435	39.0	36.0	40.0	31.0	41.0
23	37.13996470043654	39.0	36.0	40.0	31.0	41.0
24	37.05924794361041	39.0	36.0	40.0	31.0	41.0
25	36.967083437427384	39.0	36.0	40.0	31.0	41.0
26	36.723817361828004	39.0	35.0	40.0	30.0	41.0
27	36.585501760305625	38.0	35.0	40.0	30.0	41.0
28	36.43981225068048	38.0	35.0	40.0	30.0	41.0
29	36.32058806854819	38.0	35.0	40.0	30.0	41.0
30	36.15017951940012	38.0	35.0	40.0	30.0	41.0
31	35.906722803729814	38.0	34.0	40.0	29.0	41.0
32	35.60356584153573	38.0	34.0	40.0	28.0	41.0
33	35.77581537568243	38.0	34.0	40.0	29.0	41.0
34	35.761962197195345	38.0	34.0	40.0	29.0	41.0
35	35.68530993652184	38.0	34.0	40.0	28.0	41.0
36	35.601594025115894	38.0	34.0	40.0	27.0	41.0
37	35.47761131729174	38.0	34.0	40.0	27.0	41.0
38	35.35741960564918	38.0	34.0	40.0	27.0	41.0
39	35.31444086077107	38.0	34.0	40.0	26.0	41.0
40	35.15970965392597	38.0	34.0	40.0	25.0	41.0
41	35.04415124361496	38.0	34.0	40.0	25.0	41.0
42	34.84733966765081	38.0	33.0	40.0	24.0	41.0
43	34.52209275449359	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	4.0
16	14.0
17	42.0
18	95.0
19	241.0
20	542.0
21	1029.0
22	1904.0
23	3429.0
24	5592.0
25	8936.0
26	13742.0
27	20102.0
28	28683.0
29	39005.0
30	50193.0
31	62827.0
32	77253.0
33	94848.0
34	115341.0
35	139915.0
36	173182.0
37	223522.0
38	274444.0
39	270231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.24879152262231	23.38804786436395	13.544665535701714	22.818495077312026
2	17.467614550696865	22.80640874601821	36.569687356513754	23.156289346771175
3	19.82687887938527	26.037072640720094	31.885984777452634	22.250063702442
4	13.106754078669555	15.674476471837915	35.68613915852968	35.53263029096285
5	11.523631581209868	39.9973459911695	34.90644618872495	13.572576238895683
6	31.877823388795473	36.08424048310437	15.612051193712118	16.42588493438804
7	25.013161017968137	34.71649142524635	21.697145196088265	18.573202360697245
8	27.49416086906952	33.84110461591944	20.316562198815166	18.34817231619587
9	25.432070768584758	13.74153567430203	23.494145916907094	37.33224764020612
10	16.214249535392703	27.46730927738068	34.138528046830174	22.179913140396444
11	31.657465895052017	21.72636421349445	25.17532967960631	21.44084021184722
12	20.236069724425416	26.931648058492858	31.975697752004677	20.856584465077045
13	30.834847758951206	20.901129448969204	28.655383183427524	19.60863960865207
14	21.079371685214614	22.35965059288439	28.208562729616933	28.352414992284057
15	23.09585769030209	28.724723836675036	26.73322040297324	21.446198070049636
16	22.11306451421982	28.328803035787374	26.95463700822182	22.603495441770985
17	19.661096778494304	27.000739509033288	27.892012990937122	25.44615072153529
18	20.861381617188506	26.552486139656935	31.818014739094107	20.768117504060445
19	20.131840692185442	25.03266112979785	33.08602041343975	21.74947776457696
20	24.36903432081983	24.005696773884054	31.816332620821257	19.80893628447486
21	23.669896126081618	25.004438923220025	30.542408382182256	20.783256568516105
22	22.099669868713782	24.518618245750005	31.450876850875233	21.93083503466098
23	21.102298334266806	26.36190836941062	32.527494846176516	20.008298450146064
24	20.02779856197578	25.584645126000005	30.933158226897817	23.4543980851264
25	21.043050390656393	26.441466333648783	32.09593805817513	20.419545217519698
26	22.445563226153325	25.56065936544269	30.67199378986854	21.32178361853545
27	21.03488900199923	26.9153875818553	30.23545294772537	21.814270468420098
28	20.881193232402083	26.33001042290322	30.51755041215013	22.271245932544563
29	20.04250152169403	26.366020214077583	31.376489842809164	22.21498842141922
30	19.42883985548735	26.480964962722393	33.28606788655545	20.80412729523481
31	20.803940393204492	25.931286091560814	32.18733315100002	21.077440364234675
32	21.80093812359084	26.092894047108032	30.245732559392792	21.860435269908336
33	22.67501661870553	25.94735966616805	29.833239778483712	21.544383936642703
34	20.952963612043717	28.56847373933023	29.908312093994276	20.570250554631777
35	19.68732536341542	29.428783784878256	30.070356154278905	20.813534697427418
36	20.5980366564722	26.022868086416022	32.113008443610724	21.266086813501055
37	20.640151913970243	26.232945968492054	31.315435179572354	21.811466937965346
38	20.880134120896958	25.962934835361118	31.86623546291583	21.290695580826092
39	21.81620178940004	25.355939341569066	31.794091279213564	21.03376758981733
40	20.29569147209646	26.083548945592195	30.996642616528742	22.624116965782598
41	21.119617922409493	25.652428262328215	30.843382951669003	22.384570863593293
42	18.806580695886097	27.285142098498618	31.239179151203118	22.669098054412164
43	19.435319125871665	27.008028688215642	30.90680504062316	22.649847145289538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	152.0
2	212.0
3	637.0
4	1062.0
5	1062.0
6	1791.0
7	2520.0
8	2768.5
9	3017.0
10	4656.5
11	6296.0
12	6296.0
13	11455.5
14	16615.0
15	26499.5
16	36384.0
17	36306.0
18	36228.0
19	36228.0
20	38480.5
21	40733.0
22	33773.5
23	26814.0
24	29455.0
25	32096.0
26	32096.0
27	35434.0
28	38772.0
29	41066.0
30	43360.0
31	46877.0
32	50394.0
33	50394.0
34	55178.5
35	59963.0
36	65466.5
37	70970.0
38	74300.0
39	77630.0
40	77630.0
41	81517.5
42	85405.0
43	96117.0
44	106829.0
45	133223.0
46	159617.0
47	159617.0
48	161291.5
49	162966.0
50	148602.0
51	134238.0
52	125095.0
53	115952.0
54	115952.0
55	101869.0
56	87786.0
57	72364.0
58	56942.0
59	50339.5
60	43737.0
61	43737.0
62	38760.0
63	33783.0
64	29498.0
65	25213.0
66	21357.0
67	17501.0
68	17501.0
69	14757.5
70	12014.0
71	10119.0
72	8224.0
73	6644.5
74	5065.0
75	5065.0
76	3992.5
77	2920.0
78	2345.5
79	1771.0
80	1380.5
81	990.0
82	990.0
83	705.5
84	421.0
85	342.0
86	263.0
87	230.5
88	198.0
89	198.0
90	149.0
91	100.0
92	57.5
93	15.0
94	11.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1605119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.24380201238885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87254520806617	39.62856545647266
2	12.089580258443524	12.148529538362427
3	3.8889828791464	5.861918574282051
4	1.6454265285522507	3.306899389060463
5	0.8740830327868231	2.1958627420864767
6	0.5017188873694199	1.5124958665719093
7	0.33519710019107196	1.1789103715988962
8	0.25136993151934267	1.0103824856900478
9	0.1822333336378073	0.8240485981820034
>10	1.1954704622112713	11.451150831447714
>50	0.09619705493492231	3.301452678398796
>100	0.05478435766350563	5.246432732679571
>500	0.006017437807276172	2.1808086662054254
>1k	0.005390621369018237	5.140203125755312
>5k	6.268164382579346E-4	1.9837214606901095
>10k+	3.7608986295476073E-4	3.028617482516115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17310	1.0784247149276782	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15946	0.9934465918103269	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14827	0.9237321345021772	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9818	0.611668044549968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6029	0.37561078025990596	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5395	0.33611215118629834	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5161	0.32153379282159145	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5091	0.3171727454475338	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4966	0.3093851608510023	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4143	0.2581117038674391	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3999	0.24914040641223484	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3987	0.24839279829096786	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3777	0.23530965616879496	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3436	0.2140651253894571	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3135	0.19531262168100932	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2841	0.1769962227099673	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2773	0.1727597766894542	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2335	0.1454720802632079	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2135	0.13301194490875753	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2126	0.13245123881780727	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1957	0.12192242444329672	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1928	0.12011570481690144	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1897	0.11818438383696163	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1831	0.114072539169993	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1816	0.11313802901840922	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1711	0.1065964579573228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.230067677225178E-5	0.0	0.0	0.0
3	0.0	6.230067677225178E-5	0.0	0.0	0.0
4	0.0	6.230067677225178E-5	0.0	0.0	0.0
5	0.0	6.230067677225178E-5	0.0	0.0	0.0
6	0.0	6.230067677225178E-5	0.0	0.0	0.0
7	0.0	6.230067677225178E-5	0.0	0.0	0.0
8	6.230067677225178E-5	6.230067677225178E-5	0.0	0.0	0.0
9	6.230067677225178E-5	6.230067677225178E-5	0.0	1.8690203031675534E-4	0.0
10	6.230067677225178E-5	6.230067677225178E-5	0.0	5.60706090950266E-4	0.0
11	6.230067677225178E-5	6.230067677225178E-5	0.0	8.099087980392731E-4	0.0
12	6.230067677225178E-5	6.230067677225178E-5	0.0	0.001121412181900532	0.0
13	6.230067677225178E-5	6.230067677225178E-5	0.0	0.0014329155657617908	0.0
14	6.230067677225178E-5	6.230067677225178E-5	0.0	0.0019313209799398051	0.0
15	6.230067677225178E-5	6.230067677225178E-5	0.0	0.002305125040573316	0.0
16	6.230067677225178E-5	6.230067677225178E-5	0.0	0.0032396351921570926	0.0
17	6.230067677225178E-5	6.230067677225178E-5	0.0	0.004547949404374379	0.0
18	6.230067677225178E-5	6.230067677225178E-5	0.0	0.0048594527882356384	0.0
19	6.230067677225178E-5	6.230067677225178E-5	0.0	0.006043165646908422	0.0
20	6.230067677225178E-5	6.230067677225178E-5	0.0	0.007600682566214717	0.0
21	6.230067677225178E-5	6.230067677225178E-5	0.0	0.010591115051282803	0.0
22	6.230067677225178E-5	6.230067677225178E-5	0.0	0.015948973253696454	0.0
23	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.02554327747662323	0.0
24	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.04012163584133015	0.0
25	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.046476304872099826	0.0
26	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.05719202127692713	0.0
27	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.1315167286662235	0.0
28	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.2092679732779937	0.0
29	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.29991545798162006	0.0
30	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.47124231910531245	0.0
31	6.230067677225178E-5	1.2460135354450355E-4	0.0	0.7191367119821023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3960	0.0	29.431818	1
ATTGGAC	590	0.0	25.084745	3
GTATTGG	620	0.0	24.467741	1
TTGGACC	1005	0.0	21.721394	4
TATTGGA	740	0.0	20.75	2
TTGCGCG	55	5.1443145E-4	20.181818	18
TGGACCC	1075	0.0	19.618605	5
GACCCTC	1090	0.0	19.348623	7
GGACCCT	1090	0.0	19.1789	6
TAGGTCG	80	1.6173215E-5	18.5	21
TACGTAG	50	0.0070360666	18.5	14
ACGTTTA	115	6.409573E-8	17.695652	26
TGCGTTA	95	3.608191E-6	17.526316	37
CGTTTAG	120	1.0419353E-7	16.958334	26
GTATCAA	7080	0.0	16.723164	2
GTTATCA	155	4.0199666E-10	16.709677	1
CGTCTGT	510	0.0	16.686275	34
AGGTCGT	90	4.447724E-5	16.444445	22
CATTGCG	80	3.3836107E-4	16.1875	29
ACCCTCG	1240	0.0	16.112902	8
>>END_MODULE