FastQCFastQC Report
Wed 25 May 2016
SRR2088174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061591
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353261.7135309574013466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347241.6843302090472843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT298411.4474743050391663No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG144260.6997508235144605No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109950.533325960386905No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94030.45610404779609537No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT93530.4536787364710071No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT78140.3790276538847909No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72230.3503604740222479No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70090.3399801415508702No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT69220.33576009984521665No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62230.301854247520483No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59740.2897761971215435No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT51900.2517473155441598No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51490.24975856025758747No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA51440.24951602912507861No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA49550.2403483523162451No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43870.21279681566324263No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.20605445017949728No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC36590.17748428276995776No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35520.1722941165342689No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34480.16724946897808537No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.16230183387490535No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30890.14983573366395178No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA30610.14847755932190237No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC28980.14057104440211468No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28620.13882482024805115No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA27250.13217946721730936No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC26610.1290750687211964No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC26500.12854150022967697No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26250.12732884456713287No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC26140.12679527607561344No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.1245154834300305No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA23810.11549332530070222No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG23790.11539631284769869No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA23710.11500826303568457No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23500.1139896322791475No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA22150.10744129170140926No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC22090.10715025434239867No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT21490.10423988075229276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC21070.10220261923921865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69250.031.977621
GTATTGG4350.022.9655151
ATCACGT450.00382682320.55555523
CTAACGC656.905526E-519.9230773
TCTAACG656.905526E-519.9230772
ATTGGAC4850.019.8350523
CAATGCG759.2716255E-619.73333419
CACGGCT2950.019.4406764
ATCCACG3150.018.7936521
TATTGGA5550.018.6666662
CGTGACT500.007036729718.512
AATGCGT500.007036729718.535
TCTGTCG609.2402095E-418.58
TCCACGG3250.018.2153842
ACGGCTC3050.018.1967225
GTATCAA123550.017.953462
TTTTTAC16500.017.7151531
CGTCTGT10450.017.52631634
CTCTCGA953.6091988E-617.5263161
CGACGAG752.0682698E-417.26666624
Produced by FastQC (version 0.11.5)