FastQCFastQC Report
Wed 25 May 2016
SRR2088181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712540
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT224721.3122029266469688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT223591.3056045406238685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198111.156819694722459No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG142060.8295280694173566No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA75970.4436100762609924No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA73460.428953484298177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72390.4227054550550644No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT72220.4217127775117661No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT69300.40466208088570194No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA57950.338386256671377No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT55260.3226785943685987No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT54640.31905824097539326No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG54330.3172480642787906No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45580.26615436719726254No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA42960.25085545447113644No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39660.2315858315718173No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39070.22814065656860572No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC31640.18475480864680532No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30650.17897392177700958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.15748537260443551No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26810.15655108785780186No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25030.14615717005150244No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG24850.14510609971153957No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24730.14440538615156434No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24230.1414857463183342No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC20410.11917969799245566No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG19830.11579291578590865No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC19440.11351559671598911No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA18850.1100704217127775No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC18200.10627488992957829No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC18020.10522381958961542No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC17600.10277132212970208No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA17560.10253775094304367No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA17360.1013698950097516No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.10101953822976396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.030.2414881
CGCGTAT250.005497085429.624
ACGTTTA601.3380468E-624.66666826
GTATTGG6650.023.0902271
ATTGGAC7200.022.611113
TATACTG2150.021.5116275
TATTGGA7750.020.5290342
ACAGCGT555.144508E-420.1818188
TTGGACC12100.019.570254
GGACCCT12350.019.3238876
TGGACCC12300.019.2520335
AACGGTA609.2393346E-418.518
TATCGGC500.007036254318.52
CAAACGG650.001580361417.07692316
TACGTTA1005.881173E-616.65000219
GTATCAA89250.016.6448172
GACCCTC14100.016.400717
GTTATCA2400.016.18751
TACACTG3100.016.1129025
GATCTAT1151.2429882E-616.0869561
Produced by FastQC (version 0.11.5)