FastQCFastQC Report
Wed 25 May 2016
SRR2088177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782702
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140241.7917419400998083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138561.7702778324317558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118471.5136028782346282No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64490.8239406568527997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.5622829633755887No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT39750.5078561189315985No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT37930.48460333562454166No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA37190.4751489072469471No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32280.4124174973361509No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA32190.4112676344253624No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT29920.38226553656436296No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT29300.3743442587344864No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27000.3449588732365575No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA24160.30867431027389736No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT23290.29755896880294164No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23220.2966646309834394No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA18860.24096016108301754No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18270.23342217089007056No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18230.23291112070749787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.2306113948859208No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17740.22665075597098258No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG14620.1867888417303137No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.16890208534027No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12880.16455815878840221No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC12780.16328053333197054No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12630.161364095147323No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12300.1571479311410984No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11860.15152637913279896No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11520.14718245258093118No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11250.14373286384856562No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT11000.14053880020748638No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA10760.1374724991120503No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10680.13645039874690496No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG10620.13568382347304594No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC10590.13530053583611643No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA10580.13517277329047325No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.13133989692117817No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT9880.1262293950954514No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC9730.12431295691080387No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAACTGTCTCT9350.11945798017636342No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA9050.11562510380706834No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8950.11434747835063663No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG8920.11396419071370713No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT8900.11370866562242078No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG8840.11294209034856179No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8680.11089788961827106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8680.11089788961827106No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA8520.10885368888798035No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA8480.10834263870540767No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT8360.10680948815768965No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG8330.10642620052076013No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCCTGTCT8310.1061706754294738No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA8220.10502081251868528No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG8120.10374318706225358No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA7900.10093241105810385No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.100293598329888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCACA200.001841577237.01
GGTATCA27950.029.652951
GTCCGCT250.00549528529.69
ATTGGAC1700.026.1176473
AATTACG400.001930639623.12516
GTATTGG1850.023.01
GTTATCA903.8235157E-922.611111
GGACCCT4050.022.3827156
TATTGGA2000.021.2750022
TTGGACC4300.021.0813944
GACCCTC4400.020.6022727
ATTACGT450.003824734120.55555517
CGTTAAA450.003824734120.55555521
TACGTTA450.003824734120.55555519
TGGACCC4400.020.1818185
GCGCGTG500.007032925318.513
ATAATAC500.007032925318.53
AAGACGT609.2332275E-418.55
TAAGTCG701.2185664E-418.55
GTACGGG609.2332275E-418.537
Produced by FastQC (version 0.11.5)