##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086246
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60420659776883	31.0	31.0	33.0	28.0	34.0
2	31.00148585127126	31.0	31.0	34.0	28.0	34.0
3	31.17867683747512	31.0	31.0	34.0	28.0	34.0
4	35.20966429335528	37.0	35.0	37.0	33.0	37.0
5	35.070967349937305	35.0	35.0	37.0	32.0	37.0
6	35.1017430674083	37.0	35.0	37.0	32.0	37.0
7	34.91056169596942	36.0	35.0	37.0	32.0	37.0
8	35.032952020076486	37.0	35.0	37.0	32.0	37.0
9	36.50981453556561	38.0	35.0	39.0	32.0	39.0
10	36.39163688519912	38.0	35.0	39.0	32.0	39.0
11	36.44049506281266	38.0	35.0	39.0	32.0	39.0
12	36.26528521163714	38.0	35.0	39.0	32.0	39.0
13	36.334744615860494	38.0	35.0	39.0	32.0	39.0
14	37.212486858409605	39.0	36.0	41.0	32.0	41.0
15	37.30556061886534	39.0	36.0	41.0	32.0	41.0
16	37.27467811158798	39.0	36.0	41.0	32.0	41.0
17	37.3840686179742	39.0	36.0	41.0	32.0	41.0
18	37.403806320115336	39.0	36.0	41.0	32.0	41.0
19	37.51700811786649	39.0	36.0	41.0	32.0	41.0
20	37.490109975088515	39.0	36.0	41.0	32.0	41.0
21	37.380847432349576	39.0	36.0	41.0	32.0	41.0
22	37.338995954875784	39.0	36.0	41.0	32.0	41.0
23	37.20758189213125	39.0	36.0	41.0	31.0	41.0
24	37.12372611728835	39.0	36.0	40.0	31.0	41.0
25	37.03099666189795	39.0	36.0	40.0	31.0	41.0
26	36.761366209864065	39.0	35.0	40.0	31.0	41.0
27	36.62038065042357	38.0	35.0	40.0	30.0	41.0
28	36.47002704728027	38.0	35.0	40.0	30.0	41.0
29	36.32200164603598	38.0	35.0	40.0	30.0	41.0
30	36.13932019082234	38.0	35.0	40.0	30.0	41.0
31	35.85065905881356	38.0	34.0	40.0	29.0	41.0
32	35.51809442796567	38.0	34.0	40.0	28.0	41.0
33	35.62538043868516	38.0	34.0	40.0	29.0	41.0
34	35.588302281435325	38.0	34.0	40.0	28.0	41.0
35	35.48122616792145	38.0	34.0	40.0	27.0	41.0
36	35.36247590324844	38.0	34.0	40.0	27.0	41.0
37	35.222349265267724	38.0	34.0	40.0	26.0	41.0
38	35.0691289081847	38.0	34.0	40.0	25.0	41.0
39	34.981175534823606	38.0	34.0	40.0	25.0	41.0
40	34.774519768082	38.0	33.0	40.0	24.0	41.0
41	34.6110153685261	38.0	33.0	40.0	23.0	41.0
42	34.36355576913517	38.0	33.0	40.0	22.0	41.0
43	33.99791483697063	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	1.0
16	19.0
17	25.0
18	69.0
19	165.0
20	425.0
21	772.0
22	1510.0
23	2388.0
24	3972.0
25	6290.0
26	9947.0
27	14467.0
28	20239.0
29	27053.0
30	35067.0
31	43452.0
32	53254.0
33	64994.0
34	79178.0
35	94990.0
36	118158.0
37	152191.0
38	179367.0
39	178250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.216899302736216	24.508904981007984	14.823069544099587	21.451126172156215
2	17.07532179635184	23.602204288899568	36.973300707206285	22.349173207542307
3	19.585158426360145	26.046586132423045	33.27321803716654	21.09503740405028
4	12.879034767446784	15.99453530784003	35.4302800654732	35.69614985923999
5	10.699049754843747	41.12328146662911	34.74387937907251	13.433789399454636
6	30.916017182111606	36.37279216678358	15.530552011238708	17.180638639866107
7	23.425816988048748	36.51999639124103	21.429307909994606	18.62487871071562
8	27.564474345590224	33.79252950068401	20.46497754652261	18.178018607203157
9	24.83507419129737	13.972893801219982	25.07148472813709	36.12054727934556
10	16.138885666782663	28.62298227105094	33.881091391821	21.35704067034539
11	30.42671733658858	22.322015455062665	25.72907057885599	21.52219662949277
12	19.85747243257973	28.334557733699366	31.902257867923105	19.905711965797803
13	30.705475555260964	21.722427516418932	29.634263325250448	17.937833603069656
14	20.286472861580158	23.60257252961116	29.486690860081417	26.624263748727266
15	22.336929203881994	29.932446241459115	27.72235755068373	20.00826700397516
16	21.056556249689294	29.082822859646896	27.923877280100456	21.936743610563354
17	18.34961877880333	27.962726675173027	28.757574251136482	24.930080294887162
18	19.419450106145387	27.19761453667033	33.94746678008481	19.435468577099478
19	18.640160700246536	25.70596347420382	35.46425027111722	20.18962555443242
20	23.484367261191295	24.552541505331206	33.77107947923398	18.192011754243513
21	22.767402595728775	25.54936911160087	32.20080902484336	19.48241926782699
22	20.713908267556334	25.349690585742085	33.11202066566874	20.824380481032843
23	19.76292662987942	27.56659172968186	34.237088099749045	18.43339354068968
24	18.274865914350894	26.603366088344625	32.55321538583341	22.568552611471066
25	19.565365488112267	27.571655039466197	33.98125286537304	18.881726607048495
26	21.11252883785073	26.4240328618011	32.27767927338743	20.185759026960742
27	19.588932893653922	27.95306035649383	31.952246544521223	20.50576020533102
28	19.289645255310493	27.597892190166867	32.00637792912471	21.10608462539793
29	18.26888200278758	27.35522156123015	33.21273450028815	21.163161935694124
30	17.67656681819772	27.51080326187622	35.570119475698874	19.242510444227182
31	19.37001379061465	26.786934083071422	34.07165596006798	19.771396166245953
32	20.51551858418811	27.181135764826752	31.899864303297782	20.403481347687354
33	21.41457828153107	27.150111484875435	31.211070052271765	20.224240181321726
34	19.457286839261087	30.06160667104873	31.32669763571051	19.154408853979668
35	18.070032018529872	30.748283537983106	31.52840148548303	19.653282958003988
36	19.052682357403388	26.83701481984744	34.027467074677375	20.0828357480718
37	19.020829535850993	27.125439357199017	33.048775323453434	20.804955783496556
38	19.469991143810887	26.59195062628539	33.782402881115324	20.155655348788397
39	20.585024018500413	25.950383246520587	33.63197655043149	19.832616184547515
40	18.708377292068278	26.81013324790149	32.82460879027403	21.656880669756205
41	19.44071600723961	26.142236657258117	32.74120226909926	21.675845066403006
42	16.79140820771722	27.96079341143719	33.155657189991956	22.09214119085364
43	17.396335636678984	27.778146018489362	32.841455802829195	21.984062542002455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	165.0
2	209.0
3	574.5
4	940.0
5	940.0
6	1597.0
7	2254.0
8	2542.0
9	2830.0
10	4268.0
11	5706.0
12	5706.0
13	10223.5
14	14741.0
15	24305.5
16	33870.0
17	33268.5
18	32667.0
19	32667.0
20	34658.5
21	36650.0
22	30204.5
23	23759.0
24	25642.0
25	27525.0
26	27525.0
27	29781.0
28	32037.0
29	32520.0
30	33003.0
31	35121.5
32	37240.0
33	37240.0
34	39946.0
35	42652.0
36	45502.0
37	48352.0
38	49530.0
39	50708.0
40	50708.0
41	53055.0
42	55402.0
43	63247.0
44	71092.0
45	90964.5
46	110837.0
47	110837.0
48	112443.0
49	114049.0
50	101275.5
51	88502.0
52	80783.5
53	73065.0
54	73065.0
55	62422.5
56	51780.0
57	40873.0
58	29966.0
59	25372.5
60	20779.0
61	20779.0
62	17855.0
63	14931.0
64	12856.5
65	10782.0
66	8944.5
67	7107.0
68	7107.0
69	6074.0
70	5041.0
71	4168.5
72	3296.0
73	2614.0
74	1932.0
75	1932.0
76	1502.5
77	1073.0
78	856.5
79	640.0
80	502.0
81	364.0
82	364.0
83	266.0
84	168.0
85	129.0
86	90.0
87	71.0
88	52.0
89	52.0
90	37.0
91	22.0
92	15.5
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1086246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.05074281906275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29861176549613	33.610105243333855
2	12.828577789496077	11.302167618788642
3	4.4905844361160625	5.934407403079038
4	2.0420248737354516	3.5981085017219785
5	1.090301591620752	2.4014297503850264
6	0.6379946133142765	1.686248197863275
7	0.45022630676934394	1.3882962274910977
8	0.2971788773639571	1.047276023841397
9	0.2246486232547232	0.8906344854885297
>10	1.3988152278621757	11.715249185992233
>50	0.1349666248587712	4.067430984341646
>100	0.08687979986800526	7.379745760717765
>500	0.0105436649693752	3.32467090009088
>1k	0.007591438777950144	6.4990437499359714
>5k	4.2174659877500795E-4	1.0845053996412792
>10k+	6.32619898162512E-4	4.070680567287378
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15518	1.428589840607008	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15257	1.404562134175868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13047	1.2011091410233041	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6488	0.5972864341963054	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5187	0.47751614275219423	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4454	0.4100360323536289	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4101	0.37753878955595693	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3891	0.3582061521975685	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3789	0.3488160140520656	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3275	0.30149708261296243	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3272	0.30122090207927116	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2871	0.264304770742539	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2776	0.25555905384231564	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2590	0.2384358607534573	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2434	0.22407447300151162	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2427	0.223430051756232	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2076	0.1911169293143542	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2018	0.18577743899632312	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1966	0.18099030974567454	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1787	0.16451153790209586	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1693	0.1558578811797696	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1601	0.1473883448132375	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1502	0.13827438720142582	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1501	0.13818232702352873	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1389	0.1278715870990549	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1334	0.12280827731471508	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1321	0.12161149500205294	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1309	0.12050677286728789	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1294	0.11912587019883157	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1207	0.11111663472178493	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1202	0.11065633383229949	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1174	0.10807864885118104	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1163	0.10706598689431308	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1124	0.10347563995632665	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.10227885764366451	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1097	0.1009900151531053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.206017789708776E-5	0.0	0.0	0.0
3	0.0	9.206017789708776E-5	0.0	0.0	0.0
4	0.0	9.206017789708776E-5	0.0	0.0	0.0
5	0.0	9.206017789708776E-5	0.0	0.0	0.0
6	0.0	9.206017789708776E-5	0.0	0.0	0.0
7	0.0	9.206017789708776E-5	0.0	0.0	0.0
8	0.0	9.206017789708776E-5	0.0	0.0	0.0
9	0.0	9.206017789708776E-5	0.0	1.8412035579417553E-4	0.0
10	0.0	1.8412035579417553E-4	0.0	2.7618053369126333E-4	0.0
11	0.0	1.8412035579417553E-4	0.0	3.6824071158835105E-4	0.0
12	0.0	1.8412035579417553E-4	0.0	5.523610673825267E-4	0.0
13	0.0	1.8412035579417553E-4	0.0	9.206017789708777E-4	0.0
14	0.0	1.8412035579417553E-4	0.0	0.0011047221347650533	0.0
15	0.0	1.8412035579417553E-4	0.0	0.0014729628463534042	0.0
16	0.0	1.8412035579417553E-4	0.0	0.002301504447427194	0.0
17	0.0	1.8412035579417553E-4	0.0	0.004050647827471862	0.0
18	0.0	1.8412035579417553E-4	0.0	0.004787129250648564	0.0
19	0.0	1.8412035579417553E-4	0.0	0.005339490318031091	0.0
20	0.0	1.8412035579417553E-4	0.0	0.0066283328085903195	0.0
21	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.009942499212885479	0.0
22	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.015374049708813658	0.0
23	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.02476418785431661	0.0
24	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.04170326058738076	0.0
25	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.050172796953912836	0.0
26	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.06379770328268182	0.0
27	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.16966690786433275	0.0
28	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.28317710721144196	0.0
29	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.4039600606124211	0.0
30	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.6486560134628804	0.0
31	9.206017789708776E-5	1.8412035579417553E-4	0.0	0.9926848982642974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3185	0.0	30.029825	1
CGCGGTA	25	0.0054962127	29.599998	17
ACGTTTA	70	6.5920176E-9	26.428572	26
ATCACGT	70	6.5920176E-9	26.428572	23
GTATTGG	270	0.0	24.666668	1
GTGACGG	40	0.0019311195	23.125	19
TCACGTT	80	2.7253918E-8	23.125	24
TACCGTG	75	3.7403697E-7	22.199999	7
TTGGACC	610	0.0	21.532785	4
GTTATCA	130	1.8189894E-12	21.346153	1
GACCCTC	610	0.0	21.229507	7
TAGCCGA	45	0.0038256743	20.555557	22
ATTGGAC	335	0.0	20.432837	3
GGACCCT	630	0.0	20.261904	6
CGAACAA	55	5.1428436E-4	20.181818	14
CGAATTA	110	1.7553248E-9	20.181818	15
GGTGACG	55	5.1428436E-4	20.181818	18
ACGTAGA	65	6.901684E-5	19.923077	27
ACGACGC	65	6.901684E-5	19.923077	27
CACGTTT	95	1.6759077E-7	19.473684	25
>>END_MODULE