FastQCFastQC Report
Wed 25 May 2016
SRR2088180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096165
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152551.3916700496731789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144381.317137474741485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128941.176282767649031No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG84730.7729675733124118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.43889377967732957No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT45180.412164227100847No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT44440.40541341860030194No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41430.3779540488886254No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41070.3746698717802521No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA36250.3306983893848098No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT35050.31975113235689884No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34110.31117578101836857No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG31810.2901935383815393No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27020.24649573741179473No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26850.24494487599950737No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25370.23144325899841722No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21330.19458749367111702No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18700.1705947553516122No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18670.17032107392591442No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.15955627118180202No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16870.15390018838404804No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15840.14450379276842445No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15220.13884770997067047No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14190.12945131435504692No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14120.12881272436175212No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.116040924495856No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC11830.10792170886682205No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG11810.10773925458302354No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11770.1073743460154265No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA11490.10481998604224729No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA11450.10445507747465026No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11390.10390771462325471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.030.5558781
ATTGGAC3650.024.8356173
GTATTGG4050.022.8395061
GGACCCT5600.022.7946436
TTGGACC6250.021.9039974
TCTATAC603.726083E-521.5833343
GATCGAT705.101567E-621.14285926
TGGACCC6450.020.9379845
TGAACCG450.003825697620.5555555
CGCGAAT656.901761E-519.92307535
ATCGATG656.901761E-519.92307527
GACCCTC6400.019.656257
TATTGGA4950.018.6868692
CGACGAA902.1520318E-618.510
CGGATCG701.2191316E-418.524
GACGCGA701.2191316E-418.533
GAACCGT500.00703467918.56
CTGTGCG701.2191316E-418.59
GTATCAA59650.018.019282
CGTCTGT3550.017.7183134
Produced by FastQC (version 0.11.5)