FastQCFastQC Report
Wed 25 May 2016
SRR2088178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086246
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155181.428589840607008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152571.404562134175868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130471.2011091410233041No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64880.5972864341963054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51870.47751614275219423No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT44540.4100360323536289No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41010.37753878955595693No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38910.3582061521975685No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT37890.3488160140520656No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32750.30149708261296243No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA32720.30122090207927116No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT28710.264304770742539No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27760.25555905384231564No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25900.2384358607534573No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA24340.22407447300151162No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24270.223430051756232No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20760.1911169293143542No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20180.18577743899632312No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.18099030974567454No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17870.16451153790209586No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16930.1558578811797696No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.1473883448132375No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15020.13827438720142582No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15010.13818232702352873No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13890.1278715870990549No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC13340.12280827731471508No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA13210.12161149500205294No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC13090.12050677286728789No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC12940.11912587019883157No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC12070.11111663472178493No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC12020.11065633383229949No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.10807864885118104No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG11630.10706598689431308No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA11240.10347563995632665No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA11110.10227885764366451No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10970.1009900151531053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.030.0298251
CGCGGTA250.005496212729.59999817
ACGTTTA706.5920176E-926.42857226
ATCACGT706.5920176E-926.42857223
GTATTGG2700.024.6666681
GTGACGG400.001931119523.12519
TCACGTT802.7253918E-823.12524
TACCGTG753.7403697E-722.1999997
TTGGACC6100.021.5327854
GTTATCA1301.8189894E-1221.3461531
GACCCTC6100.021.2295077
TAGCCGA450.003825674320.55555722
ATTGGAC3350.020.4328373
GGACCCT6300.020.2619046
CGAACAA555.1428436E-420.18181814
CGAATTA1101.7553248E-920.18181815
GGTGACG555.1428436E-420.18181818
ACGTAGA656.901684E-519.92307727
ACGACGC656.901684E-519.92307727
CACGTTT951.6759077E-719.47368425
Produced by FastQC (version 0.11.5)