##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	862260
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.540003015331802	31.0	31.0	33.0	28.0	34.0
2	30.949953610279962	31.0	31.0	34.0	28.0	34.0
3	31.139238744694175	31.0	31.0	34.0	28.0	34.0
4	35.18409528448496	35.0	35.0	37.0	33.0	37.0
5	35.049043212024216	35.0	35.0	37.0	32.0	37.0
6	35.096142694778834	37.0	35.0	37.0	32.0	37.0
7	34.89619256372788	36.0	35.0	37.0	32.0	37.0
8	35.00799874747756	36.0	35.0	37.0	32.0	37.0
9	36.481336255885694	38.0	35.0	39.0	32.0	39.0
10	36.36732192146221	38.0	35.0	39.0	32.0	39.0
11	36.408286363741794	38.0	35.0	39.0	32.0	39.0
12	36.22409482058776	38.0	35.0	39.0	32.0	39.0
13	36.28907406118804	38.0	35.0	39.0	32.0	39.0
14	37.15482453088396	39.0	36.0	41.0	31.0	41.0
15	37.23129682462366	39.0	36.0	41.0	32.0	41.0
16	37.20092547491476	39.0	36.0	41.0	32.0	41.0
17	37.324245587177884	39.0	36.0	41.0	32.0	41.0
18	37.341334400296894	39.0	36.0	40.0	32.0	41.0
19	37.45016584324914	39.0	36.0	41.0	32.0	41.0
20	37.4269489481131	39.0	36.0	41.0	32.0	41.0
21	37.31175515505764	39.0	36.0	41.0	31.0	41.0
22	37.27007167211746	39.0	36.0	41.0	31.0	41.0
23	37.143822049033936	39.0	36.0	40.0	31.0	41.0
24	37.05796163570153	39.0	36.0	40.0	31.0	41.0
25	36.964596525409966	39.0	35.0	40.0	31.0	41.0
26	36.70633451627119	38.0	35.0	40.0	30.0	41.0
27	36.57028506482963	38.0	35.0	40.0	30.0	41.0
28	36.424554078816136	38.0	35.0	40.0	30.0	41.0
29	36.284126597545985	38.0	35.0	40.0	30.0	41.0
30	36.10672418991951	38.0	35.0	40.0	30.0	41.0
31	35.80964790202491	38.0	34.0	40.0	29.0	41.0
32	35.495239254981094	38.0	34.0	40.0	28.0	41.0
33	35.60619650685408	38.0	34.0	40.0	29.0	41.0
34	35.574153967480804	38.0	34.0	40.0	28.0	41.0
35	35.46861387516526	38.0	34.0	40.0	27.0	41.0
36	35.35702920232876	38.0	34.0	40.0	27.0	41.0
37	35.2251223528866	38.0	34.0	40.0	26.0	41.0
38	35.078811495372626	38.0	34.0	40.0	25.0	41.0
39	35.00783870294343	38.0	34.0	40.0	25.0	41.0
40	34.803831790875144	38.0	33.0	40.0	24.0	41.0
41	34.64333611671653	38.0	33.0	40.0	23.0	41.0
42	34.40851251362698	38.0	33.0	40.0	22.0	41.0
43	34.055518057198526	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	8.0
17	21.0
18	59.0
19	136.0
20	293.0
21	586.0
22	1086.0
23	1908.0
24	3177.0
25	4992.0
26	7756.0
27	11325.0
28	15937.0
29	21867.0
30	28289.0
31	34700.0
32	42830.0
33	52604.0
34	63498.0
35	76480.0
36	95335.0
37	121850.0
38	142997.0
39	134524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3515876881683	24.397397536705867	14.609978428780183	21.64103634634565
2	17.2290260478278	23.353744810150072	37.05761603228724	22.359613109734884
3	19.700090459954076	25.965370073991608	33.1638948808944	21.170644585159927
4	12.69234337670772	15.829796117180434	35.383758959014685	36.09410154709716
5	10.692946443068216	41.0435367522557	34.82777816435878	13.435738640317304
6	31.09201400969545	36.14130308723587	15.50216871941178	17.2645141836569
7	23.833066592443114	36.20149374898523	21.21274325609445	18.752696402477213
8	27.72910722983787	33.55275670911326	20.343399902581588	18.374736158467282
9	24.899334307517453	13.842460510750817	24.787071185025397	36.47113399670633
10	16.270382483241715	28.24240948205878	33.942662306032986	21.544545728666527
11	30.71416973998562	21.969823487115256	25.795235775752097	21.520770997147032
12	20.10901584208939	28.020782594577042	31.856168673022054	20.014032890311505
13	31.11544081831466	21.362466077517222	29.531463827615802	17.990629276552315
14	20.612808201702503	23.135249228770903	29.059448426228744	27.192494143297846
15	22.577528819613573	29.485885927678428	27.664857467585186	20.271727785122817
16	21.24567995732146	28.917031985711965	27.734905944842623	22.10238211212395
17	18.67603739011435	27.696518451511142	28.225477234244888	25.401966924129617
18	19.754598380998768	26.955790596803748	33.57467585183123	19.714935170366246
19	18.98081785076427	25.27137986222253	35.03340059842739	20.71440168858581
20	23.91598821701111	24.04263215271496	33.48804304966019	18.553336580613735
21	23.29575766010252	25.08187785586714	31.992322501333703	19.630041982696632
22	21.05467028506483	24.741841207988312	32.905736088882705	21.297752418064157
23	20.163639737434185	27.17985294458748	34.053533736923896	18.60297358105444
24	18.556351912416208	26.07716929928328	32.34302878482128	23.02345000347923
25	19.928211908241135	27.095655602718438	33.82587618583722	19.15025630320321
26	21.512652796140376	25.955860181383805	32.07814348340408	20.453343539071742
27	19.919165912833716	27.505160856354234	31.660751977361816	20.914921253450235
28	19.65381671421613	27.084406095609214	31.70273467399624	21.559042516178415
29	18.689954306125763	27.007515134646166	32.71808967132883	21.58444088789924
30	17.93043861480296	27.002528239742073	35.349778489086816	19.717254656368148
31	19.762484633405236	26.363393871917985	33.82088929093313	20.05323220374365
32	20.965833971191984	26.705981954398904	31.394822907243753	20.93336116716536
33	22.01111033794911	26.66991394706933	30.74826618421358	20.57070953076798
34	19.754946303899057	29.918702015633336	30.890102753229886	19.436248927237724
35	18.261893164474753	30.925474914758887	31.0032936701227	19.809338250643655
36	19.359705889174958	26.622248509730245	33.75130471087607	20.266740890218728
37	19.469185628464732	27.02630297126157	32.5626841092014	20.941827291072297
38	19.911163686127153	26.535847656159394	33.423561338807325	20.129427318906128
39	21.18061837496811	25.845452647693275	33.00303852666249	19.97089045067613
40	19.031846542806115	26.78588824716443	32.23702827453436	21.945236935495092
41	20.125484192702896	26.043652726555795	32.143437014357616	21.68742606638369
42	17.25697585415072	27.95734465242502	32.659638624080905	22.126040869343353
43	17.93844084150952	27.75067844965556	32.32308120520493	21.987799503629997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	98.0
2	131.0
3	364.0
4	597.0
5	597.0
6	1115.0
7	1633.0
8	1844.0
9	2055.0
10	3036.5
11	4018.0
12	4018.0
13	7522.5
14	11027.0
15	18214.0
16	25401.0
17	24997.5
18	24594.0
19	24594.0
20	26053.0
21	27512.0
22	22404.0
23	17296.0
24	18746.5
25	20197.0
26	20197.0
27	21797.0
28	23397.0
29	24084.0
30	24771.0
31	26552.0
32	28333.0
33	28333.0
34	30538.0
35	32743.0
36	35034.0
37	37325.0
38	38447.0
39	39569.0
40	39569.0
41	41560.0
42	43551.0
43	50317.0
44	57083.0
45	74095.5
46	91108.0
47	91108.0
48	92325.0
49	93542.0
50	83261.5
51	72981.0
52	66801.0
53	60621.0
54	60621.0
55	51726.0
56	42831.0
57	33591.0
58	24351.0
59	20653.0
60	16955.0
61	16955.0
62	14851.0
63	12747.0
64	10817.0
65	8887.0
66	7392.5
67	5898.0
68	5898.0
69	5041.5
70	4185.0
71	3538.0
72	2891.0
73	2325.5
74	1760.0
75	1760.0
76	1395.0
77	1030.0
78	785.0
79	540.0
80	432.0
81	324.0
82	324.0
83	232.5
84	141.0
85	115.5
86	90.0
87	72.5
88	55.0
89	55.0
90	37.0
91	19.0
92	11.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	862260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.090477831550075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96143883835566	37.9729332400324
2	11.779467480632775	11.329604394896709
3	3.8251604581107754	5.518613826386944
4	1.7275331148380706	3.3231157184955546
5	0.8541812668572861	2.053899263896284
6	0.5522958755389262	1.593610353543676
7	0.37162302287490967	1.251007012028163
8	0.280975930454341	1.0809813403770911
9	0.22565293834280856	0.9766581866099071
>10	1.2532613809737674	11.193612718884689
>50	0.08712550815264422	2.8838929840432805
>100	0.06303174814690402	6.080828392308528
>500	0.010951462032649847	3.7645731289608917
>1k	0.006327511396642133	6.226420911330236
>5k	2.433658229477744E-4	0.7181315898558736
>10k+	7.300974688433232E-4	4.03211693834983
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12218	1.4169739985619187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11857	1.375107276227588	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10377	1.2034653120868415	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6136	0.711618305383527	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3899	0.45218379607079073	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3704	0.4295688075522464	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3568	0.413796302739313	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3462	0.40150302692923245	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3224	0.3739011435065989	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2894	0.33562962447521627	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2758	0.31985711966228286	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2551	0.2958504395425973	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2417	0.2803098833298541	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2167	0.2513163083060794	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2085	0.24180641569828126	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2030	0.2354278291930508	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1845	0.21397258367545752	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1796	0.20828984297079767	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1557	0.18057198524806903	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1556	0.18045601094797392	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1524	0.17674483334493077	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1258	0.14589566951963445	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1216	0.1410247489156403	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1200	0.13916916011411873	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1176	0.13638577691183634	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1158	0.13429823951012454	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1072	0.12432444970194605	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1042	0.12084522069909308	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1023	0.1186417089972862	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1019	0.1181778117969058	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	972	0.11272701969243615	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	958	0.11110337949110478	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	955	0.11075545659081948	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	943	0.10936376498967829	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	898	0.10414492148539883	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	887	0.10286920418435275	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	884	0.10252128128406746	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	870	0.10089764108273606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1597430009509893E-4	0.0
2	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
3	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
4	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
5	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
6	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
7	0.0	1.1597430009509893E-4	0.0	1.1597430009509893E-4	0.0
8	0.0	1.1597430009509893E-4	0.0	2.3194860019019786E-4	0.0
9	0.0	1.1597430009509893E-4	0.0	2.3194860019019786E-4	0.0
10	0.0	1.1597430009509893E-4	0.0	3.479229002852968E-4	0.0
11	0.0	1.1597430009509893E-4	0.0	6.958458005705936E-4	0.0
12	0.0	1.1597430009509893E-4	0.0	9.277944007607914E-4	0.0
13	0.0	1.1597430009509893E-4	0.0	0.0010437687008558903	0.0
14	0.0	1.1597430009509893E-4	0.0	0.0013916916011411872	0.0
15	0.0	1.1597430009509893E-4	0.0	0.0018555888015215829	0.0
16	0.0	1.1597430009509893E-4	0.0	0.003015331802472572	0.0
17	0.0	1.1597430009509893E-4	0.0	0.0049868949040892535	0.0
18	0.0	1.1597430009509893E-4	0.0	0.005914689304850045	0.0
19	0.0	1.1597430009509893E-4	0.0	0.006262612205135342	0.0
20	0.0	1.1597430009509893E-4	0.0	0.007770278106371628	0.0
21	0.0	1.1597430009509893E-4	0.0	0.009973789808178507	0.0
22	0.0	1.1597430009509893E-4	0.0	0.01600445341312365	0.0
23	0.0	1.1597430009509893E-4	0.0	0.02481850022035117	0.0
24	0.0	1.1597430009509893E-4	0.0	0.03861944193166794	0.0
25	0.0	1.1597430009509893E-4	0.0	0.04534595133718368	0.0
26	0.0	1.1597430009509893E-4	0.0	0.055551689745552385	0.0
27	0.0	1.1597430009509893E-4	0.0	0.1420685176164962	0.0
28	0.0	1.1597430009509893E-4	0.0	0.2276575510866792	0.0
29	0.0	1.1597430009509893E-4	0.0	0.3239162201656113	0.0
30	0.0	1.1597430009509893E-4	0.0	0.5272191682323197	0.0
31	0.0	1.1597430009509893E-4	0.0	0.821793890473871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2445	0.0	31.09816	1
CCGTATC	25	0.00549559	29.6	9
CGAACCT	135	0.0	23.296297	32
GTATTGG	190	0.0	22.394737	1
TATTGGA	225	0.0	20.555553	2
TAGAACC	55	5.141662E-4	20.181818	4
TGCGTTC	75	9.261372E-6	19.733334	32
TCGAACC	160	0.0	19.65625	31
ATTGGAC	205	0.0	18.951221	3
CGTCTGT	325	0.0	18.784615	34
TTCACGG	50	0.0070334896	18.5	30
ATCACGT	50	0.0070334896	18.5	23
ACGCTCC	50	0.0070334896	18.5	27
TAGATTG	50	0.0070334896	18.5	5
GTATCAA	4395	0.0	17.552902	2
CTAGTAC	75	2.0665121E-4	17.266666	3
ATCCACG	75	2.0665121E-4	17.266666	1
TTGGACC	465	0.0	17.107527	4
GGACCCT	470	0.0	16.531914	6
GCGTCTG	385	0.0	16.337662	33
>>END_MODULE