##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2061591
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0797747952916	31.0	31.0	33.0	30.0	34.0
2	31.214681767625102	31.0	31.0	34.0	28.0	34.0
3	31.249110517071525	31.0	31.0	34.0	28.0	34.0
4	35.225081017524815	37.0	35.0	37.0	33.0	37.0
5	35.110011151581475	37.0	35.0	37.0	32.0	37.0
6	35.17351986887797	37.0	35.0	37.0	32.0	37.0
7	34.99841627170472	37.0	35.0	37.0	32.0	37.0
8	35.06366296709677	37.0	35.0	37.0	32.0	37.0
9	36.52198423450626	39.0	35.0	39.0	32.0	39.0
10	36.387172819438966	38.0	35.0	39.0	32.0	39.0
11	36.42620966040306	38.0	35.0	39.0	32.0	39.0
12	36.26828454334541	38.0	35.0	39.0	32.0	39.0
13	36.31273564931163	38.0	35.0	39.0	32.0	39.0
14	37.19557710525512	39.0	36.0	41.0	31.0	41.0
15	37.25882000843038	39.0	36.0	41.0	32.0	41.0
16	37.23129563526422	39.0	36.0	41.0	32.0	41.0
17	37.37975088172193	39.0	36.0	41.0	32.0	41.0
18	37.41316876140806	39.0	36.0	41.0	32.0	41.0
19	37.52313625738568	39.0	36.0	41.0	32.0	41.0
20	37.49134818691001	39.0	36.0	41.0	32.0	41.0
21	37.37904366093954	39.0	36.0	41.0	32.0	41.0
22	37.34435055255868	39.0	36.0	41.0	32.0	41.0
23	37.216617651124785	39.0	36.0	41.0	31.0	41.0
24	37.13607742757899	39.0	36.0	41.0	31.0	41.0
25	37.04484594664994	39.0	36.0	40.0	31.0	41.0
26	36.75122757132719	39.0	35.0	40.0	30.0	41.0
27	36.58460577292004	38.0	35.0	40.0	30.0	41.0
28	36.40945463964482	38.0	35.0	40.0	30.0	41.0
29	36.26152762599371	38.0	35.0	40.0	30.0	41.0
30	36.05387974627363	38.0	35.0	40.0	30.0	41.0
31	35.7402831114416	38.0	34.0	40.0	29.0	41.0
32	35.43577702851827	38.0	34.0	40.0	27.0	41.0
33	35.49058421384261	38.0	34.0	40.0	28.0	41.0
34	35.430398173061484	38.0	34.0	40.0	27.0	41.0
35	35.320492765053785	38.0	34.0	40.0	27.0	41.0
36	35.196122800303264	38.0	34.0	40.0	26.0	41.0
37	35.01661726307498	38.0	34.0	40.0	25.0	41.0
38	34.84893269324517	38.0	33.0	40.0	25.0	41.0
39	34.7123488606615	38.0	33.0	40.0	24.0	41.0
40	34.47876761200452	38.0	33.0	40.0	23.0	41.0
41	34.279096096170385	38.0	33.0	40.0	21.0	41.0
42	33.99613502387234	38.0	33.0	40.0	19.0	41.0
43	33.645530078468525	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	1.0
15	5.0
16	39.0
17	72.0
18	168.0
19	379.0
20	831.0
21	1704.0
22	3047.0
23	5198.0
24	8447.0
25	13621.0
26	20105.0
27	28996.0
28	40993.0
29	54167.0
30	68303.0
31	83156.0
32	100459.0
33	121804.0
34	147267.0
35	175386.0
36	219349.0
37	289815.0
38	335390.0
39	342886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28626434632282	23.42855590657895	15.043478556124857	21.24170119097338
2	17.107709531133963	23.641837784507207	36.7569027998279	22.49354988453093
3	19.871254773619015	26.066275997518424	33.21662735237009	20.84584187649248
4	12.81267719930869	15.760934152312462	34.75204344605695	36.674345202321895
5	10.524395964087931	41.607234412645376	34.26567151292376	13.602698110342933
6	31.18475003043766	35.702425941906036	15.285912676180677	17.826911351475633
7	23.639315460729115	36.83334861279468	20.882415571274805	18.644920355201393
8	28.34737831121692	33.17801639607468	20.002561128759293	18.472044163949107
9	24.97900893048136	13.808898079201937	25.400722063687702	35.811370926628996
10	16.507590496854128	28.73853252172715	33.32644544917008	21.427431532248637
11	30.274967246170554	22.056751314882536	25.93788001596825	21.73040142297866
12	20.44333720898083	28.756673850438812	31.52487569066803	19.275113249912327
13	31.209730737086065	21.578479921575134	30.00270179681615	17.209087544522653
14	20.722442036271985	23.46959217419944	29.820124360263506	25.987841429265067
15	22.47579660563128	29.77850601792499	28.070359251665337	19.67533812477839
16	20.909918601701307	28.55648865366603	28.562066869713732	21.971525874918935
17	18.33312233124805	27.88690870303567	28.757886506101354	25.022082459614932
18	19.263083705739888	26.835293712477405	34.57033912158134	19.33128346020137
19	18.608686204004577	25.164787777983122	36.37414016650247	19.85238585150983
20	23.6580873703853	23.67099002663477	34.79657216198557	17.87435044099436
21	22.893386709584977	24.97241208367712	33.118208218798	19.015992987939896
22	20.563875181837716	24.8261658107743	34.082657520332596	20.527301487055386
23	19.620671607510896	27.171926924399653	35.22080761897001	17.98659384911944
24	17.772487365340652	26.373950992219115	33.40837246573156	22.44518917670867
25	19.363976656863557	27.28902095517491	34.968575241160835	18.3784271468007
26	20.976905700500247	25.982263213217365	33.231809801265136	19.809021285017252
27	19.127460296440955	27.69322334061412	32.80335430257505	20.37596206036988
28	19.13289299380915	27.250215973973503	32.757030856265864	20.859860175951486
29	17.83918342678058	27.168822525903536	33.86801746806229	21.123976579253593
30	17.276220162001096	27.13287941206573	36.65964781569186	18.931252610241316
31	19.201480798082645	26.576755525222996	34.751315852659424	19.47044782403493
32	20.333567618407336	27.02097554752616	32.47656785463266	20.168888979433845
33	21.447173566434856	26.924981725279167	31.703815160233045	19.924029548052935
34	19.240285779284058	30.222143965510135	31.757754084103006	18.7798161711028
35	17.49541979956257	31.192074470639426	32.044183351595926	19.268322378202075
36	18.803778246994675	26.862311680638882	34.5994428574824	19.734467214884038
37	18.782241482427892	27.204959664647355	33.53041413161	20.48238472131475
38	19.375278607638467	26.853920103454083	34.140573954775704	19.630227334131746
39	20.61621340023312	26.054246453345986	33.814806137589855	19.514734008831045
40	18.359558224691515	26.978726624243123	33.03987066299766	21.62184448806771
41	19.281079515772042	26.36876082598343	32.936455388095894	21.413704270148635
42	16.430320078036818	28.34927005405049	33.39716752740966	21.82324234050304
43	17.045136498946686	28.05731107673636	33.08386581043475	21.81368661388219
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	321.0
2	440.0
3	1167.5
4	1895.0
5	1895.0
6	3379.5
7	4864.0
8	5499.0
9	6134.0
10	9036.5
11	11939.0
12	11939.0
13	21416.5
14	30894.0
15	51821.0
16	72748.0
17	71308.5
18	69869.0
19	69869.0
20	73969.0
21	78069.0
22	61754.0
23	45439.0
24	48027.5
25	50616.0
26	50616.0
27	53469.0
28	56322.0
29	56623.0
30	56924.0
31	59809.0
32	62694.0
33	62694.0
34	67952.5
35	73211.0
36	79024.5
37	84838.0
38	86688.0
39	88538.0
40	88538.0
41	94162.5
42	99787.0
43	115057.0
44	130327.0
45	174630.0
46	218933.0
47	218933.0
48	221601.5
49	224270.0
50	201376.0
51	178482.0
52	160529.5
53	142577.0
54	142577.0
55	121758.5
56	100940.0
57	77569.5
58	54199.0
59	45949.5
60	37700.0
61	37700.0
62	32592.5
63	27485.0
64	23073.0
65	18661.0
66	15415.5
67	12170.0
68	12170.0
69	10097.0
70	8024.0
71	6696.5
72	5369.0
73	4292.5
74	3216.0
75	3216.0
76	2501.5
77	1787.0
78	1344.0
79	901.0
80	733.0
81	565.0
82	565.0
83	415.0
84	265.0
85	207.0
86	149.0
87	119.5
88	90.0
89	90.0
90	66.0
91	42.0
92	28.0
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2061591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.20492997045352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38833312422558	23.040347421564107
2	14.040630790376058	9.324363242708609
3	5.965040750876511	5.942062812112681
4	3.157549108604538	4.193847881179265
5	1.8380994898605552	3.0516982419773035
6	1.1848618660117316	2.360595317134826
7	0.8280277608250444	1.9246222668250923
8	0.5694087944985028	1.5125743316706517
9	0.4217641438792409	1.2604183975402627
>10	2.2491655647027673	13.81756228984066
>50	0.20400872343978513	4.734158416104868
>100	0.12417147147593446	7.876888881350573
>500	0.013583335880562429	2.986235407648358
>1k	0.01299275605966841	8.134442105713791
>5k	0.0016240945074585513	3.6967115622261097
>10k+	7.382247761175233E-4	6.143471424402926
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35326	1.7135309574013466	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34724	1.6843302090472843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29841	1.4474743050391663	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14426	0.6997508235144605	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10995	0.533325960386905	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9403	0.45610404779609537	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9353	0.4536787364710071	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7814	0.3790276538847909	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7223	0.3503604740222479	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7009	0.3399801415508702	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6922	0.33576009984521665	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6223	0.301854247520483	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5974	0.2897761971215435	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5190	0.2517473155441598	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5149	0.24975856025758747	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5144	0.24951602912507861	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4955	0.2403483523162451	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4387	0.21279681566324263	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4248	0.20605445017949728	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3659	0.17748428276995776	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3552	0.1722941165342689	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3448	0.16724946897808537	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3346	0.16230183387490535	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3089	0.14983573366395178	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3061	0.14847755932190237	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2898	0.14057104440211468	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2862	0.13882482024805115	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2725	0.13217946721730936	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2661	0.1290750687211964	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2650	0.12854150022967697	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2625	0.12732884456713287	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2614	0.12679527607561344	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2567	0.1245154834300305	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2381	0.11549332530070222	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2379	0.11539631284769869	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2371	0.11500826303568457	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2350	0.1139896322791475	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA	2215	0.10744129170140926	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2209	0.10715025434239867	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	2149	0.10423988075229276	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2107	0.10220261923921865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.455186795052947E-4	0.0
9	0.0	0.0	0.0	1.455186795052947E-4	0.0
10	0.0	0.0	0.0	1.455186795052947E-4	0.0
11	0.0	0.0	0.0	1.455186795052947E-4	0.0
12	0.0	0.0	0.0	1.940249060070596E-4	0.0
13	0.0	0.0	0.0	3.395435855123543E-4	0.0
14	0.0	0.0	0.0	5.820747180211788E-4	0.0
15	0.0	0.0	0.0	9.701245300352979E-4	0.0
16	0.0	0.0	0.0	0.0015521992480564768	0.0
17	0.0	0.0	0.0	0.0027648549106005994	0.0
18	0.0	0.0	0.0	0.0031043984961129536	0.0
19	0.0	0.0	0.0	0.0037349794406358974	0.0
20	0.0	0.0	0.0	0.005093153782685315	0.0
21	0.0	0.0	0.0	0.00693639038975238	0.0
22	0.0	0.0	0.0	0.010622863603886513	0.0
23	0.0	0.0	0.0	0.018480872297172428	0.0
24	0.0	0.0	0.0	0.02992834175158894	0.0
25	0.0	0.0	0.0	0.03570058270529897	0.0
26	0.0	0.0	0.0	0.047875645557241955	0.0
27	0.0	0.0	0.0	0.13892183270105468	0.0
28	0.0	0.0	0.0	0.2372439538201321	0.0
29	0.0	0.0	0.0	0.3485172374151808	0.0
30	0.0	0.0	0.0	0.5728100287593417	0.0
31	0.0	0.0	0.0	0.892757098764983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6925	0.0	31.97762	1
GTATTGG	435	0.0	22.965515	1
ATCACGT	45	0.003826823	20.555555	23
CTAACGC	65	6.905526E-5	19.923077	3
TCTAACG	65	6.905526E-5	19.923077	2
ATTGGAC	485	0.0	19.835052	3
CAATGCG	75	9.2716255E-6	19.733334	19
CACGGCT	295	0.0	19.440676	4
ATCCACG	315	0.0	18.793652	1
TATTGGA	555	0.0	18.666666	2
CGTGACT	50	0.0070367297	18.5	12
AATGCGT	50	0.0070367297	18.5	35
TCTGTCG	60	9.2402095E-4	18.5	8
TCCACGG	325	0.0	18.215384	2
ACGGCTC	305	0.0	18.196722	5
GTATCAA	12355	0.0	17.95346	2
TTTTTAC	1650	0.0	17.715153	1
CGTCTGT	1045	0.0	17.526316	34
CTCTCGA	95	3.6091988E-6	17.526316	1
CGACGAG	75	2.0682698E-4	17.266666	24
>>END_MODULE