##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	782702
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59028979100603	31.0	31.0	33.0	28.0	34.0
2	30.966141647779104	31.0	31.0	34.0	28.0	34.0
3	31.139616354627943	31.0	31.0	34.0	28.0	34.0
4	35.18376470227494	35.0	35.0	37.0	33.0	37.0
5	35.05948624125146	35.0	35.0	37.0	32.0	37.0
6	35.10883324688068	37.0	35.0	37.0	32.0	37.0
7	34.914241946487934	36.0	35.0	37.0	32.0	37.0
8	35.04426716681444	37.0	35.0	37.0	32.0	37.0
9	36.48885399551809	38.0	35.0	39.0	32.0	39.0
10	36.377721022815834	38.0	35.0	39.0	32.0	39.0
11	36.42748070146748	38.0	35.0	39.0	32.0	39.0
12	36.25528489770053	38.0	35.0	39.0	32.0	39.0
13	36.32525661107292	38.0	35.0	39.0	32.0	39.0
14	37.182524127956746	39.0	36.0	41.0	32.0	41.0
15	37.24954069364841	39.0	36.0	41.0	32.0	41.0
16	37.216896085611125	39.0	36.0	41.0	32.0	41.0
17	37.37624792066457	39.0	36.0	41.0	32.0	41.0
18	37.411539768647586	39.0	36.0	41.0	32.0	41.0
19	37.53643021226469	39.0	36.0	41.0	32.0	41.0
20	37.51157528663527	39.0	36.0	41.0	32.0	41.0
21	37.40709746493557	39.0	36.0	41.0	32.0	41.0
22	37.3583266683872	39.0	36.0	41.0	32.0	41.0
23	37.2339638840836	39.0	36.0	41.0	31.0	41.0
24	37.13585374765875	39.0	36.0	41.0	31.0	41.0
25	37.051796213629196	39.0	36.0	40.0	31.0	41.0
26	36.76390631428053	38.0	35.0	40.0	31.0	41.0
27	36.611876550717895	38.0	35.0	40.0	30.0	41.0
28	36.42190897685198	38.0	35.0	40.0	30.0	41.0
29	36.27259672263518	38.0	35.0	40.0	30.0	41.0
30	36.07623974386165	38.0	35.0	40.0	30.0	41.0
31	35.740297073471126	38.0	34.0	40.0	29.0	41.0
32	35.42337952375234	38.0	34.0	40.0	28.0	41.0
33	35.4653060807306	38.0	34.0	40.0	28.0	41.0
34	35.40437612271337	38.0	34.0	40.0	27.0	41.0
35	35.26012198767858	38.0	34.0	40.0	27.0	41.0
36	35.123035331454375	38.0	34.0	40.0	26.0	41.0
37	34.94301279414132	38.0	34.0	40.0	25.0	41.0
38	34.76173946150642	38.0	33.0	40.0	24.0	41.0
39	34.613635840971405	38.0	33.0	40.0	23.0	41.0
40	34.352783051531745	38.0	33.0	40.0	22.0	41.0
41	34.15994976376705	38.0	33.0	40.0	21.0	41.0
42	33.87828062276575	38.0	33.0	40.0	18.0	41.0
43	33.49691325689726	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	6.0
17	13.0
18	61.0
19	143.0
20	291.0
21	646.0
22	1120.0
23	1919.0
24	3247.0
25	5009.0
26	7651.0
27	10946.0
28	15404.0
29	20718.0
30	26361.0
31	31915.0
32	38908.0
33	47846.0
34	57630.0
35	68697.0
36	84943.0
37	110350.0
38	123989.0
39	124887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.565249609685424	24.555961272617164	15.260469501802728	20.618319615894684
2	17.19901060684654	23.453881553899187	37.21045813093617	22.13664970831811
3	20.16016312721828	25.893124075318575	33.53932914442534	20.40738365303781
4	12.6423594164829	15.38184392016374	34.445804405763624	37.52999225758973
5	10.176031235387159	41.94061085828323	34.192451277753214	13.690906628576393
6	30.925051935474805	35.38485911624092	15.090034265914742	18.600054682369535
7	22.97796607137838	37.674363934166514	20.419393332328266	18.928276662126837
8	28.502546307534672	33.26080679492323	19.99496615570166	18.241680741840444
9	24.863230194888988	13.745461235566026	25.676949848090334	35.71435872145465
10	16.64758745985062	28.5458578105077	32.9162312093236	21.890323520318077
11	29.797802995265123	21.7057066418637	26.320745315586265	22.175745047284916
12	20.177283308334463	28.614210772426794	31.81044637678197	19.39805954245677
13	31.75410309415333	21.274124762681073	30.50318000976106	16.468592133404538
14	20.356534159871828	23.739941893594242	29.90806207215517	25.995461874378755
15	22.4902964346584	29.38512997283768	28.76509833883138	19.359475253672535
16	20.664058607234935	28.88366198118824	28.5038239329911	21.94845547858572
17	17.737274211641214	27.535256074470233	28.79256728614466	25.934902427743893
18	19.01949911971606	26.654716609897513	35.20254196360811	19.123242306778316
19	17.988455376375683	24.81008097590143	36.966303906212076	20.235159741510817
20	24.141244049459438	22.87205092104019	35.385881216606066	17.600823812894305
21	23.068038666056815	24.40354055566486	33.52578631458716	19.002634463691162
22	20.465260086214165	24.12757345707562	34.514412892774004	20.89275356393621
23	19.386944200985816	26.731246374737765	36.10825064967255	17.773558774603874
24	17.22034695196895	25.724860802706523	34.03709202225112	23.017700223073405
25	18.94922971961232	26.966201696175556	35.807625379774166	18.27694320443796
26	21.16527107379309	25.409287314967894	33.651121371863105	19.774320239375907
27	19.287417177929786	27.390756635347806	32.96912490321987	20.352701283502533
28	18.68335586212888	26.678863731024066	33.267194922205384	21.370585484641662
29	17.419017710444077	26.619837434936922	34.5827658546931	21.3783789999259
30	16.617563261624476	26.600162002907872	37.81106985800471	18.971204877462945
31	18.695110016328055	25.957644160868377	35.70669296871606	19.640552854087506
32	20.57666902601501	26.317934539582115	32.85388308704974	20.251513347353143
33	21.80497813982844	26.19272724485181	32.08219731136499	19.920097303954762
34	18.99803501204801	30.2283627740826	32.13215246671147	18.641449747157925
35	17.084407603404618	31.119762055034993	32.42920038533184	19.36662995622855
36	18.342357627807264	26.011687717675436	35.78590574701483	19.860048907502474
37	18.581784638342562	26.373511246936893	34.41552468244619	20.629179432274352
38	19.324085028529375	25.79244718935176	35.18963794649816	19.693829835620708
39	20.675301711251535	25.170754642252096	34.71193378833834	19.44200985815802
40	18.11123518273877	26.248942764934803	33.700437714481374	21.939384337845055
41	19.353598176572948	25.647564462592403	33.37157692199585	21.627260438838793
42	16.008008156360916	27.80994554760305	34.10812799762873	22.073918298407314
43	16.871299677271807	27.736354321312582	33.571525305927416	21.820820695488194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	92.0
2	132.0
3	370.0
4	608.0
5	608.0
6	1205.0
7	1802.0
8	2066.5
9	2331.0
10	3527.0
11	4723.0
12	4723.0
13	8578.0
14	12433.0
15	21201.0
16	29969.0
17	28883.5
18	27798.0
19	27798.0
20	29129.0
21	30460.0
22	23862.5
23	17265.0
24	18298.5
25	19332.0
26	19332.0
27	19760.5
28	20189.0
29	19828.5
30	19468.0
31	20433.5
32	21399.0
33	21399.0
34	22826.5
35	24254.0
36	26788.5
37	29323.0
38	29966.5
39	30610.0
40	30610.0
41	32820.0
42	35030.0
43	43162.0
44	51294.0
45	71781.0
46	92268.0
47	92268.0
48	92795.0
49	93322.0
50	81779.0
51	70236.0
52	62587.5
53	54939.0
54	54939.0
55	46123.0
56	37307.0
57	27921.0
58	18535.0
59	15234.0
60	11933.0
61	11933.0
62	10216.5
63	8500.0
64	7322.0
65	6144.0
66	5071.5
67	3999.0
68	3999.0
69	3398.0
70	2797.0
71	2302.5
72	1808.0
73	1449.5
74	1091.0
75	1091.0
76	859.0
77	627.0
78	497.0
79	367.0
80	283.5
81	200.0
82	200.0
83	138.0
84	76.0
85	61.0
86	46.0
87	35.5
88	25.0
89	25.0
90	15.5
91	6.0
92	5.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	782702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.721992494507106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07364619904695	33.78183719428994
2	11.343245912691312	9.692121334906933
3	3.778056810762761	4.842183441396843
4	1.652945605138298	2.8246851894618743
5	0.9391416528030174	2.006100132116475
6	0.6005658715834289	1.539442239494662
7	0.3910323561590808	1.1693976969456374
8	0.2955517410798872	1.0101247411322758
9	0.22701029463874234	0.8728498893358975
>10	1.4550999540295864	11.897859026778153
>50	0.12623062638285792	3.679247477507895
>100	0.09301202187837344	8.118262542838117
>500	0.013287431696909366	4.1069312916353535
>1k	0.009965573772682024	8.501565808865118
>5k	3.019870840206674E-4	0.8320170860351334
>10k+	9.059612520620022E-4	5.12537490725969
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14024	1.7917419400998083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13856	1.7702778324317558	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11847	1.5136028782346282	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6449	0.8239406568527997	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4401	0.5622829633755887	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3975	0.5078561189315985	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3793	0.48460333562454166	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3719	0.4751489072469471	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3228	0.4124174973361509	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3219	0.4112676344253624	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2992	0.38226553656436296	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2930	0.3743442587344864	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2700	0.3449588732365575	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2416	0.30867431027389736	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2329	0.29755896880294164	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2322	0.2966646309834394	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1886	0.24096016108301754	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1827	0.23342217089007056	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1823	0.23291112070749787	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1805	0.2306113948859208	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1774	0.22665075597098258	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1462	0.1867888417303137	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1322	0.16890208534027	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1288	0.16455815878840221	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1278	0.16328053333197054	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1263	0.161364095147323	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1230	0.1571479311410984	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1186	0.15152637913279896	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1152	0.14718245258093118	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1125	0.14373286384856562	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	1100	0.14053880020748638	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1076	0.1374724991120503	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1068	0.13645039874690496	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1062	0.13568382347304594	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1059	0.13530053583611643	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1058	0.13517277329047325	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.13133989692117817	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	988	0.1262293950954514	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	973	0.12431295691080387	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAACTGTCTCT	935	0.11945798017636342	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	905	0.11562510380706834	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	895	0.11434747835063663	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	892	0.11396419071370713	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	890	0.11370866562242078	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	884	0.11294209034856179	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	868	0.11089788961827106	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	868	0.11089788961827106	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	852	0.10885368888798035	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	848	0.10834263870540767	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	836	0.10680948815768965	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG	833	0.10642620052076013	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCCTGTCT	831	0.1061706754294738	No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	822	0.10502081251868528	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	812	0.10374318706225358	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	790	0.10093241105810385	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	785	0.100293598329888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2776254564316944E-4	0.0	0.0	0.0	0.0
7	1.2776254564316944E-4	0.0	0.0	0.0	0.0
8	2.555250912863389E-4	0.0	0.0	0.0	0.0
9	2.555250912863389E-4	0.0	0.0	0.0	0.0
10	2.555250912863389E-4	0.0	0.0	5.110501825726778E-4	0.0
11	3.8328763692950827E-4	0.0	0.0	7.665752738590165E-4	0.0
12	3.8328763692950827E-4	0.0	0.0	0.001533150547718033	0.0
13	5.110501825726778E-4	0.0	0.0	0.0016609130933612026	0.0
14	5.110501825726778E-4	0.0	0.0	0.002044200730290711	0.0
15	5.110501825726778E-4	0.0	0.0	0.0028107760041497275	0.0
16	5.110501825726778E-4	0.0	0.0	0.00447168909751093	0.0
17	5.110501825726778E-4	0.0	0.0	0.007154702556017488	0.0
18	5.110501825726778E-4	0.0	0.0	0.00868785310373552	0.0
19	5.110501825726778E-4	0.0	0.0	0.009965478560167216	0.0
20	5.110501825726778E-4	0.0	0.0	0.01354282983817596	0.0
21	5.110501825726778E-4	0.0	0.0	0.019419906937761754	0.0
22	5.110501825726778E-4	0.0	0.0	0.03372931204979673	0.0
23	5.110501825726778E-4	0.0	0.0	0.05774867063071258	0.0
24	5.110501825726778E-4	0.0	0.0	0.08675076849171204	0.0
25	5.110501825726778E-4	0.0	0.0	0.099527023056029	0.0
26	5.110501825726778E-4	0.0	0.0	0.1137086656224208	0.0
27	5.110501825726778E-4	0.0	0.0	0.23546637162036127	0.0
28	5.110501825726778E-4	0.0	0.0	0.36016261616809464	0.0
29	5.110501825726778E-4	0.0	0.0	0.5032566672884444	0.0
30	5.110501825726778E-4	0.0	0.0	0.8271347204938789	0.0
31	6.388127282158471E-4	0.0	0.0	1.236613679280237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCACA	20	0.0018415772	37.0	1
GGTATCA	2795	0.0	29.65295	1
GTCCGCT	25	0.005495285	29.6	9
ATTGGAC	170	0.0	26.117647	3
AATTACG	40	0.0019306396	23.125	16
GTATTGG	185	0.0	23.0	1
GTTATCA	90	3.8235157E-9	22.61111	1
GGACCCT	405	0.0	22.382715	6
TATTGGA	200	0.0	21.275002	2
TTGGACC	430	0.0	21.081394	4
GACCCTC	440	0.0	20.602272	7
ATTACGT	45	0.0038247341	20.555555	17
CGTTAAA	45	0.0038247341	20.555555	21
TACGTTA	45	0.0038247341	20.555555	19
TGGACCC	440	0.0	20.181818	5
GCGCGTG	50	0.0070329253	18.5	13
ATAATAC	50	0.0070329253	18.5	3
AAGACGT	60	9.2332275E-4	18.5	5
TAAGTCG	70	1.2185664E-4	18.5	5
GTACGGG	60	9.2332275E-4	18.5	37
>>END_MODULE