FastQCFastQC Report
Wed 25 May 2016
SRR2088176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862260
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122181.4169739985619187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118571.375107276227588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT103771.2034653120868415No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61360.711618305383527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.45218379607079073No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT37040.4295688075522464No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35680.413796302739313No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34620.40150302692923245No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT32240.3739011435065989No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA28940.33562962447521627No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT27580.31985711966228286No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT25510.2958504395425973No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG24170.2803098833298541No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA21670.2513163083060794No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA20850.24180641569828126No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT20300.2354278291930508No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA18450.21397258367545752No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17960.20828984297079767No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC15570.18057198524806903No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15560.18045601094797392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15240.17674483334493077No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12580.14589566951963445No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12160.1410247489156403No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12000.13916916011411873No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11760.13638577691183634No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.13429823951012454No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC10720.12432444970194605No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC10420.12084522069909308No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA10230.1186417089972862No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC10190.1181778117969058No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA9720.11272701969243615No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC9580.11110337949110478No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG9550.11075545659081948No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC9430.10936376498967829No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA8980.10414492148539883No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA8870.10286920418435275No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT8840.10252128128406746No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA8700.10089764108273606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.031.098161
CCGTATC250.0054955929.69
CGAACCT1350.023.29629732
GTATTGG1900.022.3947371
TATTGGA2250.020.5555532
TAGAACC555.141662E-420.1818184
TGCGTTC759.261372E-619.73333432
TCGAACC1600.019.6562531
ATTGGAC2050.018.9512213
CGTCTGT3250.018.78461534
TTCACGG500.007033489618.530
ATCACGT500.007033489618.523
ACGCTCC500.007033489618.527
TAGATTG500.007033489618.55
GTATCAA43950.017.5529022
CTAGTAC752.0665121E-417.2666663
ATCCACG752.0665121E-417.2666661
TTGGACC4650.017.1075274
GGACCCT4700.016.5319146
GCGTCTG3850.016.33766233
Produced by FastQC (version 0.11.5)