FastQCFastQC Report
Wed 25 May 2016
SRR2088179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665541
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT275151.6520157714520387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT268221.6104076693398723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233911.4044085375262452No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG155860.935792033939723No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86370.5185702423416776No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA85210.5116055383806223No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT84200.5055414426903931No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT84140.5051811993820626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83830.503319942289022No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA68400.4106773714967089No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT66870.40149116713428246No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT64030.3844396505399747No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59570.3576615646207449No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51400.3086084341364157No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50690.3043455549878388No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47380.2844721324782758No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45510.2732445493686436No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38530.231336244499535No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36560.21950825587601866No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.19981495502062094No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30830.18510501993046102No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30810.18498493882768421No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG28610.1717760175222345No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28390.17045512539168955No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28030.16829366554170685No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22970.13791314653917255No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.13503120007252897No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC22370.1343107134558681No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG22340.13413059180170286No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC21820.13100848312950567No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA21770.13070828037256363No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA21530.12926730713924184No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA21390.12842673941980415No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC20620.12380361696289674No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.12362349530873151No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC20240.12152207601013724No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC20210.12134195435597203No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC19980.11996102167403866No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19590.11761944016989075No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT18800.11287623661020653No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG18720.11239591219909928No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG18190.10921376297551366No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT17890.10741254643386142No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG17850.1071723842283078No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT17320.1039902350047222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC17290.10381011335055697No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA17270.10369003224778015No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC17190.1032097078366729No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC16780.10074804522974816No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT16780.10074804522974816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58400.031.2345891
ATTGGAC5850.025.9316233
GTATTGG6000.024.3583341
ACGTTTA1005.347829E-1022.226
TTGGACC12150.021.4691354
TATACCG957.1504473E-921.4210535
CGGGCGT1251.8189894E-1120.726
GGACCCT11900.020.3655456
TCACGTT1202.382876E-1020.04166624
TGGTCGG656.904509E-519.92307737
ATTCCGC759.26987E-619.73333415
TATTGGA7850.019.324842
ACCGTCG1254.129106E-1019.2423
TGGACCC12700.019.2283465
GCGTCGG1457.2759576E-1219.137939
GACCCTC12850.018.5719837
CTACGCA609.2391914E-418.527
CGTCTGT6850.018.0948934
GACCGTC1351.1532393E-917.81481622
ATCACGT1258.58563E-917.7623
Produced by FastQC (version 0.11.5)