##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1665541
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54747436418557	31.0	31.0	33.0	28.0	34.0
2	30.952971436908488	31.0	31.0	34.0	28.0	34.0
3	31.1409674093883	31.0	31.0	34.0	28.0	34.0
4	35.18333682569207	35.0	35.0	37.0	33.0	37.0
5	35.06848105210259	35.0	35.0	37.0	32.0	37.0
6	35.1112371295573	37.0	35.0	37.0	32.0	37.0
7	34.90640338484612	36.0	35.0	37.0	32.0	37.0
8	35.03525881380284	37.0	35.0	37.0	32.0	37.0
9	36.493446874018716	38.0	35.0	39.0	32.0	39.0
10	36.39577830866968	38.0	35.0	39.0	32.0	39.0
11	36.444723366161504	38.0	35.0	39.0	32.0	39.0
12	36.25895189611063	38.0	35.0	39.0	32.0	39.0
13	36.30835566341507	38.0	35.0	39.0	32.0	39.0
14	37.16033829248274	39.0	36.0	41.0	31.0	41.0
15	37.23107807012857	39.0	36.0	41.0	32.0	41.0
16	37.1868882243067	39.0	36.0	41.0	32.0	41.0
17	37.35388081109981	39.0	36.0	41.0	32.0	41.0
18	37.376867936604384	39.0	36.0	41.0	32.0	41.0
19	37.4965035384899	39.0	36.0	41.0	32.0	41.0
20	37.46805092159244	39.0	36.0	41.0	32.0	41.0
21	37.35689724840157	39.0	36.0	41.0	31.0	41.0
22	37.31133907841356	39.0	36.0	41.0	31.0	41.0
23	37.17157608248611	39.0	36.0	41.0	31.0	41.0
24	37.0786609275905	39.0	36.0	40.0	31.0	41.0
25	36.99361348654882	39.0	35.0	40.0	31.0	41.0
26	36.714466350573176	38.0	35.0	40.0	30.0	41.0
27	36.58474753848749	38.0	35.0	40.0	30.0	41.0
28	36.42531045468109	38.0	35.0	40.0	30.0	41.0
29	36.2797511439226	38.0	35.0	40.0	30.0	41.0
30	36.079155661733935	38.0	35.0	40.0	30.0	41.0
31	35.76906902922234	38.0	34.0	40.0	29.0	41.0
32	35.45783742339576	38.0	34.0	40.0	28.0	41.0
33	35.54028811058989	38.0	34.0	40.0	29.0	41.0
34	35.469598767007234	38.0	34.0	40.0	28.0	41.0
35	35.331846529145785	38.0	34.0	40.0	27.0	41.0
36	35.218319452958525	38.0	34.0	40.0	26.0	41.0
37	35.055462459345044	38.0	34.0	40.0	25.0	41.0
38	34.89997664422551	38.0	33.0	40.0	25.0	41.0
39	34.798221118543466	38.0	33.0	40.0	24.0	41.0
40	34.574932109146516	38.0	33.0	40.0	23.0	41.0
41	34.397900742161255	38.0	33.0	40.0	22.0	41.0
42	34.12380241615187	38.0	33.0	40.0	21.0	41.0
43	33.75726505681938	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	6.0
16	13.0
17	55.0
18	129.0
19	286.0
20	622.0
21	1208.0
22	2237.0
23	3921.0
24	6494.0
25	10166.0
26	15704.0
27	23021.0
28	32042.0
29	43693.0
30	55732.0
31	67904.0
32	82150.0
33	101243.0
34	121853.0
35	146144.0
36	181090.0
37	236263.0
38	271933.0
39	261625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09580070379534	24.307177067391315	14.612969599667617	20.984052629145726
2	17.23157820792163	23.161002941386613	37.41781199021819	22.189606860473564
3	19.85913285833252	26.276687274585253	33.26780907825145	20.596370788830775
4	12.274630285294688	15.162941050385431	34.929491378477024	37.63293728584286
5	10.317188228929819	41.41699303709726	34.60407158995185	13.661747144021072
6	31.12003847398533	35.31327058295173	15.293649330757994	18.27304161230495
7	23.561293297493126	36.79008802545239	20.59474969394329	19.053868983111194
8	28.483657862520346	33.09195030323481	19.98906061153703	18.435331222707816
9	24.874380156357603	13.513386941540318	25.04639633608539	36.565836566016685
10	16.629131315290348	27.73086942921249	33.00405093600218	22.635948319494986
11	30.2226123523828	21.24901158242277	26.413219488442497	22.115156576751936
12	20.525402857089677	27.638647142279897	31.925362389758043	19.910587610872383
13	32.10140128642885	20.57877890727397	30.09664727556992	17.223172530727254
14	20.69123486002446	22.78857140112432	29.258180975430808	27.26201276342041
15	22.7120196981041	28.685033871877064	28.473511009335706	20.12943542068313
16	21.15426759233186	28.69854299593946	27.820870215743714	22.32631919598497
17	18.348692707054344	26.735697289949634	28.283362583088618	26.632247419907408
18	19.83937951692573	26.16885444429167	34.30741122554173	19.684354813240862
19	18.623978635170193	24.04089722198373	35.99869351760179	21.33643062524429
20	25.0603257440075	22.236378450005134	34.37807895452589	18.325216851461477
21	23.98584003636056	23.827152859040996	32.53927702770451	19.647730076893936
22	21.51529142783036	23.187660946203064	33.78193631979038	21.51511130617619
23	20.110462606444393	26.016591605970675	35.43413221289659	18.438813574688346
24	18.165448944216926	24.683871486802186	33.34718268718693	23.803496881793965
25	19.852288235474237	26.10467109485747	34.99283416019179	19.0502065094765
26	22.13887259455036	24.542475988282487	32.82272847080919	20.495922946357968
27	19.952676037395655	26.605589415090954	32.32006897458544	21.121665572927956
28	19.525547554818523	25.878678459431498	32.41175089655554	22.18402308919444
29	18.387298781597092	25.757276464524136	33.68827305962447	22.1671516942543
30	17.345715296110995	26.00080094095552	36.87684662220864	19.776637140724844
31	19.543980004094767	25.21354923115072	34.97242037271974	20.27005039203478
32	21.382962052570306	25.37800030140357	32.03265485508913	21.206382790937
33	22.67719617829882	25.350141485559348	31.195209244323618	20.777453091818213
34	19.777897992304002	29.649945573240167	31.310306981335195	19.26184945312064
35	17.93177111821324	30.817854378847475	31.52957507500566	19.720799427933628
36	19.344044967971367	25.490936578565165	34.78143137875321	20.38358707471026
37	19.42275813084157	26.191309610510938	33.37744312508668	21.008489133560808
38	20.067413531098904	25.40309725188392	34.151666035240204	20.37782318177697
39	21.478786772586204	24.819383011285822	33.84521906095377	19.856611155174207
40	18.922920540533074	26.03712547454551	32.5170620236908	22.522891961230616
41	20.454194763143025	25.46007573515152	32.20431079150858	21.88141871019687
42	17.03062248242463	27.729548537081943	32.945031073987366	22.29479790650605
43	17.96233175886994	27.671249161683804	32.29971522766476	22.066703851781494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	161.0
2	213.0
3	695.0
4	1177.0
5	1177.0
6	2209.5
7	3242.0
8	3618.5
9	3995.0
10	6287.5
11	8580.0
12	8580.0
13	15455.0
14	22330.0
15	38676.5
16	55023.0
17	53741.0
18	52459.0
19	52459.0
20	55085.0
21	57711.0
22	45052.0
23	32393.0
24	34196.5
25	36000.0
26	36000.0
27	37734.5
28	39469.0
29	39639.0
30	39809.0
31	42104.0
32	44399.0
33	44399.0
34	47756.0
35	51113.0
36	55769.5
37	60426.0
38	62726.5
39	65027.0
40	65027.0
41	69773.5
42	74520.0
43	91686.5
44	108853.0
45	151319.0
46	193785.0
47	193785.0
48	196655.0
49	199525.0
50	176047.0
51	152569.0
52	140210.5
53	127852.0
54	127852.0
55	106595.0
56	85338.0
57	65667.0
58	45996.0
59	38668.5
60	31341.0
61	31341.0
62	27001.0
63	22661.0
64	19842.5
65	17024.0
66	14345.5
67	11667.0
68	11667.0
69	9891.0
70	8115.0
71	6747.0
72	5379.0
73	4286.5
74	3194.0
75	3194.0
76	2488.5
77	1783.0
78	1437.5
79	1092.0
80	868.5
81	645.0
82	645.0
83	471.0
84	297.0
85	247.5
86	198.0
87	157.5
88	117.0
89	117.0
90	101.0
91	85.0
92	52.5
93	20.0
94	14.5
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1665541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.42834752925902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24466196820984	28.16283357867777
2	13.04624470065632	9.765987612158373
3	4.59027782518678	5.154195410908263
4	2.1193599486212045	3.17296562786348
5	1.1768242880692694	2.2023294217364726
6	0.7026664401916605	1.5779786232384458
7	0.4841693831557983	1.2685161955047533
8	0.3294309192456704	0.9864043945928174
9	0.25741958509933516	0.8671310722742024
>10	1.7108837624992908	12.441655496584614
>50	0.20038397910062924	5.214308807991127
>100	0.11432272167519635	8.43452401255994
>500	0.009799081799562556	2.530239838846578
>1k	0.01110562603950423	7.720187010528503
>5k	0.0017964983299198019	4.796715637767976
>10k+	6.532721199708371E-4	5.70402725876669
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27515	1.6520157714520387	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26822	1.6104076693398723	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23391	1.4044085375262452	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15586	0.935792033939723	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8637	0.5185702423416776	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8521	0.5116055383806223	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8420	0.5055414426903931	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8414	0.5051811993820626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8383	0.503319942289022	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6840	0.4106773714967089	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6687	0.40149116713428246	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6403	0.3844396505399747	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5957	0.3576615646207449	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5140	0.3086084341364157	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5069	0.3043455549878388	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4738	0.2844721324782758	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4551	0.2732445493686436	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3853	0.231336244499535	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3656	0.21950825587601866	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3328	0.19981495502062094	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3083	0.18510501993046102	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3081	0.18498493882768421	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2861	0.1717760175222345	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2839	0.17045512539168955	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2803	0.16829366554170685	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2297	0.13791314653917255	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2249	0.13503120007252897	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2237	0.1343107134558681	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2234	0.13413059180170286	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2182	0.13100848312950567	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2177	0.13070828037256363	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2153	0.12926730713924184	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2139	0.12842673941980415	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2062	0.12380361696289674	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2059	0.12362349530873151	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2024	0.12152207601013724	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2021	0.12134195435597203	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1998	0.11996102167403866	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1959	0.11761944016989075	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	1880	0.11287623661020653	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1872	0.11239591219909928	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	1819	0.10921376297551366	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	1789	0.10741254643386142	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1785	0.1071723842283078	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	1732	0.1039902350047222	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1729	0.10381011335055697	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1727	0.10369003224778015	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC	1719	0.1032097078366729	No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC	1678	0.10074804522974816	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	1678	0.10074804522974816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	6.004055138840773E-5	0.0
7	0.0	0.0	0.0	6.004055138840773E-5	0.0
8	0.0	0.0	0.0	6.004055138840773E-5	0.0
9	0.0	0.0	0.0	2.4016220555363093E-4	0.0
10	0.0	0.0	0.0	3.602433083304464E-4	0.0
11	0.0	0.0	0.0	5.403649624956695E-4	0.0
12	0.0	0.0	0.0	0.001080729924991339	0.0
13	0.0	0.0	0.0	0.0011407704763797468	0.0
14	0.0	0.0	0.0	0.0014409732333217856	0.0
15	0.0	0.0	0.0	0.0029419870180319788	0.0
16	0.0	0.0	0.0	0.004623122456907395	0.0
17	0.0	0.0	0.0	0.006724541755501666	0.0
18	0.0	0.0	0.0	0.00768519057771619	0.0
19	0.0	0.0	0.0	0.008886001605484344	0.0
20	0.0	0.0	0.0	0.011707907520739507	0.0
21	0.0	0.0	0.0	0.017351719351249833	0.0
22	0.0	0.0	0.0	0.031101005619195203	0.0
23	0.0	0.0	0.0	0.05223527970791472	0.0
24	0.0	0.0	0.0	0.08333628532710993	0.0
25	0.0	0.0	0.0	0.09348313851175084	0.0
26	0.0	0.0	0.0	0.10465068106999467	0.0
27	0.0	0.0	0.0	0.1957922380775976	0.0
28	0.0	0.0	0.0	0.29185712029905	0.0
29	0.0	0.0	0.0	0.40959664157171755	0.0
30	0.0	0.0	0.0	0.6589450514877748	0.0
31	0.0	0.0	0.0	0.9716362431186023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5840	0.0	31.234589	1
ATTGGAC	585	0.0	25.931623	3
GTATTGG	600	0.0	24.358334	1
ACGTTTA	100	5.347829E-10	22.2	26
TTGGACC	1215	0.0	21.469135	4
TATACCG	95	7.1504473E-9	21.421053	5
CGGGCGT	125	1.8189894E-11	20.72	6
GGACCCT	1190	0.0	20.365545	6
TCACGTT	120	2.382876E-10	20.041666	24
TGGTCGG	65	6.904509E-5	19.923077	37
ATTCCGC	75	9.26987E-6	19.733334	15
TATTGGA	785	0.0	19.32484	2
ACCGTCG	125	4.129106E-10	19.24	23
TGGACCC	1270	0.0	19.228346	5
GCGTCGG	145	7.2759576E-12	19.13793	9
GACCCTC	1285	0.0	18.571983	7
CTACGCA	60	9.2391914E-4	18.5	27
CGTCTGT	685	0.0	18.09489	34
GACCGTC	135	1.1532393E-9	17.814816	22
ATCACGT	125	8.58563E-9	17.76	23
>>END_MODULE