##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1367002
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095977913711902	31.0	31.0	33.0	30.0	34.0
2	31.24571873340346	31.0	31.0	34.0	28.0	34.0
3	31.287878145021	31.0	31.0	34.0	28.0	34.0
4	35.2474261193473	37.0	35.0	37.0	33.0	37.0
5	35.12368379856065	37.0	35.0	37.0	32.0	37.0
6	35.18134355326474	37.0	35.0	37.0	32.0	37.0
7	35.00889757293698	37.0	35.0	37.0	32.0	37.0
8	35.07408328590595	37.0	35.0	37.0	32.0	37.0
9	36.550734380783645	39.0	35.0	39.0	32.0	39.0
10	36.41243319322137	38.0	35.0	39.0	32.0	39.0
11	36.45123708670506	38.0	35.0	39.0	32.0	39.0
12	36.28594032781225	38.0	35.0	39.0	32.0	39.0
13	36.33660301886903	38.0	35.0	39.0	32.0	39.0
14	37.24000915872837	39.0	36.0	41.0	32.0	41.0
15	37.306213158429905	39.0	36.0	41.0	32.0	41.0
16	37.27054239862122	39.0	36.0	41.0	32.0	41.0
17	37.394666576932586	39.0	36.0	41.0	32.0	41.0
18	37.425083503901234	39.0	36.0	41.0	32.0	41.0
19	37.52960419955494	39.0	36.0	41.0	32.0	41.0
20	37.493893205715864	39.0	36.0	41.0	32.0	41.0
21	37.37533668568151	39.0	36.0	41.0	32.0	41.0
22	37.3383316191198	39.0	36.0	41.0	31.0	41.0
23	37.21554467367275	39.0	36.0	41.0	31.0	41.0
24	37.139469437498995	39.0	36.0	41.0	31.0	41.0
25	37.051765835017065	39.0	36.0	40.0	31.0	41.0
26	36.76333172884897	39.0	35.0	40.0	30.0	41.0
27	36.60350387197678	38.0	35.0	40.0	30.0	41.0
28	36.441264899392976	38.0	35.0	40.0	30.0	41.0
29	36.29766013509856	38.0	35.0	40.0	30.0	41.0
30	36.109437294166355	38.0	35.0	40.0	30.0	41.0
31	35.80925046195982	38.0	34.0	40.0	29.0	41.0
32	35.50487343837098	38.0	34.0	40.0	28.0	41.0
33	35.60991863947529	38.0	34.0	40.0	29.0	41.0
34	35.563367134795705	38.0	34.0	40.0	28.0	41.0
35	35.46014343797595	38.0	34.0	40.0	27.0	41.0
36	35.348056550026996	38.0	34.0	40.0	27.0	41.0
37	35.193426198352306	38.0	34.0	40.0	26.0	41.0
38	35.04330059502473	38.0	34.0	40.0	25.0	41.0
39	34.928016930479984	38.0	34.0	40.0	24.0	41.0
40	34.728625122713794	38.0	33.0	40.0	24.0	41.0
41	34.54712721707796	38.0	33.0	40.0	23.0	41.0
42	34.29434850863422	38.0	33.0	40.0	21.0	41.0
43	33.95885155983678	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	5.0
16	17.0
17	32.0
18	106.0
19	237.0
20	480.0
21	1035.0
22	1838.0
23	3211.0
24	5279.0
25	8524.0
26	12646.0
27	18469.0
28	25793.0
29	34385.0
30	44500.0
31	53470.0
32	65726.0
33	79891.0
34	97386.0
35	116830.0
36	145470.0
37	191997.0
38	228994.0
39	230678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18545693422541	23.265218339109964	14.625435807701818	21.92388891896281
2	17.175614958866188	23.5376393011861	36.54969049057719	22.73705524937052
3	19.938815012706637	26.001863932898416	32.87405578045972	21.185265273935226
4	13.138971267050085	16.082419777001057	35.27141876895571	35.50719018699314
5	10.858945341703963	41.133224384455914	34.52679659576211	13.481033678078013
6	31.08934734550498	36.18948618948619	15.511096545579303	17.210069919429525
7	23.919131061988207	36.174563021853665	21.36946398030142	18.536841935856714
8	27.752263712854848	33.64384251083759	20.307870800481638	18.29602297582593
9	25.154388947492397	13.942554582948672	24.82066595367088	36.08239051588805
10	16.447744772867924	28.47852453763784	33.748450989830296	21.325279699663934
11	30.5355807818862	22.272169316504293	25.509472553807527	21.68277734780198
12	20.15073862364503	28.434340257000358	31.582982321898577	19.83193879745604
13	30.743627295351434	21.496822974655487	29.565648038554443	18.193901691438636
14	20.66471007357707	23.372314012708102	29.48115657475263	26.481819338962197
15	22.73083726285697	29.617440208573214	27.486865417899903	20.164857110669917
16	21.146421146421147	28.52183098488517	28.365064571961124	21.96668329673256
17	18.705824863460332	27.881378373989214	28.806322156075854	24.606474606474606
18	19.515187249177394	26.98394003812723	33.68992876382039	19.810943948874986
19	18.93552460054923	25.63792883989928	35.322625716714384	20.103920842837102
20	23.362657845416464	24.34261251995242	33.8405503430134	18.454179291617713
21	22.7015029970695	25.397841407693623	32.50821871511527	19.392436880121608
22	20.825426736756786	25.24619568954544	33.33857594941339	20.58980162428438
23	20.047446894737536	27.209177455482873	34.176394767527775	18.56698088225182
24	18.358641757656535	26.6265155427717	32.670544739510255	22.344297960061507
25	19.690680774424617	27.4148099271252	33.99373226959434	18.90077702885585
26	20.91438052029185	26.401936500458667	32.531042383259134	20.152640595990352
27	19.67209996766647	27.828781523362807	32.046551504679584	20.45256700429114
28	19.548471765220533	27.467041013839044	31.992125834490366	20.992361386450057
29	18.529453504822964	27.428489497455015	32.99110023247954	21.05095676524248
30	17.94437755028888	27.600691147489176	35.239158391867754	19.215772910354193
31	19.632524312327266	26.8314164865889	33.82423727251314	19.711821928570696
32	20.635595266137138	27.299960058580748	31.780129070769465	20.28431560451265
33	21.456662097056185	27.233171568146936	31.12965452866931	20.18051180612757
34	19.67422139835933	29.883570031353283	31.308878845824662	19.13332972446273
35	18.172614231727533	30.746333948304393	31.451526771723813	19.629525048244258
36	19.138157808108545	27.116785491169725	33.78166235309092	19.9633943476308
37	19.289291456779143	27.334780783056644	32.717435673100695	20.658492087063514
38	19.662516953157347	27.029221610502397	33.31999514265524	19.988266293685015
39	20.81079618025431	26.255118865956305	33.1189712963112	19.81511365747819
40	18.74042612958869	27.053435181514	32.54230791176604	21.66383077713127
41	19.66741818958568	26.501936354153106	32.334919773343415	21.4957256829178
42	17.09507374532005	28.299592831612536	32.67361715637578	21.931716266691637
43	17.655277753799922	27.916418556812644	32.40324447220999	22.025059217177446
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170.0
1	209.0
2	248.0
3	699.5
4	1151.0
5	1151.0
6	2082.5
7	3014.0
8	3276.0
9	3538.0
10	5388.0
11	7238.0
12	7238.0
13	13186.0
14	19134.0
15	30713.0
16	42292.0
17	41296.0
18	40300.0
19	40300.0
20	43308.0
21	46316.0
22	37551.0
23	28786.0
24	31383.0
25	33980.0
26	33980.0
27	36343.5
28	38707.0
29	39283.0
30	39859.0
31	42471.5
32	45084.0
33	45084.0
34	48612.5
35	52141.0
36	56716.0
37	61291.0
38	62968.5
39	64646.0
40	64646.0
41	67600.5
42	70555.0
43	79904.0
44	89253.0
45	113821.5
46	138390.0
47	138390.0
48	140256.5
49	142123.0
50	127248.0
51	112373.0
52	102263.0
53	92153.0
54	92153.0
55	79001.0
56	65849.0
57	52539.5
58	39230.0
59	33523.5
60	27817.0
61	27817.0
62	23816.5
63	19816.0
64	17014.5
65	14213.0
66	11838.5
67	9464.0
68	9464.0
69	8211.5
70	6959.0
71	5806.0
72	4653.0
73	3642.0
74	2631.0
75	2631.0
76	2156.0
77	1681.0
78	1292.5
79	904.0
80	727.0
81	550.0
82	550.0
83	406.0
84	262.0
85	186.0
86	110.0
87	89.5
88	69.0
89	69.0
90	55.0
91	41.0
92	24.0
93	7.0
94	5.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1367002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.341753385120406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37684975720023	29.19809023696883
2	13.973325600759148	11.2741691071163
3	5.42600046480236	6.566831178558165
4	2.6186698520840666	4.225669334793006
5	1.4965703753188788	3.0187136502295644
6	0.8879977419083289	2.1494031546365764
7	0.6100761005688818	1.7228077716714023
8	0.4100007378626371	1.3232118923657523
9	0.29769927287764547	1.0808739584423672
>10	1.6385624577471323	12.198376504689515
>50	0.14969471153359765	4.109288502823455
>100	0.09299432667843327	7.3012311842047355
>500	0.011144700340481121	3.0952156768984183
>1k	0.009135000279082888	6.658675316062242
>5k	7.30800022326631E-4	1.8583823806957191
>10k+	5.481000167449732E-4	4.219060149843937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20511	1.5004367221115988	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20171	1.475564776057387	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16561	1.2114832311876647	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7935	0.5804673292358021	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6803	0.4976583794317785	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5455	0.3990484286050789	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5021	0.3673001209947023	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4525	0.33101634086855763	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4001	0.2926842828320661	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3836	0.280614073717522	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3738	0.2734451010313079	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3464	0.2534012386229135	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3457	0.2528891691453268	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3001	0.2195315003196777	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2912	0.2130209026760751	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2776	0.20307212425439025	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2765	0.202267443646754	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2478	0.1812725950656985	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2356	0.1723479555991871	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2072	0.15157256536566882	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2000	0.14630556502477685	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1924	0.14074595355383532	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1920	0.14045334242378577	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1700	0.12435973027106033	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1699	0.12428657748854792	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1655	0.12106785505800284	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1613	0.11799543819248252	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1594	0.11660553532474714	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.10833927090084725	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1476	0.10797350698828531	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1470	0.10753459029321098	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1402	0.10256020108236857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.315278251238843E-5	0.0	0.0	0.0	0.0
6	1.4630556502477686E-4	0.0	0.0	0.0	0.0
7	1.4630556502477686E-4	0.0	0.0	0.0	0.0
8	1.4630556502477686E-4	0.0	0.0	0.0	0.0
9	1.4630556502477686E-4	0.0	0.0	0.0	0.0
10	1.4630556502477686E-4	0.0	0.0	0.0	0.0
11	1.4630556502477686E-4	0.0	0.0	1.4630556502477686E-4	0.0
12	1.4630556502477686E-4	0.0	0.0	1.4630556502477686E-4	0.0
13	1.4630556502477686E-4	0.0	0.0	2.1945834753716526E-4	0.0
14	1.4630556502477686E-4	0.0	0.0	2.926111300495537E-4	0.0
15	1.4630556502477686E-4	0.0	0.0	7.315278251238842E-4	0.0
16	1.4630556502477686E-4	0.0	0.0	0.0016825139977849337	0.0
17	1.4630556502477686E-4	0.0	0.0	0.0023408890403964298	0.0
18	1.4630556502477686E-4	0.0	0.0	0.0029261113004955368	0.0
19	1.4630556502477686E-4	0.0	0.0	0.0037307919081318096	0.0
20	1.4630556502477686E-4	0.0	0.0	0.004462319733255694	0.0
21	1.4630556502477686E-4	0.0	0.0	0.005486458688429132	0.0
22	1.4630556502477686E-4	0.0	0.0	0.008485722771437058	0.0
23	1.4630556502477686E-4	0.0	0.0	0.014191639807403355	0.0
24	1.4630556502477686E-4	0.0	0.0	0.024506182141650124	0.0
25	1.4630556502477686E-4	0.0	0.0	0.02977318248254209	0.0
26	1.4630556502477686E-4	0.0	0.0	0.04038033594683841	0.0
27	1.4630556502477686E-4	0.0	0.0	0.1232624385333745	0.0
28	1.4630556502477686E-4	0.0	0.0	0.2122893748509512	0.0
29	1.4630556502477686E-4	0.0	0.0	0.31609317323603037	0.0
30	1.4630556502477686E-4	0.0	0.0	0.5070219355933642	0.0
31	1.4630556502477686E-4	0.0	0.0	0.7981700099926701	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4015	0.0	30.503113	1
ATTGGAC	330	0.0	23.545452	3
GGTAACG	40	0.0019313729	23.125002	28
ACGTTTA	80	2.726847E-8	23.125002	26
TTGCGTG	60	3.72695E-5	21.583334	7
GTATTGG	390	0.0	21.346155	1
TATTGGA	375	0.0	20.720001	2
AACGCGG	45	0.0038261726	20.555557	6
ATCACGT	100	1.2903911E-8	20.35	23
TACCCCG	85	1.2456621E-6	19.588234	5
ACCGCCG	135	5.638867E-11	19.185186	12
GGACCCT	685	0.0	18.90511	6
AGCCGTC	90	2.1528213E-6	18.5	12
AGATCGG	50	0.007035546	18.5	17
TACTTCG	50	0.007035546	18.5	7
CAACGCG	50	0.007035546	18.5	5
TCCGACG	90	2.1528213E-6	18.5	23
TTCGTTC	50	0.007035546	18.5	34
TATCGGC	60	9.238033E-4	18.5	2
TTGGACC	730	0.0	18.246574	4
>>END_MODULE