FastQCFastQC Report
Wed 25 May 2016
SRR2088175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367002
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205111.5004367221115988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201711.475564776057387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165611.2114832311876647No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG79350.5804673292358021No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68030.4976583794317785No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT54550.3990484286050789No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50210.3673001209947023No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT45250.33101634086855763No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40010.2926842828320661No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA38360.280614073717522No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT37380.2734451010313079No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT34640.2534012386229135No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG34570.2528891691453268No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT30010.2195315003196777No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29120.2130209026760751No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27760.20307212425439025No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27650.202267443646754No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.1812725950656985No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23560.1723479555991871No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.15157256536566882No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20000.14630556502477685No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19240.14074595355383532No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC19200.14045334242378577No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA17000.12435973027106033No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16990.12428657748854792No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA16550.12106785505800284No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC16130.11799543819248252No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15940.11660553532474714No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.10833927090084725No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC14760.10797350698828531No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC14700.10753459029321098No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14020.10256020108236857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.030.5031131
ATTGGAC3300.023.5454523
GGTAACG400.001931372923.12500228
ACGTTTA802.726847E-823.12500226
TTGCGTG603.72695E-521.5833347
GTATTGG3900.021.3461551
TATTGGA3750.020.7200012
AACGCGG450.003826172620.5555576
ATCACGT1001.2903911E-820.3523
TACCCCG851.2456621E-619.5882345
ACCGCCG1355.638867E-1119.18518612
GGACCCT6850.018.905116
AGCCGTC902.1528213E-618.512
AGATCGG500.00703554618.517
TACTTCG500.00703554618.57
CAACGCG500.00703554618.55
TCCGACG902.1528213E-618.523
TTCGTTC500.00703554618.534
TATCGGC609.238033E-418.52
TTGGACC7300.018.2465744
Produced by FastQC (version 0.11.5)