FastQCFastQC Report
Wed 25 May 2016
SRR2088173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174977
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT405241.2763557027342245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT403141.269741481591835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT354631.1169529732026406No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG211470.6660520690386104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136590.4302078408756977No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA120240.3787114048385232No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT109690.3454828176708052No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT109110.34365603278385953No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA104080.32781339833327927No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA90930.2863957754654601No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT84470.26604917137982415No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT79760.25121441824617946No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG79190.24941912965038804No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA66380.2090723806818128No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65650.20677315142755365No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA64390.20280461874212No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT61740.1944581015862477No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.1548357673142199No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC46810.14743413889297466No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46060.14507191705640704No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41520.13077260087238426No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG41090.12941826035275217No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40430.12733950513657263No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38320.12069378770302903No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT37330.11757565487875975No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.10979607096366369No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.10286688690973195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94700.029.010031
GTATTGG11600.023.762931
ATTGGAC11300.023.4115053
TTGGACC18050.020.8060934
TATTGGA13300.020.1691722
GGACCCT18350.020.0626726
TGGACCC19250.019.1246765
CGTCTGT8300.018.534
CGTCGTA852.7255646E-517.41176410
GTATCAA165850.016.642752
GACCCTC21000.016.5619057
ACCCTCG22400.015.8571438
CAATGCG1403.479181E-815.85714319
TATACCG2350.015.7446815
ACGTTTA2055.456968E-1215.34146326
TATACTG4250.015.2352945
GAACCGT750.004106525314.86
GTATACG2006.184564E-1114.7999991
CCCTCGC23150.014.7840189
TAGTGCG1152.2121594E-514.4782617
Produced by FastQC (version 0.11.5)